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Mammalian Genome

, Volume 1, Issue 3, pp 165–170 | Cite as

B2-like repetitive sequence from the X Chromosome of the American mink (Mustela vison)

  • Marina V. Lavrentieva
  • Mark I. Rivkin
  • Alexander G. Shilov
  • Marina L. Kobetz
  • Igor B. Rogozin
  • Oleg L. Serov
Original Contributions

Abstract

In analysis of the repeats from the mink X Chromosome (Chr), we have identified a B2-like repetitive sequence of 195 base pairs (bp) flanked by short direct repeats of 14 bp. It contains regions homologous to the split intragenic RNA polymerase III promoter and a 3′ A-rich region followed by an oligo(dA) sequence. A feature of the repeat is the presence of a perfect polypyrimidine tract 22 bp in length absent from the known Alu- and Alu-like sequences. Alignment of the mink B2-like sequence and mouse B2-consensus sequence allowed us to estimate their similarity as 55%. The repeat is present in 1–2×105 copies per mink genome and 2–4×103 copies per X Chr. In situ hybridization analysis demonstrated a similar distribution pattern of the B2-like repeat along the length of all the mink chromosomes including the X. We also observed the presence of mink B2-like hybridizable sequence in the genomes of other Carnivora species.

Keywords

Distribution Pattern Base Pair Similar Distribution Hybridization Analysis Direct Repeat 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Marina V. Lavrentieva
    • 1
  • Mark I. Rivkin
    • 1
  • Alexander G. Shilov
    • 1
  • Marina L. Kobetz
    • 1
  • Igor B. Rogozin
    • 1
  • Oleg L. Serov
    • 1
  1. 1.Siberian DepartmentInstitute of Cytology and Genetics, Academy of Sciences of the USSRNovosibirsk-90USSR

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