Marine Biology

, Volume 117, Issue 1, pp 105–112 | Cite as

Genetic population structure of a species' complex of blue mussels (Mytilus spp.)

  • S. K. Sarver
  • D. W. Foltz
Article

Abstract

Blue mussels representing two nominal species (Mytilus trossulus Gould, 1850 and Mytilus galloprovincialis Lamarck, 1819 were collected from 28 intertidal locations along the Pacific coast of the USA in 1990–1991 (total N=1255) and examined for variation at 15 allozyme loci. Twelve samples, mostly from a region of suspected hybridization, were analyzed for variation in seven shell characters. Principal-components analysis of allozyme data revealed three groups based on first principal-component scores, which were identified as M. trossulus, M. galloprovincialis, and hybrids. Canonical discriminant analysis of shell characters was less successful in separating mussels into discrete groups. Each location was characterized for four environmental variables: (1) temperature, (2) salinity, (3) tidal height and (4) degree of exposure to wave action, which were then used as independent variables in a series of multiple-regression analyses, with the proportions of the two species as dependent variables. Temperature and salinity had significant (P<0.05) effects on the macrogeographic distribution of the two species, whereas the effects of height in the tidal zone and degree of wave exposure were not statistically significant. Salinity was found to have a greater influence than temperature on the microgeographic distribution of the two species. M. trossulus was more abundant at locations with lower temperatures and greater salinity variation than M. galloprovincialis. The two species appear to be ecologically distinct, and their genetic integrity is at least partly the result of environmental heterogeneity.

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Literature cited

  1. Alston, R. E., Turner, B. L. (1963). Natural hybridization among four species of Baptisia (Leguminosae). Am. J. Bot. 50: 159–173Google Scholar
  2. Barrett, E. M. (1963). The California oyster industry. Calif. Fish Game Fish Bull. 123: 1–103Google Scholar
  3. Beaumont, A. R., Seed, R., Garcia-Martinez, P. (1989). Electrophoretic and morphometric criteria for the identification of the mussels Mytilus edulis and M. galloprovincialis. Proc. 23rd Eur. mar. Biol. Symp. 1: 251–258 [Ryland, J. S., Tyler, P. A. (eds.) Olsen & Olsen, Fredensborg, Denmark]Google Scholar
  4. Briggs, B. G. (1962). Interspecific hybridization in the Rununculus lappaceus group. Evolution 16: 372–390Google Scholar
  5. Briggs, J. C. (1974). Marine zoogeography. McGraw-Hill, New York, N.Y.Google Scholar
  6. Campton, D. E. (1987). Natural hybridization and introgression in fishes: methods of detection and genetic interpretations. In: Ryman, N., Utter, F. (eds.) Population genetics and fishery management. University of Washington Press, Seattle, Washington, p. 161–192Google Scholar
  7. Churgin, J. (1974). Temperature, salinity, oxygen, and phosphate in waters off United States. United States Department of Commerce, National Oceanic and Atmospheric Administration Environmental Data Service, National Oceanographic Data Center, Washington, D.C.Google Scholar
  8. Curtis, F. (1966). Molluscan species from early southern California archeological sites. Bull. Sth. Calif. Acad. Sci. 65: 107–127Google Scholar
  9. Dedini, L. A., Schemel, L. E., Turnbull, M. A. (1981). Salinity and temperature measurements in San Francisco Bay waters, 1980. United States Geological Survey, Menlo Park, California (Open-File Rep. No. 82-125)Google Scholar
  10. Gardner, J. P. A. (1992). Mytilus galloprovincialis (Lmk.) (Bivalvia, Mollusca): the taxonomic status of the Mediterranean mussel. Ophelia 35: 219–243Google Scholar
  11. Garthwaite, R. (1986). The genetics of California populations of Geukensia demissa (Dillwyn) (Mollusca): further evidence on the selective importance of leucine aminopeptidase variation in salinity acclimation. Biol. J. Linn. Soc. 28: 343–358Google Scholar
  12. Gartside, D. F. (1980). Analysis of a hybrid zone between chorus frogs of the Pseudoacris nigrita complex in the southern United States. Copeia 1980: 56–66Google Scholar
  13. Gosling, E. M. (1984). The systematic status of Mytilus galloprovincialis in western Europe: a review. Malacologia 25: 551–568Google Scholar
  14. Harger, J. R. (1970). The effect of species composition on the survival of mixed populations of the sea mussels Mytilus californianus and Mytilus edulis. Veliger 13: 147–152Google Scholar
  15. Harrison, R. G., Rand, D. M. (1989). Mosaic hybrid zones and the nature of species boundaries. In: Otte, D., Endler, J. A. (eds.) Speciation and its consequences. Sinauer, Sunderland, Massachusetts, p. 111–133Google Scholar
  16. Haydock, C. I. (1964). An experimental study to control oyster drills in Tomales Bay California. Calif. Fish Game 50: 11–28Google Scholar
  17. Howard, D. J. (1986). A zone of overlap and hybridization between two ground cricket species. Evolution 40: 34–43Google Scholar
  18. Johannesson, K., Kautsky, N., Tedengren, M. (1990). Genotypic and phenotypic differences between Baltic and North Sea populations of Mytilus edulis evaluated through reciprocal transplantations. II. Genetic variation. Mar. Ecol. Prog. Ser. 59: 211–219Google Scholar
  19. Koehn, R. K. (1991). The genetics and taxonomy of species in the genus Mytilus. Aquaculture, Amsterdam 94: 125–145Google Scholar
  20. Koehn, R. K., Hall, J. G., Innes, D. J., Zera, A. J. (1984). Genetic differentiation of Mytilus edulis in eastern North America. Mar. Biol. 79: 117–126Google Scholar
  21. Lewis, J. R., Seed, R. (1969). Morphological variations in Mytilus from southwest England in relation to the occurrence of M. galloprovincialis Lamarck. Cah. Biol. mar. 10: 231–253Google Scholar
  22. Liu, L. L., Foltz, D. W., Stickle, W. B. (1991). Genetic population structure of the southern oyster drill Stramonita (=Thais) haemastoma. Mar. Biol. 111: 71–79Google Scholar
  23. Machell, J. R., DeMartini, J. D. (1971). An annual reproductive cycle of the gaper clam Tresus capax (Gould) in South Humboldt Bay, California. Calif. Fish Game 57: 274–282Google Scholar
  24. Malachowski, M. (1988). The reproductive cycle of the rock scallop Hinnites giganteus (Grey) in Humboldt Bay, California. J. Shellfish Res. 7: 341–348Google Scholar
  25. McDonald, J. H., Koehn, R. K. (1988). The mussels Mytilus galloprovincialis and M. trossulus on the Pacific coast of North America. Mar. Biol. 99: 111–118Google Scholar
  26. McDonald, J. H., Seed, R., Koehn, R. K. (1991). Allozymes and morphometric characters of three species of Mytilus in the Northern and Southern Hemispheres. Mar. Biol. 111: 323–333Google Scholar
  27. Murphy, R. W., Sites, J. W., Buth, D. G., Haufler, C. H. (1990). Proteins I: Isozyme electrophoresis. In: Hillis, D. M., Moritz, C. (eds.) Molecular systematics. Sinauer, Sunderland, Massachusetts, p. 45–126Google Scholar
  28. Nybakken, J., Caillet, G., Broenkow, W. (1977). Ecological and hydrographic studies of Elkhorn Slough, Moss Landing Harbor and nearshore waters. Moss Landing Marine Laboratories, Moss Landing, CaliforniaGoogle Scholar
  29. Richardson, B. J., Baverstock, P. R., Adams, M. (1986). Allozyme electrophoresis: a handbook for animal systematics and population studies. Academic Press, Orlando, FloridaGoogle Scholar
  30. Sarver, S. K. (1989). Genetic differentiation of the mussel Mytilus edulis along the coast of California and in Humboldt Bay. M. S. thesis. Humboldt State University, Arcata, CaliforniaGoogle Scholar
  31. Sarver, S. K. (1993). Genetic population structure of a species complex of blue mussels (Mytilus spp.) Ph. D. dissertation. Louisiana State University, Baton Rouge, LouisianaGoogle Scholar
  32. Sarver, S. K., Landrum, M. C., Foltz, D. W. (1992). Genetics and taxonomy of ribbed mussels (Geukensia spp.). Mar. Biol. 113: 385–390Google Scholar
  33. Sarver, S. K., Loudenslager, E. J. (1991). The genetics of California populations of the blue mussel: further evidence for the existence of electrophoretically distinguishable species or subspecies. Biochem. Syst. Ecol. 19: 183–188Google Scholar
  34. SAS Institute, Inc. (1985). SAS user's guide: statistics. Version 5 edn. SAS Institute, Inc., Cary, North CarolinaGoogle Scholar
  35. Seed, R. (1968). Factors influencing shell shape in the mussel Mytilus edulis. J. mar. biol. Ass. U.K. 48: 561–584Google Scholar
  36. Seed, R. (1972). Morphological variations in Mytilus from the French coasts in relation to the occurrence and distribution of M. galloprovincialis Lamarck. Cah. Biol. mar. 13: 357–384Google Scholar
  37. Seed, R. (1974). Morphological variations in Mytilus from the Irish coasts in relation to the occurrence and distribution of M. galloprovincialis Lamarck. Cah. Biol. mar. 15: 1–25Google Scholar
  38. Seed, R. (1976). Ecology. In: Bayne, B. L. (ed.) Marine mussels: their ecology and physiology. International Biological Programme. 10. Cambridge University Press, Cambridge, England, p. 13–65Google Scholar
  39. Seed, R. (1992). Systematics, evolution and distribution of mussels belonging to the genus Mytilus: an overview. Am. malac. Bull. 9: 123–137Google Scholar
  40. Shaklee, J. B., Allendorf, F. W., Morizot, D. C., Whitt, G. S. (1990). Gene nomenclature for protein-coding loci in fish. Trans. Am. Fish. Soc. 119: 2–15Google Scholar
  41. Shaw, E., Allen, J., Stone, R. (1974). Notes on the collection of shiner perch Cymatogaster aggregata in Bodega Bay, California. Calif. Fish Game 60: 15–22Google Scholar
  42. Skibinski, D. O. F., Beardmore, J. A. (1979). A genetic study of intergradation between Mytilus edulis and M. galloprovincialis. Experientia 35: 1442–1444Google Scholar
  43. Soot-Ryen, T. (1955). A report on the family Mytilidae (Pelecypoda). Allan Hancock Pacif. Exped. 20: 1–175Google Scholar
  44. Tison, J. C. (1967). Surface water temperature and density: Pacific Coast North and South America and Pacific Ocean Islands. Publs U.S. Cst geod. Surv. 31–3: 1–85Google Scholar
  45. Väinölä, R., Hvilsom, M. M. (1991). Genetic divergence and a hybrid zone between Baltic and North Sea Mytilus populations (Mytilidae: Mollusca). Biol. J. Linn. Soc. 43: 127–148Google Scholar
  46. Varvio, S.-L., Koehn, R. K., Väinölä, R. (1988). Evolutionary genetics of the Mytilus edulis complex in the North Atlantic region. Mar. Biol. 98: 51–60Google Scholar
  47. Weir, B. S., Cockerham, C. C. (1984). Estimating F-statistics for the analysis of population structure. Evolution 38: 1358–1370Google Scholar
  48. Wiley, E. O. (1981). Phylogenetics: the theory and practice of phylogenetic systematics. Wiley, New York, N.Y.Google Scholar
  49. Woodruff, D. S., Staub, K. C., Upatham, E. S., Viyanant, V., Yuan, H.-C. (1988). Genetic variation in Oncomelania hupensis: Schistosoma japonicum transmitting snails in China and the Philippines are distinct species. Malacologia 29: 347–361Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • S. K. Sarver
    • 1
  • D. W. Foltz
    • 1
  1. 1.Department of Zoology and PhysiologyLouisiana State UniversityBaton RougeUSA

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