Marine Biology

, Volume 119, Issue 2, pp 205–210 | Cite as

Integumental ultrastructure and color patterns in the iridescent copepods of the family Sapphirinidae (Copepoda: Poecilostomatoida)

  • J. Chae
  • S. Nishida


The ultrastructure of the integument of the sapphirinid copepods was studied by scanning and transmission electron microscopy. Samples were collected between 1991 and 1993 by plankton-net tows from the subtropical and tropical waters of the North Pacific. In all the seven species examined of Sapphirina and Copilia, a structure with multilayered platelets was found in the epidermal cells of the dorsal integument of the male. Each platelet is a regular hexagonal prism. The platelets form a plate with honeycomb arrangement within each epidermal cell. Just ventral to the dorsal cuticle, 10 to 14 plates are located parallel to each other and to the cuticle. The mean diameter and thickness of the platelets measured between 1.0 and 1.8 μm and 61 and 83 nm, respectively, for the four species. The specific coloration of seven species was examined with reflected and transmitted light. The iridescent color may be explained by the theory of multiple thin-layer interference in some species which are considered to have an ideal laminar structure, but for the other species, mechanisms from non-ideal systems, including pigment-thin layer interaction, may also be involved.


Color Microscopy Electron Microscopy Transmission Electron Microscopy Hexagonal 
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  1. Boxshall GA (1977) The depth distribution and community organization of the planktonic cyclopoids (Crustacea: Copepoda) in the Cape Verde Islands regions. J mar biol Ass UK 57: 543–568Google Scholar
  2. Dana JD (1852) Crustacea. US Exploring Expedition, Vol. 13 C. Sherman, PhiladelphiaGoogle Scholar
  3. Davis CC (1955) The marine and fresh-water plankton. Michigan State University Press. ChicagoGoogle Scholar
  4. Denton EJ (1970) On the organization of reflecting surfaces in some marine animals. Phil Trans R Soc Lond (Ser B) 258: 285–313Google Scholar
  5. Denton EJ, Land MK (1971) Mechanism of reflexion in silvery layers of fish and cephalopods. Proc R Soc Lond (Ser A) 178: 43–61Google Scholar
  6. Elofsson R (1969) The ultrastructure of the nauplius eye of Sapphirina (Crustacea: Copepoda). Z Zellforsch 100: 376–401Google Scholar
  7. Furuhashi K (1966) Droplets from the plankton net. XXIII. Record of Sapphirina salpae Giesbrecht from the North Pacific, with notes on its copepodite stages. Publs Seto mar biol Lab 14: 123–127Google Scholar
  8. Gregory RL, Ross HE, Moray N (1964) The curious eye of Copilia. Nature, Lond 201: 1166–1168Google Scholar
  9. Hardy A (1936) Observation on the uneven distribution of oceanic plankton Disc Rep 11: 511–536Google Scholar
  10. Heron AC (1973) A specialized predator-prey relationship between the copepod Sapphirina angusta and the pelagic tunicate Thalia democratica. J mar biol Ass UK 53: 429–435Google Scholar
  11. Ho JS (1991) Phylogeny of Poecilostomatoida: a major order of symbiotic copepods. Bull Plankton Soc Japan (Spec Vol): 25–48Google Scholar
  12. Huxley AF (1968) A theoretical treatment of the reflexion of light by multilayer structures. J exp Biol 48: 227–245Google Scholar
  13. Kasukawa H, Oshima N, Fujii R (1987) Mechanism of the light reflection in blue damselfish motile iridophore. Zool Sci 4: 243–257Google Scholar
  14. Land MF (1966) A multilayer interference reflector in the eye of the scallop, Pecten, maximus. J exp Biol 45: 433–447Google Scholar
  15. Land MF (1984) Crustacea. In: Ali MA (ed) Photoreception and vision in invertebrates. Plenum Press, New York, pp 408–417Google Scholar
  16. Lehnhofer K (1926) Copepoda: Copilia Dana 1849, Systematik und Verbreitung der Gattung. Deutsche Tiefsee-Expedition 1898–1899 23 (3): 1–64Google Scholar
  17. Lehnofer K (1929) Copepoda: Sapphirina J. V. Thompson 1829, Systematik und Verbreitung der Gattung. Deutsche Tiefsee-Expedition 1898–1899 23 (5): 1–80Google Scholar
  18. Menter DG, Obika M, Tchen TT, Taylor D (1979) Leucophores and iridophores of Fundulus heteroclitus: biophysical and ultrastructural properties. J Morphol 160: 103–120Google Scholar
  19. Nagaishi H, Oshima N (1992) Ultrastructure of the motile iridophores of the neon tetra. Zool Sci 9: 65–75Google Scholar
  20. Nicol JAC (1960) The biology of marine animals. Sir Isaac Pitman and Sons, LondonGoogle Scholar
  21. Rajaram LK, Krishnaswamy S (1981) Distribution of Sapphirina (Copepoda, Cyclopoida) in the south-east Indian Ocean along 110oE. Bull Dep mar Sci Univ Cochin 12: 1–22Google Scholar
  22. Rohrlich ST, Porter KR (1972) Fine structural observations relating the production of color by the iridophores of a lizard, Anolis caroliniensis. J Cell Biol 53: 38–52Google Scholar
  23. Rose M, Vaissière R (1951) Le système excréto-glandulaire des Sapphirines. Archs Zool exp gén 87: 134–138Google Scholar
  24. Schmidt WJ (1949) Altes und Neues über Strukturfarben im Tierreich. Giessener naturw Vortr 6: 30–37Google Scholar
  25. Sewell RBS (1947) The free-swimming planktonic copepoda, systematic account. Scient Rep John Muray Exped 8 (1): 1–303Google Scholar
  26. Toda T, Suh H, Nemoto T (1989) Dry fracturing: a simple technique for scanning electron microscopy of small crustaceans and its application to internal observations of copepods. J Crustacean Biol 9: 409–413Google Scholar
  27. Wolken JJ, Florida RG (1969) The eye structure and optical system of the crustacean copepod, Copilia. J Cell Biol 40: 279–285Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • J. Chae
    • 1
  • S. Nishida
    • 1
  1. 1.Ocean Research InstituteUniversity of TokyoNakano, TokyoJapan

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