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Immunogenetics

, Volume 19, Issue 4, pp 269–277 | Cite as

“Natural” chicken antibodies to red blood cells are mainly directed against the B-G antigen, and their occurrence is independent of spontaneous autoimmune thyroiditis

  • Nikolaus Neu
  • Karel Hála
  • Georg Wick
Article

Abstract

Using an indirect hemagglutination assay we tested sera from 94 healthy normal White Leghorn (NWL) and 117 Obese strain (OS) chickens with spontaneous autoimmune thyroiditis for the presence of “natural” antibodies against major histocompatibility complex (MHC) antigens expressed on red blood cells (RBC). In both groups older animals had a significantly increased frequency of such antibodies, but the titer was age-independent. OS chickens showed almost the same frequency of natural antibodies as NWL, and a comparison between OS birds with high antithyroglobulin autoantibody (Tg-AAb) titers and those with low titers also did not reveal a significant difference. We conclude that the occurrence of natural antibodies has no relation to Tg-AAb. The specificity of natural antibodies for MHC-encoded antigens was investigated in indirect immunofluorescence tests including absorption experiments with RBC and white blood cells (WBC). This analysis revealed that of 14 MHC-specific sera 13 were reacting with the B-G antigen, which is present on RBC only. One serum reacted with the B-F antigen, expressed on all somatic cells, i. e., both on RBC and WBC.

Keywords

Major Histocompatibility Complex White Blood Cell Somatic Cell Thyroiditis Indirect Immunofluorescence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Nikolaus Neu
    • 1
  • Karel Hála
    • 1
  • Georg Wick
    • 1
  1. 1.Institute for General and Experimental PathologyUniversity of Innsbruck, Medical SchoolInnsbruckAustria

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