Marine Biology

, Volume 118, Issue 4, pp 637–644 | Cite as

Ontogenetic and seasonal changes in lipid and fatty acid/alcohol compositions of the dominant Antarctic copepods Calanus propinquus, Calanoides acutus and Rhincalanus gigas

  • G. Kattner
  • M. Graeve
  • W. Hagen


Lipid compositions of the dominant Antarctic copepods Calanoides acutus, Rhincalanus gigas and Calanus propinquus from the Weddell Sea have been investigated in great detail. Copepods were collected during summer in 1985 and late spring/early winter in 1986. The analyses revealed specific adaptations in the lipid biochemistry of these species which result in very different lipid components. The various copepodite stages of C. acutus synthesize wax esters with long-chain monounsaturated moieties and especially the alcohols consisted mainly of 20:1(n-9) and 22:1(n-11). R. gigas also generates wax esters, but with moieties of shorter chain length. The fatty alcohols consisted mainly of 14:0 and 16:0 components, while the major fatty acids were 20:5, 18:4 and 22:6, of which 18:4 probably originated from dietary input. In contrast, C. propinquus accumulates triacylglycerols, a very unusual depot lipid in polar calanoid copepods. Major fatty acids in C. propinquus were the long-chain monounsaturates 22:1(n-9) and 22:1(n-11), which may comprise up to 50% of total fatty acids. In C. acutus and C. propinquus there was a clear increase of long-chain fatty acids with increasing developmental stage. In contrast, the fatty acid and alcohol composition of the R. gigas copepodite stages were characterized by the dominance of the polyunsaturated fatty acids as well as high amounts of the monounsaturates 18:1(n-9) and 16:1(n-7). There was a considerable decrease of the dietary fatty acid 18:4(n-3) towards the older stages during summer; in late winter/early spring 18:4 was only detected in very low amounts. This tendency was also found in the other two species, but was less pronounced. In all three species dry weight and lipid content increased exponentially from younger to older stages. The highest portion of wax esters, or of triacylglycerols in C. propinquus, was found in the adults. Dry weight and lipid content were generally higher during summer. In late winter/early spring the variability was more pronounced and lipid-rich specimens showed a selective retention of long-chain monounsaturated fatty acids, whereas in lipid-poor specimens these fatty acids were very much depleted.


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  1. Bathmann, U. V., Makarov, R. R., Spiridonov, V. A., Rohardt, G. (1993). Winter distribution and overwintering strategies of the Antarctic copepod species Calanoides acutus, Rhincalanus gigas and Calanus propinquus (Crustacea, Calanoida) in the Weddell Sea. Polar Biol. 13: 333–346Google Scholar
  2. Bodungen, B. v., Nöthig, E.-M., Sui, Q. (1988). New production and sedimentation during summer 1985 in the southeastern Weddell Sea. Comp. Biochem. Physiol. 90B: 475–487Google Scholar
  3. Boysen-Ennen, E., Hagen, W., Hubold, G., Piatkowski, U. (1991). Zooplankton biomass in the ice-covered Weddell Sea, Antarctica. Mar. Biol. 111: 227–235Google Scholar
  4. Clarke, A. (1983). Life in cold water: the physiological ecology of polar marine ectotherms. Oceanogr. mar. Biol. A. Rev. 21: 341–453Google Scholar
  5. Clarke, A. (1988). Seasonality in the Antarctic marine environment. Comp. Biochem. Physiol. 90B: 461–473Google Scholar
  6. Conover, R. J., Huntley, M. E. (1991). Copepods in ice-covered seas-distribution, adaptations to seasonally limited food, metabolism, growth patterns and life cycle strategies in polar seas. J. mar. Syst. 2: 1–40Google Scholar
  7. Falk-Petersen, S., Sargent, J. R., Tande, K. (1987). Lipid composition of zooplankton in relation to the sub-Arctic food web. Polar Biol. 8: 115–120Google Scholar
  8. Folch, J., Lees, M., Sloane-Stanley, G. H. (1957). A simple method for the isolation and purification of total lipides from animal tissues. J. biol. Chem. 226: 497–509Google Scholar
  9. Fraser, A. J., Tocher, D. R., Sargent, J. R. (1985). Thin-layer chromatography-flame ionization detection and the quantitation of marine neutral lipids and phospholipids. J. exp. mar. Biol. Ecol. 88: 91–100Google Scholar
  10. Gatten, R. R., Sargent, J. R., Forsberg, T. E. V., O'Hara, S. C. N., Corner, E. D. S. (1980). On the nutrition of zooplankton. XIV. Utilization of plankton by Calanus helgolandicus during maturation and reproduction. J. mar. biol. Ass. U.K. 60: 391–399Google Scholar
  11. Graeve, M. (1993). Unisatz und Verteilung von Lipiden in arktischen marinen Organismen unter besonderer Berücksichtigung unterer trophischer Stufen. Ber. Polarforsch. (Bremerhaven) 124: 1–141Google Scholar
  12. Graeve, M., Hagen, W., Kattner, G. (1994). Herbivorous or omnivorous: on the significance of lipid compositions as trophic markers in Antarctic copepods. Deep-Sea Res. (in press)Google Scholar
  13. Hagen, W. (1988). Zur Bedeutung der Lipide im antarktischen Zooplankton. Ber. Polarforsch. (Bremerhaven) 49: 1–129 (in German) [English version (1989): On the significance of lipids in Antarctic zooplankton. Can. Trans. Fish. aquat. Sciences 5458: 1–149]Google Scholar
  14. Hagen, W., Kattner, G., Graeve, M. (1993). Calanoides acutus and Calanus propinquus, Antarctic copepods with different lipid storage modes via wax esters or triacylglycerols. Mar. Ecol. Prog. Ser. 97: 135–142Google Scholar
  15. Harrington, G. W., Beach, B. H., Dunham, J. E., Holz, G. G. Jr. (1970). The polyunsaturated fatty acids of marine dinoflagellates. J. Protozool. 17: 213–219Google Scholar
  16. Hempel, G. (ed.) (1985). Die Expedition ANTARKTIS III mit FS ‘Polarstern’ 1984/85. Ber. Polarforsch. (Bremerhaven) 25: 1–209Google Scholar
  17. Hirche, H.-J., Kattner, G. (1993). Egg production and lipid content of Calanus glacialis in spring: indication of a food-dependent and food-indepedent reproductive mode. Mar. Biol. 117: 615–622Google Scholar
  18. Hopkins, T. L., Lancraft, T. M., Torres, J. J., Donnelly, J. (1993). Community structure and trophic ecology of zooplankton in the Scotia Sea marginal ice zone in winter (1988). Deep-Sea Res. 40: 81–105Google Scholar
  19. Hopkins, T. L., Torres, J. J. (1989). Midwater food web in the vicinity of a marginal ice zone in the western Weddell Sea. Deep-Sea Res. 36: 543–560Google Scholar
  20. Huntley, M., Escritor, F. (1991). Dynamics of Calanoides acutus (Copepoda: Calanoida) in Antarctic coastal waters. Deep-Sea Res. 38: 1145–1167Google Scholar
  21. Kattner, G., Fricke, H. S. G. (1986). Simple gas-liquid chromatographic method for the simultaneous determination of fatty acids and alcohols in wax esters of marine organisms. J. Chromat. 361: 263–268Google Scholar
  22. Kattner, G., Hirche, H.-J., Krause, M. (1989). Spatial variability in lipid composition of calanoid copepods from Fram Strait, the Arctic. Mar. Biol. 102: 473–480Google Scholar
  23. Kattner, G., Krause, M. (1987). Changes in lipids during the development of Calanus finmarchicus s.1. from copepodid I to adult. Mar. Biol. 96: 511–518Google Scholar
  24. Kattner, G., Krause, M. (1989). Seasonal variations of lipids (wax esters, fatty acids and alcohols) in calanoid copepods from the North Sea. Mar. Chem. 26: 261–275Google Scholar
  25. Lee, R. F. (1974). Lipid composition of the copepod Calanus hyperboreus from the Arctic ocean. Changes with depth and season. Mar. Biol. 26: 313–318Google Scholar
  26. Lee, R. F. (1975). Lipids of Arctic zooplankton. Comp. Biochem. Physiol. 51B: 263–266Google Scholar
  27. Lee, R. F., Hirota, J. (1973). Wax esters in tropical zooplankton and nekton and the geographical distribution of wax esters in marine copepods. Limnol. Oceanogr. 18: 227–239Google Scholar
  28. Marin, V., Schnack-Schiel, S. B. (1993). On the occurrence of Rhincalanus gigas, Calanoides acutus and Calanus propinquus (Copepoda: Calanoida) in late May in the area of the Antarctic Peninsula, Antarctica. Polar Biol. 13: 35–40Google Scholar
  29. Nöthig, E.-M., Bathmann, U., Jennings, J. C. Jr., Fahrbach, E., Gradinger, R., Gordon, L. I., Makarov, R. (1991). Regional relationships between biological and hydrographical properties in the Weddell Gyre in late austral winter 1989. Mar. Chem. 35: 325–336Google Scholar
  30. Sargent, J. R., Eilertsen, H. C., Falk-Petersen, S., Taasen, J. P. (1985). Carbon assimilation and lipid production in phytoplankton in northern Norwegian fjords. Mar. Biol. 85: 109–116Google Scholar
  31. Sargent, J. R., Gatten, R. R., Henderson, R. J. (1981). Lipid biochemistry of zooplankton from high latitudes. Océanis 7: 623–632Google Scholar
  32. Sargent, J. R., Henderson, R. J. (1986). Lipids. In: Corner, E. D. S., O'Hara, S. (eds.) Biological chemistry of marine copepods. Univ. Press, Oxford, p. 59–108Google Scholar
  33. Schnack-Schiel, S. (B.) (ed.) (1987). The winter expedition of RV ‘Polarstern’ to the Antarctic (ANT V/1-3) Ber. Polarforsch. (Bremerhaven) 39: 1–259Google Scholar
  34. Schnack-Schiel, S. B., Hagen, W., Mizdalski, E. (1991). Seasonal comparison of Calanoides acutus and Calanus propinquus (Copepoda: Calanoida) in the southeastern Weddell Sea, Antarctica. Mar. Ecol. Prog. Ser. 70: 17–27Google Scholar
  35. Tande, K. S., Henderson, R. J. (1988). Lipid composition of copepodite stages and adult females of Calanus glacialis in Arctic waters of the Barents Sea. Polar Biol. 8: 333–339Google Scholar

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • G. Kattner
    • 1
  • M. Graeve
    • 1
  • W. Hagen
    • 2
  1. 1.Sektion ChemieAlfred-Wegener-Institut für Polar- und MeeresforschungBremerhavenGermany
  2. 2.Institut für PolarökologieUniversität KielKielGermany

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