Marine Biology

, Volume 119, Issue 1, pp 99–104

Isolation of Oscillatoria spongeliae, the filamentous cyanobacterial symbiont of the marine sponge Dysidea herbacea

  • R. Hinde
  • F. Pironet
  • M. A. Borowitzka


The tropical marine sponge Dysidea herbacea (Keller) (Dictyoceratidae: Dysideidae) is always found associated with the filamentous cyanobacterium (blue-green alga) Oscillatoria spongeliae (Schulze) Hauck (Cyanophyceae: Oscillatoriaceae), which occurs abundantly throughout the sponge mesohyl. Intact, metabolically active, trichomes of O. spongeliae were isolated from the sponge by chopping the sponge tissue with a razor blade and squeezing the trichomes into a seawater-based medium containing polyvinylpyrrolidone, bovine serum albumin, dithiothreitol, glycerol, KCl and Na2CO3. The isolated cyanobacteria were concentrated by centrifugation and then washed several times in fresh medium. The isolated O. spongeliae have photosynthetic rates which are similar to the intact sponge-alga association for periods of at least 6 h after isolation. Addition of sponge homogenate to the isolated cyanobacteria causes rapid cell lysis.


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  1. Berthold RJ, Borowitzka MA, Mackay MA (1982) The ultrastructure of Oscillatoria spongeliae, the blue-green algal endosymbiont of the sponge Dysidea herbacea. Phycologia 21: 327–335.Google Scholar
  2. Borowitzka MA (1988) Algal growth media and sources of cultures. In: Borowitzka MA, Borowitzka LJ (eds) Micro-algal biotechnology. Cambridge University Press, Cambridge, p 456–465Google Scholar
  3. Borowitzka MA, Hinde R, Pironet F (1989) Carbon fixation by the sponge Dysidea herbacea and its endosymbiont Oscillatoria spongeliae. In: Choat JH, Barnes DJ, Borowitzka MA, Coll JC, Davies PJ, Flood P, Hatcher BG, Hopley D, Hutchings PA, Kinsey D, Orme GR, Pichon M, Sale PF, Sammarco PW, Wallace CC, Wilkinson CR, Wolanski E, Bellwood O (eds) Proceedings of the Sixth International Coral Reef Symposium, Townsville. Sixth International Coral Reef Symposium Executive Committee, Townsville, p 151–156Google Scholar
  4. Carpenter JH (1966) New measurements of oxygen solubility in pure and natural waters. Limnol Oceanogr 11: 264–277Google Scholar
  5. Carté B, Faulkner DJ (1981) Polybrominated diphenyl ethers from Dysidea herbacea, Dysidea chlorea and Phyllospongia foliaescens. Tetrahedron 37: 2335–2339Google Scholar
  6. Charles C, Braekman JC, Daloze D, Tursch B, Karlsson R (1978) Chemical studies of marine invertebrates. XXXII. Isodysidenin, a futher hexachlorinated metabolite from the sponge Dysidea herbacea. Tetrahedron Lett 17: 1519–1520Google Scholar
  7. Cheshire AC, Wilkinson CR (1991) Modelling the photosynthetic production by sponges on Davies Reef, Great Barrier Reef. Mar Biol 109: 13–18Google Scholar
  8. Crumeyrolles-Duclaux C (1970) Sur la position systematique des zooxanthelles de Cliona viridis (Schm.), Spongiaire. Cr hebd Séanc Acad Sci, Paris 270: 1238–1239Google Scholar
  9. De Laubenfels MW (1954) The sponges of the West Central Pacific. Ore St Monogr Stud Zool 7: 1–306Google Scholar
  10. Dunlop RW, Kazlauskas R, March G, Murphy PT, Wells RJ (1982) New furano sesquiterpenes from the sponge Dysidea herbacea. Aust J Chem. 35: 95–103Google Scholar
  11. Grant BR, Borowitzka MA (1984) Changes in isolated chloroplasts of Codium fragile and Caulerpa filiformis in response to osmotic shock and detergent treatment. Protoplasma 120: 155–164Google Scholar
  12. Hinde R (1983) Host release factors in symbioses between algae and invertebrates. Endocytobiology 2: 709–726Google Scholar
  13. Hofheinz W, Oberhänsli WE (1977) Dysidenin, a novel chlorine containing natural product from the sponge Dysidea herbacea. Helv chim Acta 60: 660–669Google Scholar
  14. Jeffrey SW, Humphrey GF (1975) New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural populations. Biochem Physiol Pfl 167: 191–194Google Scholar
  15. Kazlauskas R, Lidgard RO, Wells RJ, Vetter W (1977) A novel hexachloro-metabolite from the sponge Dysidea herbacea. Tetrahedron Lett 36: 3183–3186Google Scholar
  16. Larkum AWD, Cox GC, Hiller RG, Parry DL, Dibbayawan TP (1987) Filamentous cyanophytes containign phycourobilin and in symbiosis with sponges and an ascidian of coral reefs. Mar Biol 95: 1–13Google Scholar
  17. Larkum AWD, Wyn Jones RG (1979) Carbon dioxide fixation by chloroplasts isolated by glycinebetaine. A putative cytoplasmic osmoticum. Planta 145: 393–394Google Scholar
  18. Norton RS, Croft KD, Wells RJ (1981). Polybrominated oxydiphenol derivatives from the sponge Dysidae herbacea. Structure determination by analysis of 13C spin-lattice relaxation data for quaternary carbons and 13C−H coupling constants. Tetrahedron 37: 2341–2349Google Scholar
  19. Price IR, Fricker RL, Wilkinson CR (1984) Ceratodictyon spongiosum (Rhodophyta), the macroalgal partner in an alga-sponge symbiosis, grown in unialgal culture. J Phycol 20: 156–158Google Scholar
  20. Rützler K (1990) Associations between Caribbean sponges and photosynthetic organisms. In: Rützler K (ed) New perspectives in sponge biology. Smithsonian Institution Press, Washington, DC, p 455–466Google Scholar
  21. Scott FJ, Wetherbee R (1982) The structure of sponge — red alga associations observed in selected seaweeds from Southern and Western Australia. Micron 13: 331–332Google Scholar
  22. Sharma GM, Vig B (1972) Studies on the antimicrobial substances of sponges. VI. Structures of two antibacterial substances isolated from the marine sponge Dysidea herbacea. Tetrahedron Lett 17: 1715–1718Google Scholar
  23. Smith RV, Foy RH (1974) Improved hydrogen ion buffering of media for the culture of freshwater algae. Br Phycol J 9: 239–245Google Scholar
  24. Spurr AR (1969) Low viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26: 31–43Google Scholar
  25. Wilkinson CR (1983) Net primary productivity in coral reef sponges. Science, NY 219: 410–412Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • R. Hinde
    • 1
  • F. Pironet
    • 1
  • M. A. Borowitzka
    • 2
  1. 1.School of Biological Sciences, A12University of SydneyAustralia
  2. 2.School of Biological and Environmental SciencesMurdoch UniversityMurdochAustralia

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