Marine Biology

, Volume 12, Issue 2, pp 184–188 | Cite as

Preparative and analytical extraction of pigments from brown algae with dimethyl sulfoxide

  • G. R. Seely
  • M. J. Duncan
  • W. E. Vidaver


Dimethyl sulfoxide (DMSO) rapidly extracts much of the chlorophyll c and fucoxanthin from the intact thalli of brown algae. Subsequent extraction by acetone rapidly removes most of the chlorophyll a and β-carotene. An analytical procedure for these pigments has been developed, based upon this extraction sequence, followed by partition of the acetone extract between hexane and aqueous acetone. Fucoxanthin and chlorophyll c can be recovered from the DMSO extract and the aqueous acetone phase by extraction into ethyl acetate and subsequent evaporation. The DMSO-acetone extraction sequence is also effective with green algae and a few red algae, although the full scope of the method has not been investigated.


Chlorophyll Ethyl Acetate Dimethyl Sulfoxide Extraction Sequence Brown Alga 
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Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • G. R. Seely
    • 1
  • M. J. Duncan
    • 2
  • W. E. Vidaver
    • 2
  1. 1.C.F. Kettering Research LaboratoryYellow SpringsUSA
  2. 2.Department of Biological SciencesSimon Fraser UniversityBurnabyCanada

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