Marine Biology

, Volume 121, Issue 1, pp 127–135 | Cite as

Gametic incompatibility and genetic divergence of Pacific and Kumamoto oysters, Crassostrea gigas and C. sikamea

  • M. A. Banks
  • D. J. McGoldrick
  • W. Borgeson
  • D. Hedgecock
Article

Abstract

The biological and taxonomic separation of the Pacific oyster Crassostrea gigas (Thunberg, 1793) from the Kumamoto oyster C. sikamea (Amemiya, 1928) is affirmed by three concordant lines of evidence: (1) fixed differences in 2% of a mtDNA sequence coding for large subunit rRNA; (2) a genetic distance of 0.440 based on 19 allozyme loci, including 5 diagnostic loci (Aat-1, Idh-1, Idh-2, Mpi-1, Mdh-2); (3) one-way gametic incompatibility resulting in partial reproductive isolation in interspecific crosses. C. gigas sperm x C. sikamea egg fertilizations form viable hybrid offspring, but C. sikamea sperm do not fertilize C. gigas eggs. Divergence between these two species is mediated by differing peaks in the periods for gamete release and by one-way sperm/egg incompatibility. Two attempts to recover C. sikamea from its place of origin in southern Japan have yielded only individuals with the mitochondrial haplotype that characterizes C. gigas. We thus identify a crucial need for careful screening, management, and conservation of the cultivated populations of C. sikamea on the US west coast.

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • M. A. Banks
    • 1
  • D. J. McGoldrick
    • 1
  • W. Borgeson
    • 1
  • D. Hedgecock
    • 1
  1. 1.Bodega Marine LaboratoryThe University of California at DavisBodega BayUSA

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