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Marine Biology

, Volume 12, Issue 2, pp 184–188 | Cite as

Preparative and analytical extraction of pigments from brown algae with dimethyl sulfoxide

  • G. R. Seely
  • M. J. Duncan
  • W. E. Vidaver
Article

Abstract

Dimethyl sulfoxide (DMSO) rapidly extracts much of the chlorophyll c and fucoxanthin from the intact thalli of brown algae. Subsequent extraction by acetone rapidly removes most of the chlorophyll a and β-carotene. An analytical procedure for these pigments has been developed, based upon this extraction sequence, followed by partition of the acetone extract between hexane and aqueous acetone. Fucoxanthin and chlorophyll c can be recovered from the DMSO extract and the aqueous acetone phase by extraction into ethyl acetate and subsequent evaporation. The DMSO-acetone extraction sequence is also effective with green algae and a few red algae, although the full scope of the method has not been investigated.

Keywords

Chlorophyll Ethyl Acetate Dimethyl Sulfoxide Extraction Sequence Brown Alga 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • G. R. Seely
    • 1
  • M. J. Duncan
    • 2
  • W. E. Vidaver
    • 2
  1. 1.C.F. Kettering Research LaboratoryYellow SpringsUSA
  2. 2.Department of Biological SciencesSimon Fraser UniversityBurnabyCanada

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