, Volume 27, Issue 3, pp 167–173 | Cite as

Serological recognition of HLA-DR allodeterminant corresponding to DNA sequence involved in gene conversion

  • Christine C. Berte
  • Nobuyuki Tanigaki
  • Roberto Tosi
  • Jack Gorski
  • Bernard Mach


HLA class 11 molecules were isolated from mouse L cells transfected with a DRα gene and an allele, 52a, of locus DR β III from an HLA-homozygous cell line, AVL, of the DR3 haplotype. The isolated molecules were found to possess a new allospecificity, named TR81. This specificity behaved allelic to the previously described DR β III locus. The TR81 specificity was also present on the DR β I gene product of the DR3 haplotype. The nucleotide sequence of the gene encoding TR81 differs from TR81-negative DR β genes of the DRw52 family in only two codons, both located in the regions known to be involved in a gene conversion event. Consequently, the following conclusions can be formulated. (a) TR81 is a bi-locus specificity and allelic to TR22 only in its DR β III locus localization. (b) The TR81 specificity is the phenotypic counterpart of the gene conversion event which led to the generation of the DR β I gene of the DR3 haplotype. (c) One or both individual amino acid substitutions in the first domain of the DR β chain are responsible for the TR81 allospecificity. (d) Since TR81 is expressed on the DR β I chain of the DR3 haplotype, it is possible that TR81 and DR3 represent the same serological specificity.


Nucleotide Codon Nucleotide Sequence Gene Product Amino Acid Substitution 
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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Christine C. Berte
    • 1
  • Nobuyuki Tanigaki
    • 2
  • Roberto Tosi
    • 3
  • Jack Gorski
    • 1
  • Bernard Mach
    • 1
  1. 1.Departement de MicrobiologieUniversite de Geneve, Faculte de MedicineGenevaSwitzerland
  2. 2.Department of Molecular ImmunologyRoswell Park Memorial InstituteBuffaloUSA
  3. 3.Istituto di Biologia Cellulare, C.N.R.RomeItaly

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