Marine Biology

, Volume 9, Issue 3, pp 205–212 | Cite as

A study of the cement apparatus of the cypris larva of the barnacle Balanus balanoides

  • G. Walker
Article

Abstract

Two types of secretory cell have been found in the cement glands of the cypris larva of Balanus balanoides (L.). Histochemical tests show the presence of proteins, phenols and the enzyme polyphenol oxidase in both the glands and secreted cement. It is proposed that cyprid cement is composed of tanned protein, all the components of which are derived from the cement glands.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literature cited

  1. Barnes, H.: Further observations on rugophilic behaviour in Balanus balanoides (L.). Vidensk. Meddr dansk. naturh. Foren. 117, 341–348 (1955).Google Scholar
  2. Barrnett, R. J. and A. M. Seligman: Demonstration of protein bound sulphydryl and disulphide groups by two new histochemical methods. J. natn. Cancer Inst. 94, 176–183 (1952).Google Scholar
  3. Brown, C. H.: Quinone tanning in the animal kingdom. Nature. Lond. 165, p. 275 (1950).Google Scholar
  4. Casselman, W. G. B.: Histochemical technique, pp 31–44. London: Methuen & Co. Ltd. 1962.Google Scholar
  5. Crisp, D. J.: The behaviour of barnacle cyprids in relation to water movements over a surface. J. exp. Biol. 32, 568–590 (1955).Google Scholar
  6. —: Territorial behaviour in barnacle settlement. J. exp. Biol. 38, 429–446 (1961).Google Scholar
  7. — and H. Barnes: The orientation and distribution of barnacles at settlement with particular reference to surface contour. J. Anim. Ecol. 23, 142–162 (1954).Google Scholar
  8. — and P. S. Meadows: The chemical basis of gregariousness in cirripedes. Proc. R. Soc. (B.) 156, 500–520 (1962).Google Scholar
  9. —: Absorbed layers: the stimulus to settlement in barnacles. Proc. R. Soc. (B.) 158, 364–387 (1963).Google Scholar
  10. Darwin, C.: A monograph of the sub-class Cirripedia: Balanidae, Verrucidae, etc., pp 110–119. London: Ray Society 1854.Google Scholar
  11. Doochin, H. D.: The morphology of Balanus improvisus Darwin and Balanus amphitrite niveus Darwin during initial attachment and metamorphosis. Bull. mar. Sci. Gulf Caribb. 1, 15–39 (1951).Google Scholar
  12. Gabbott, P. A. and V. N. Larman: Electrophoretic examination of partially purified extracts from Balanus balanoides containing a settlement inducing factor. Proc. IV European Mar. Biol. (1971). (In press).Google Scholar
  13. Gibson, P. H. and J. A. Nott: Concerning the fourth antennular segment of the cypris larva of Balanus balanoides. Proc. IV European Mar. Biol. Symp. (1971). (In press).Google Scholar
  14. Harris, J. E.: Report on anti-fouling research 1942–44. J. Iron Steel Inst. 2, 297–333 (1946).Google Scholar
  15. Hillman, R. E. and P. F. Nace: Adhesion in biological systems, pp 113–121. Ed. by R. S. Manly. New York: Academic Press 1970.Google Scholar
  16. Knight-Jones, E. W.: Laboratory experiments on gregariousness during setting in Balanus balanoides and other barnacles. J. exp. Biol. 30, 584–598 (1953).Google Scholar
  17. — and D. J. Crisp: Gregariousness in barnacles in relation to the fouling of ships and to antifouling research. Nature, Lond. 171, p. 1109 (1953).Google Scholar
  18. — and J. Moyse: Intraspecific competition in sedentary marine animals. Symp. Soc. exp. Biol. 15, 72–95 (1961).Google Scholar
  19. Kramer, H. and G. M. Windrum: The metachromatic staining reaction. J. Histochem. Cytochem. 3, 227–237 (1955).Google Scholar
  20. Laskey, A. M.: A modification of Mayer's mucihaematin technique. Stain Technol. 25, 33–34 (1950).Google Scholar
  21. Lison, L.: Histochimie animale. In: Méthodes et problèmes, pp 139–160. Paris: Gautier-Villars 1936.Google Scholar
  22. Mazia, D., P. A. Brewer and M. Aleeat: The cytochemical staining and measurement of protein with mercuric bromophenol blue. Biol. Bull. mar. biol. Lab., Woods Hole 104, 57–67 (1953).Google Scholar
  23. Nott, J. A.: Settlement of barnacle larvae: surface structure of the antennular attachment disc by scanning electron microscopy. Mar. Biol. 2, 248–251 (1969).Google Scholar
  24. — and B. A. Foster: On the structure of the antennular attachment organ of the cypris larva of Balanus balanoides (L.). Phil. Trans. R. Soc. (B.) 256, 115–134 (1969).Google Scholar
  25. Pearse, A. G. E.: Histochemistry, theoretical and applied, 2nd Ed., London: J.& A. Churchill Ltd. 1960.Google Scholar
  26. Pease, D. C.: Histochemical techniques for electron microscopy, 2nd Ed., pp 37–41. New York: Academic Press 1964.Google Scholar
  27. Pryor, M. G. M.: Comparative biochemistry, Vol 4B, pp 371–396, Ed. by M. Florkin and H. S. Mason. New York: Academic Press 1962.Google Scholar
  28. Saroyan, J. R., Lindner, E., Dooley, C. A. and H. R. Bleile: Barnacle cement — key to second generation antifouling coatings. Ind. Engng Chem. prod. Res. Dev. 9 (2), 122–133 (1970).Google Scholar
  29. Shimizu, N. and R. Kumamoto: A lead tetra-acetate schiff method for polysaccharides in tissue sections. Stain Technol. 27, 97–106 (1952).Google Scholar
  30. Steedman, H. F.: Alcianblue 8GS: a new stain for mucin. Q. Jl microsc. Sci. 91, 477–479 (1950).Google Scholar
  31. Smyth, J. D.: A technique for the histochemical demonstration of polyphenol oxidase and its application to eggshell formation in helminths and byssus formation in Mytilus. Q. Jl microsc. Sci. 95, 139–152 (1954).Google Scholar
  32. Visscher, J. P.: Reactions of the cyprid larvae of barnacles at the time of attachment. Biol. Bull. mar. biol. Lab., Woods Hole 54, 327–335 (1928).Google Scholar
  33. Walley, L. J.: Studies on the larval structure and metamorphosis of Balanus balanoides (L.). Phil. Trans. R. Soc. (B.) 256, 237–280 (1969).Google Scholar

Copyright information

© Springer-Verlag 1971

Authors and Affiliations

  • G. Walker
    • 1
  1. 1.Marine Science LaboratoriesMenai BridgeUK

Personalised recommendations