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Marine Biology

, Volume 121, Issue 4, pp 655–664 | Cite as

Molecular systematics of six Calanus and three Metridia species (Calanoida: Copepoda)

  • A. Bucklin
  • B. W. Frost
  • T. D. Kocher
Article

Abstract

The discrimination of species of the copepod genus, Calanus (Copepoda; Calanoida), is problematical-especially in regions of sympatry. Although the species of Calanus exhibit exceptional morphological similarity, they are quite distinct in genetic character. The DNA base sequences of the mitochondrial large subunit (16S) ribosomal RNA (rRNA) gene unambiguously discriminated C. finmarchicus (Gunnerus 1765), C. glacialis (Jaschnov 1955), C. marshallae (Frost 1974), C. helgolandicus (Claus 1863), C. pacificus (Brodsky 1948), C. sinicus (Brodsky 1965), and C. hyperboreus (Kroyer 1838). Sequence differences among Calanus species for this gene portion range from 7.3% (between C. glacialis and C. marshallae) to 23.9% (between C. glacialis and C. sinicus). Differences among conspecific individuals were approximately 1 to 2%. [These sequence data were determined between April and November 1993; the sequenced domain is similar to that published previously in Bucklin et al. (1992) but are derived from analysis of additional individuals.] Statistical analysis of the sequence data using a variety of tree-building algorithms separated the taxa into one group of species corresponding to the C. finmarchicus group (C. finmarchicus, C. marshallae, and C. glacialis) and another ungrouped set of species corresponding to the C. helgolandicus group (C. helgolandicus, C. pacificus, and C. sinicus). The C. helgolandicus group may be older than the C. finmarchicus group, making the tree topology less reliable in this area. Calanus hyperboreus was an outlier; Nannocalanus minor (Claus 1863) was the outgroup. Similar analysis of Metridia species confirmed that M. lucens (Boeck 1864) and M. pacifica (Brodsky 1948) are distinct species; M. longa (Lubbock 1854) was still more divergent. These sequence data will allow the design of simple, molecular tools for taxonomic identifications. Diagnostic characters, assayed by rapid molecular protocols, will enable biological oceanographers to answer important questions about the distribution and abundance of all life stages (as well as patterns of reproduction) of morphologically similar species, such as those of Calanus.

Keywords

Sequence Data Tree Topology Large Subunit Distinct Species Diagnostic Character 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Alldredge AL, Robison BH, Fleminger A, Torres JJ, King JM, Hamner WM (1984) Direct sampling and in situ observation of a persistent copepod aggregation in the mesopelagic zone of the Santa Barbara Basin. Mar Biol 80:75–81Google Scholar
  2. Avise JC, Arnold J, Ball RM, Bermingham E, Lamb T, Neigel JE, Reeb CA, Saunders NC (1987) Intraspecific phylogeography: the mitochondrial DNA bridge between population genetics and systematics. A Rev Ecol Syst 18:489–522Google Scholar
  3. Avise JC, Gibling-Davidson C, Laerm J, Patton JC, Lansman RA (1979) Mitochondiral DNA clones and matriarchal phylogeny within and among geographic populations of the pocket gopher, Geomys pinetis. Proc natn Acad Sci USA 76:6694–6698Google Scholar
  4. Ayala FJ, Tracy ML, Barr LG, MacDonald JF, Perez-Salas S (1974) Genetic variation in natural populations of five Drosophila species and the hypothesis of the selective neutrality of protein polymorphisms. Genetics 77:343–348Google Scholar
  5. Birky CW, Fuerst P, Maruyama T (1989) Organelle gene diversity under migration, mutation, and drift: equilibrium expectations, approach to equilibrium, effects of heteroplasmic cells, and comparison to nuclear genes. Genetics 121:613–627Google Scholar
  6. Bradford JM (1988) Review of the taxonomy of the Calanidae (Copepoda) and the limits to the genus Calanus. Hydrobiologia 167/168:73–81Google Scholar
  7. Bradford JM, Jillette JB (1974) A revision of generic definitions in the Calanidae (Copepoda, Calanoida). Crustaceana 27:5–16Google Scholar
  8. Brasher DJ, Ovenden JR, White RWG (1992) Mitochondiral DNA variation and phylogenetic relationships of Jasus spp. (Decapoda: Palinuridae). J Zool Lond 227:1–16Google Scholar
  9. Brodsky KA (1948) Free living Copepoda of the Sea of Japan. Izv tikhookean n auch no-issled Inst ryb Khoz Okeanogr 26:3–130Google Scholar
  10. Brodsky KA (1965) Variability and systematics of the species of the genus Calanus (Copepoda). I Calanus pacificus Brodsky, 1948 and C. sinicus Brodsky, sp n. Issled Fauny Morei 3:22–71 (in Russian)Google Scholar
  11. Brodsky KA (1967a) Formation of swimming limbs in the genus Calanus (Copepoda) and latitudinal zonality. Dokl Akad Nauk SSSR 176:1441–1444 (in Russian)Google Scholar
  12. Brodsky KA (1967b) Types of female genitalia and heterogeneity in the genus Calanus (Copepoda). Dokl Akad Nauk SSSR 176: 222–225 (in Russian)Google Scholar
  13. Brodsky KA (1972) Phylogeny of the fam. Calanidae (Copepoda) on the basis of comparative-morphological analysis of its characters. Issled Fauny Morei 12:1–110 (in Russian)Google Scholar
  14. Bucklin A, Frost BW, Kocher TD (1992) DNA sequence variation of the mitochondrial 16S rRNA in Calanus (Copepoda; Calanoida): intraspecific and interspecific patterns. Molec mar Biol Biotechnol 1:397–407Google Scholar
  15. Bucklin A, LaJeunesse TC (1994) Molecular genetic variation of Calanus pacificus (Copepoda; Calanoida): preliminary evaluation of genetic structure and sub-specific differentiation based on mtDNA sequences. Calif coop ocean Fish Invest Rep 35:45–51Google Scholar
  16. Clary DO, Wolstenholme DR (1985) The mitochondrial DNA molecule of Drosophila yakuba: nucleotide sequence, gene organization, and genetic code. J molec Evolut 22:252–271Google Scholar
  17. Cox JL, Willason S, Harding L (1983) Consequences of distributional heterogeneity of Calanus pacificus grazing. Bull mar Sci 33:213–226Google Scholar
  18. Cunningham CW, Blackstone NW, Buss LW (1992) Evolution of king crabs from hermit crab ancestors. Nature, Lond 355: 539–542Google Scholar
  19. DeDecker AHB, Kaczmaruk BZ, Marska G (1991) A new species of Calanus (Copepoda, Calanoida) from South African waters. Ann S Afr Mus 101:27–44Google Scholar
  20. Devereux J, Haeberli P, Smithies O (1984) A comprehensie set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387–395Google Scholar
  21. Ehrlich HH, Bugawan TL (1990) HLA DNA typing. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 261–271Google Scholar
  22. Feng D-F, Doolittle RF (1987) Progressive sequence alignment as a prerequisite to correct phylogenetic trees. J molec Evolut 25: 351–360Google Scholar
  23. Fleminger A (1964) Distributional atlas of calanoid copepods in the California Current region, Part I. Calif coop ocean Fish Invest Atlas 2:1–213Google Scholar
  24. Fleminger A (1985) Dimorphism and possible sex change in copepods of the family Calanidae. Mar Biol 88:273–294Google Scholar
  25. Fleminger A, Hulsemann K (1977) Geographical range and taxomonic divergence in North Atlantic Calanus (C. helgolandicus, C. finmarchicus, and C. glacialis). Mar Biol 40:233–248Google Scholar
  26. Fleminger A, Hulsemann K (1987) Geographical variation in Calanus helgolandicus s.1. (Copepoda, Calanoida) and evidence of recent speciation of the Black Sea population. Biol Oceanogr (NY) 5:43–81Google Scholar
  27. Frost BW (1974) Taxonomic status of Calanus finmarchicus and C. glacialis (Copepoda), with special reference to adult males. J Fish Res Bd Can 28:23–30Google Scholar
  28. Frost BW (1974) Calanus marshallae, a new species of calanoid copepod closely allied to the sibling species C. finmarchicus and C. glacialis. Mar Biol 26:77–99Google Scholar
  29. Grainger EH (1961) The copepods Calanus glacialis and Calanus finmarchicus (Gunnerus) in Canadian Arctic-Subarctic waters. J Fish Res Bd Can 18:663–678Google Scholar
  30. Hulsemann K (1991) Calanus euxinus, new name, a replacement name for Calanus ponticus Karavaev, 1894 (Copepoda: Calanoida). Proc biol Soc Wash 104:620–621Google Scholar
  31. Jaschnov WA (1955) Morphology, distribution, and systematics of Calanus finmarchicus s. l. Zool Zh 34:1210–1223 (in Russian)Google Scholar
  32. Jaschnov WA (1957) Comparative morphology of the species Calanus finmarchicuss.l. Zool Rh 36:191–198 (in Russian)Google Scholar
  33. Kumar S, Tamura K, Nei M (1993) MEGA: molecular evolutionary genetics analysis, Version 1.0. Pennsylvania State University, University Park, PennsylvaniaGoogle Scholar
  34. Machado EG, Dennebouy N, Suarez MO, Mounolou J-C, Monnerot M (1992) Mitochondrial 16S rRNA gene of two species of shrimps: sequence variability and secondary structure. Crustaceana 65:279–286Google Scholar
  35. Palumbi SR, Benzie J (1991) Large mitochondrial DNA differences between morphologically similar Penaeid shrimp. Molec mar Biol Biotechnol 1:27–34Google Scholar
  36. Palumbi S, Martin A, Romano S,McMillan WO, Stice L, Grabowski G (1991) The simple fool's guide to PCR (Ver. 2) (unpublished manuscript)Google Scholar
  37. Saitou N, Nei M (1987). The neighbor-joining method: a new method for reconstructing phylogenetic tree. Molec Biol Evolut 4:406–425Google Scholar
  38. Sherman K, Smith WG, Green JR, Cohen E, Berman MS, Marti KA, Goulet JR (1987) Zooplankton production and fisheries of the northeast shelf. In: Backus RH, Bourne DW (eds) Georges Bank. MIT Press, Cambridge, Massachusetts, pp 268–282Google Scholar
  39. Skjoldal HR, Rey F (1989) Pelagic production and variability of the Barents Sea ecosystem. In: Sherman K, Alexander LM (eds) Biomass yields and geography of large marine ecosystems. AAAS Publ, Washington DC, pp 241–286Google Scholar
  40. Smith LM, Sanders JZ, Kaiser RJ (1986) Fluorescence detection in automated DNA sequence analysis. Nature, Lond 321:674–679Google Scholar
  41. Smithies O, Engles WR, Devereaux JR, Slightom JL, Shen S (1981) Base substitutions, length differences and DNA strand asymmetries in the human G-gamma and A-gamma fetal globin gene region. Cell 26:345–353Google Scholar
  42. Stoneking M, Hedgecock D, Higuchi RG, Vigilant L, Erlich HA (1991) Population variation of human mitochondrial DNA control region sequences detected by enzymatic amplification and sequence-specific oligonucleotide probes. Am J Genet 48: 370–382Google Scholar
  43. Swofford DL (1991) PAUP: phylogenetic analysis using parsimony, Version 3.0q. Illinois Natural History Survey, Champaign, IllinoisGoogle Scholar
  44. Tamura K, Nei M (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evolut 10:512–526Google Scholar
  45. Wilson AC, Cann RL, Carr SM, George M, Gyllensten UB, Helm-Bychowski KM Higuchi RG, Palumbi SR, Proger EM, Sage RD, Stoneking M (1985) Mitochondrial DNA and two perspectives on evolutionary genetics. Biol J Linn Soc 26:375–400Google Scholar
  46. Xiong B, Kocher TD (1991) Comparison of mitochondrial DNA sequences of seven morphospecies of black flies (Diptera: Simuliidae). Genome 34:306–311Google Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • A. Bucklin
    • 1
    • 2
  • B. W. Frost
    • 3
  • T. D. Kocher
    • 2
  1. 1.Ocean Process Analysis LaboratoryUniversity of New HampshireDurhamUSA
  2. 2.Department of ZoologyUniversity of New HampshireDurhamUSA
  3. 3.School of OceanographyUniversity of WashingtonSeattleUSA

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