Advertisement

Immunogenetics

, Volume 27, Issue 3, pp 167–173 | Cite as

Serological recognition of HLA-DR allodeterminant corresponding to DNA sequence involved in gene conversion

  • Christine C. Berte
  • Nobuyuki Tanigaki
  • Roberto Tosi
  • Jack Gorski
  • Bernard Mach
Article

Abstract

HLA class 11 molecules were isolated from mouse L cells transfected with a DRα gene and an allele, 52a, of locus DR β III from an HLA-homozygous cell line, AVL, of the DR3 haplotype. The isolated molecules were found to possess a new allospecificity, named TR81. This specificity behaved allelic to the previously described DR β III locus. The TR81 specificity was also present on the DR β I gene product of the DR3 haplotype. The nucleotide sequence of the gene encoding TR81 differs from TR81-negative DR β genes of the DRw52 family in only two codons, both located in the regions known to be involved in a gene conversion event. Consequently, the following conclusions can be formulated. (a) TR81 is a bi-locus specificity and allelic to TR22 only in its DR β III locus localization. (b) The TR81 specificity is the phenotypic counterpart of the gene conversion event which led to the generation of the DR β I gene of the DR3 haplotype. (c) One or both individual amino acid substitutions in the first domain of the DR β chain are responsible for the TR81 allospecificity. (d) Since TR81 is expressed on the DR β I chain of the DR3 haplotype, it is possible that TR81 and DR3 represent the same serological specificity.

Keywords

Nucleotide Codon Nucleotide Sequence Gene Product Amino Acid Substitution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Angelini, G., de Preval, C., Gorski, J., and Mach, B.: High-resolution analysis of the human HLA-DR polymorphism by hybridization with sequence-specific oligonucleotide probes. Proc. Natl. Acad. Sci. U.S.A. 83: 4489–4493, 1986Google Scholar
  2. Berdoz, J., Gorski, J., Termijtelen, A. M., Dayer, J. M., Irle, C., Schendel, D., and Mach, B.: Constitutive and induced expression of the individual HLA-DR beta and alpha chain loci in different cell types. J. Immunol., in press, 1987Google Scholar
  3. Bontrop, R., Tilanus, M., Mikulski, M., van Eggermond, M., Termijtelen, A., and Giphafrt, M.: Polymorphisms within the HLA-DR3 haplotypes. I. HLA-DR polymorphisms detected at the protein and DNA levels are reflected by T-cell recognition. Immunogenetics 23: 401–405, 1986Google Scholar
  4. Ferrara, G. B., Tosi, R., Longo, A., Castellani, A., Viviani, C., and Carminati, G.: A safe blood transfusion procedure for immunization against major histocompatibility complex determinants in man. Transplantation 26: 150–152, 1978Google Scholar
  5. Gorski, J. and Mach, B.: Polymorphism of human la antigens: gene conversion between two DR beta loci results in a new HLA-D/DR specificity. Nature 322: 67–70, 1986Google Scholar
  6. Gorski, J., Tosi, R., Strubin, M., Rabourdin-Combe, C., and Mach, B.: Serological and immunochemical analysis of the products of a single HLA DR-alpha and DR-beta chain gene expressed in a mouse cell line after DNA-mediated cotransformation reveals that the beta chain carries a known supertypic specificity. J. Exp. Med. 162: 105–116, 1985Google Scholar
  7. Gorski, J., Tilanus, M., Giphart, M., and Mach, B.: Oligonucleotide genotyping shows that alleles at the HLA-DRβIII locus of the DRw52 supertypic group segregate independently of known DR or Dw specificities. Immunogenetics 25: 79–83, 1987Google Scholar
  8. Rabourdin-Combe, C. and Mach, B.: Expression of HLA-DR antigens at the surface of mouse L cells co-transfected with cloned human genes. Nature 303: 670–674, 1983Google Scholar
  9. Rollini, P., Mach, B., and Gorski, J.: Linkage map of three HLA-DR beta chain genes: evidence for a recent duplication event. Proc. Natl. Acad. Sci. U.S.A. 82: 7197–7201, 1985Google Scholar
  10. Rollini, P., Mach, B., and Gorski, J.: Characterization of an HLA-DR β pseudogene in the DRw52 supertypic group. Immunogenetics 25: 336–342, 1987Google Scholar
  11. Tanigaki, N. and Tosi, R.: Assessment of the specificity of human Ia alloantisera and alloantigens by the use of radioiodinated human Ia antigens. Tissue Antigens 20: 1–21, 1982Google Scholar
  12. Tanigaki, N., Tosi, R., Duquesnoy, R., and Ferrara, G. B.: Three Ia species with different structures and alloantigenic determinants in an HLA-homozygous cell line. J. Exp. Med. 157: 231–247, 1983aGoogle Scholar
  13. Tanigaki, N., Tosi, R., Sagawa, K., Minowada, J., and Ferrara, G. B.: The distribution of DR5, MT2, and MB3 specificities on human Ia subsets. Immunogenetics 17: 371–386, 1983bGoogle Scholar
  14. Tanigaki, N., Tosi, R., and Ferrara, G. B.: Serological discrimination of DR4 haplotypes by radioimmunoassay. Immunogenetics 23: 38–46, 1986Google Scholar
  15. Tanigaki, N., Tosi, R., Strominger, J. L., and Cooper, J.: Immunochemistry of the HLA class II molecules isolated from a mouse cell transfected with DQ alpha and beta genes from a DR4 haplotype. Immunogenetics 26: 40–47, 1987Google Scholar
  16. Termijtelen, A. M., Tilanus, M. G. J., Engelen, I., Koning, F., and van Rood, J. J.: Hum. Immunol., in press, 1987Google Scholar
  17. Tosi, R., Tanigaki, N., de Preval, C., Gorski, J., and Mach, B.: Immunochemical analysis of a cell transfected with an HLA-DR gene reveals a new alloantigenic specificity within HLA-DRw52. Eur. J. Immunol. 16: 1603–1608, 1986Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Christine C. Berte
    • 1
  • Nobuyuki Tanigaki
    • 2
  • Roberto Tosi
    • 3
  • Jack Gorski
    • 1
  • Bernard Mach
    • 1
  1. 1.Departement de MicrobiologieUniversite de Geneve, Faculte de MedicineGenevaSwitzerland
  2. 2.Department of Molecular ImmunologyRoswell Park Memorial InstituteBuffaloUSA
  3. 3.Istituto di Biologia Cellulare, C.N.R.RomeItaly

Personalised recommendations