Archives of Toxicology

, Volume 55, Issue 2, pp 91–96 | Cite as

Collagen gel culture system and analysis of estrogen effects on mammary carcinogenesis

  • Satyabrata Nandi
  • Walter Imagawa
  • Yasuhiro Tomooka
  • Michael F. McGrath
  • Marc Edery
Original Investigations


The results obtained to date from studies dealing with the role of hormones, including estrogen, on growth of mammary epithelial cells inside the collagen gel are described. The collagen gel matrix culture system appears to be a suitable system to obtain in vivo-like effects of hormones on mammary cell in vitro. The results thus far indicate that prolactin along with progesterone or cortisol can stimulate mammary cell proliferation. Thus far, estrogen has not been found to be mitogenic in our in vitro system.

Key words

Hormones Mitogen Mammary gland Collagen gel culture Estrogen Cell proliferation 


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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Satyabrata Nandi
    • 1
  • Walter Imagawa
    • 1
  • Yasuhiro Tomooka
    • 1
  • Michael F. McGrath
    • 1
  • Marc Edery
    • 1
  1. 1.Department of Zoology and Cancer Research LaboratoryUniversity of CaliforniaBerkeleyUSA

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