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Oecologia

, Volume 26, Issue 4, pp 363–377 | Cite as

Effects of light and temperature on leaf anatomy and photosynthesis in Fragaria vesca

  • Brain F. Chabot
  • Jean Fincher Chabot
Article

Summary

Fragaria vesca, the woodland strawberry, was grown under a series of controlled environments including variations in light intensity, average temperatures, and temperature amplitude around a constant mean. Observations on CO2 exchange capacities, leaf anatomy, and cell ultrastructure were made for each treatment to determine relationships between these variables. With increasing light intensity, leaf thickness, leaf density, and mesophyll cell surface area and volume per leaf surface area increased. Net photosynthesis (NPS) per leaf weight decreased with increasing light pretreatment while NPS per area increased from low to medium intensity, then decreased at the highest intensity. Depression of photosynthesis at the highest light pretreatment may have been due to massive starch accumulation in the chloroplasts associated with the sodium vapor lamps used. Correlation of all anatomical variables was highly significant with dark respiration and NPS per dry weight but insignificant for NPS per leaf area. In the variable temperature treatments, photosynthetic acclimation occurred with a shift in optimum temperature for NPS in the direction of prevailing growth temperature. Absolute rates were highest at moderate pretreatment temperatures and were reduced by extreme growth temperatures. Thick leaves with low density mesophyll became thinner and more dense with increasing growth temperature corresponding to an increase in maximum net photosynthetic rates. Leaves became thicker and more dense at the highest temperatures, but with an increase in cell damage and indications of changes in metabolic pathways. Highest correlations for gas exchange rates were with specific leaf weight (weight per area). Correlation with other anatomical variables were scattered or insignificant. It was concluded that adaptation to a range of environmental conditions cannot be consistently attributed to changes in mesophyll cell volume or surface area.

Keywords

Mesophyll Cell Leaf Anatomy Leaf Weight Cell Surface Area Leaf Surface Area 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Ballantine, J.E.M., Forde, B.J.: The effect of light intensity and temperature on plant growth and chloroplast ultrastructure in soybean. Amer. J. Bot. 57, 1150–1159 (1970)Google Scholar
  2. Berry, J.A., Fork, D.C., Garrison, S.: Mechanistic studies of thermal damage to leaves. Carnegie Inst. Year Book 74, 751–759 (1975)Google Scholar
  3. Björkman, O.: Further studies on differentiation of photosynthetic properties in sun and shade ecotypes of Solidago virgaurea. Physiol. Plant. 21, 84–99 (1968)Google Scholar
  4. Björkman, O.: Comparative studies on photosynthesis in higher plants, pp. 1–63. In: Photophysiology, Vol. VIII (A.C. Giese, ed.). New York: Academic Press 1973Google Scholar
  5. Blackman, G.E.: Influence of light and temperature on leaf growth, pp. 151–169. In: The growth of leaves (F.L. Milthorpe, ed.). London: Butterworth 1956Google Scholar
  6. Bowes, G., Ogren, W.L., Hageman, R.H.: Light saturation, photosynthesis rate, RuDP carboxylase activity, and specific leaf weight in soybeans grown under different light intensities. Crop Sci. 12, 77–79 (1972)Google Scholar
  7. Chabot, B.F.: Environmental control of photosynthesis and growth of Fragaria vesca. Bull. ecol. Soc. Amer. 56, 45 (1975)Google Scholar
  8. Charles-Edwards, D.A., Charled-Edwards, J., Sant, F.I.: Leaf photosynthetic activity in six temperate grass varieties growth in contrasting light and temperature environments. J. exp. Bot. 25, 715–724 (1974)Google Scholar
  9. Crookston, R.K., Treharne, K.J., Ludford, P., Ozbun, J.L.: Effect of light intensity during growth on the leaf anatomy, photosynthesis and enzyme activity of Phaseolus vulgaris L. Crop Sci. 9, 413–416 (1975)Google Scholar
  10. Cunningham, G.L., Strain, B.R.: Ecological significance of seasonal leaf variability in a desert shrub. Ecology 50, 400–408 (1969)Google Scholar
  11. Dornhoff, G.M., Shibles, R.M.: Varietal differences in net photosynthesis of soybean leaves. Crop. Sci. 10, 42–45 (1970)Google Scholar
  12. Dornhoff, G.M., Shibles, R.M.: Leaf morphology and anatomy in relation to CO2-exchange rate of soybean leaves. Crop Sci. 16, 377–381 (1976)Google Scholar
  13. Drake, B., Raschke, K.: Prechilling of Xanthium strumarium L. reduces net photosynthesis and, independently, stomatal conductance, while sensitizing the stomata to CO2. Plant Physiol. 53, 808–812 (1974)Google Scholar
  14. El-Sharkawy, M.A., Hesketh, J.D.: Photosynthesis among species in relation to characteristics of leaf anatomy and CO2 diffusion resistances. Crop Sci. 5, 517–521 (1965)Google Scholar
  15. Gauhl, E.: Photosynthetic response to varying light intensity in ecotypes of Solanum dulcamara L. from shaded and exposed habitats. Oecologia (Berl.) 22, 275–286 (1976)Google Scholar
  16. Goodchild, D.J., Björkman, O., Pyliotis, N.A.: Chloroplast ultrastructure, leaf anatomy, and content of chlorophyll and soluble protein in rainforest species. Carnegie Inst. Wash. Year Book 71, 102–107 (1972)Google Scholar
  17. Haberlandt, G.: Physiological plant anatomy. London: The Macmillan Company 1914Google Scholar
  18. Hanson, H.C.: Leaf structure as related to environment. Amer. J. Bot. 4, 533–560 (1917)Google Scholar
  19. Holmgren, P.: Leaf factors affecting light-saturated photosynthesis in ecotypes of Solidago virgaurea from exposed and shaded habitats. Physiol. Plant. 21, 676–698 (1968)Google Scholar
  20. Kimball, S.L., Salisbury, F.B.: Ultrastructural changes of plants exposed to low temperatures. Amer. J. Bot. 60, 1028–1033 (1973)Google Scholar
  21. Lar'kina, T.P.: Effect of ecological conditions on structure of wild strawberry leaf. Ekologiya 4, 99–102 (1973)Google Scholar
  22. McCree, K.J., Troughton, J.H.: Prediction of growth rate at different light levels from measured photosynthesis and respiration rates. Plant Physiol. 41, 559–566 (1966)Google Scholar
  23. Mousseau, M.: Les phenomenes de regulation structurale et functionnelle de l'appareil photosynthetique de Teucrium scorodonia: un mecanisme d'adaptation aux conditions d'eclairement. Oecol. Plant. 2, 15–26 (1967)Google Scholar
  24. Nafziger, E.D., Koller, H.R.: Influence of leaf starch concentration on CO2 assimilation in soybean. Plant Physiol. 57, 560–563 (1976)Google Scholar
  25. Neales, T.F., Incoll, L.D.: The control of leaf photosynthesis rate by the level of assimilate concentration in the leaf: a review of the: a review of the hypothesis. Bot. Rev. 34, 107–125 (1968)Google Scholar
  26. Nobel, P.S., Zaragoza, L.J., Smith, W.K.: Relation between mesophyll surface area, photosynthetic rate, and illumination level during development for leaves of Plectranthus parviflorus Henckel. Plant Physiol. 55, 1067–1070 (1975)Google Scholar
  27. Peet, M.M.: Physiological responses of Phaseolus vulgaris L. cultivars to growth environment. Ph. D. Thesis, Cornell Univ., Ithaca, N.Y. (1975)Google Scholar
  28. Pieters, G.A.: The growth of sun and shade leaves of Populus euramericana “Robusta” in relation to age, light intensity and temperature. Med. Land. Wageningen 74, 1–106 (1974)Google Scholar
  29. Sawada, S., Matsushima, H., Miyachi, S.: Effects of growth temperature on photosynthetic carbon metabolism in green plants. III. Differences in structure, photosynthetic activities and activities of ribulose diphosphate carboxylase and glycolate oxidase in leaves of wheat grown under varied temperatures. Plant Cell Physiol 15, 239–248 (1974)Google Scholar
  30. Sitte, H.: Morphometrische Untersuchungen and Zellen, pp. 167–198. In: Quantitative methods in morphology (E. Weibel, H. Elias, eds.) Berlin-Heidelberg-New York: Springer 1967Google Scholar
  31. Taylor, A.O., Craig, A.S.: Plants under climatic stress. II. Low temperature, high light effects on chloroplasts ultrastructure. Plant Physiol 47, 719–725 (1971)Google Scholar
  32. Turrell, F.M.: The area of the internal exposed surface of dicotyledon leaves. Amer. J. Bot. 23, 255–264 (1936)Google Scholar
  33. Warington, I., Peet, M., Patterson, D., Bunce, J., Hellmers, H.: Physiological response of soybean to thermoperiod in relation to growth. Abstr., Plant Physiol., Suppl. p. 105 (1976)Google Scholar
  34. Weibel, E.R.: Stereological principles for morphometry in electron microscope cytology. Int. Rev. Cytol. 26, 235–302 (1969)Google Scholar
  35. Willmot, A., Moore, P.D.: Adaptation to light intensity in Silene alba and S. dioica. Oikos 24, 458–464 (1973)Google Scholar
  36. Wilson, D., Cooper, J.P.: Effect of light intensity during growth on leaf anatomy and subsequent light-saturated photosynthesis among contrasting Lolium genotypes. New Phytol. 68, 1125–1135 (1969)Google Scholar
  37. Woledge, J., Jewiss, O.R.: The effect of temperature during growth on the subsequent rate of photosynthesis in leaves of tall fescue (Festuca arundinacea Schreb.). Ann. Bot. 33, 897–913 (1969)Google Scholar
  38. Yocum, C.S., Lommen, P.W.: Mesophyll resistances, pp. 45–54, In: Perspectives of biophysical ecology (D.M. Gates, R.B. Schmerl, eds.). Berlin-Heidelberg-New York: Springer 1975Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • Brain F. Chabot
    • 1
  • Jean Fincher Chabot
    • 1
  1. 1.Section of Ecology and SystematicsCornell UniversityIthacaUSA

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