, Volume 103, Issue 10, pp 708–714 | Cite as

Organisation and origin of a B chromosome centromeric sequence from Brachycome dichromosomatica

  • Carolyn R. Leach
  • Tamzin M. Donald
  • Tricia K. Franks
  • Sabba S. Spiniello
  • Catherine F. Hanrahan
  • Jeremy N. Timmis
Original Articles


Brachycome dichromosomatica is an Australian native daisy that has two pairs of A chromosomes and up to three B chromosomes in some populations. A putative B-specific tandem repeat DNA sequence (Bd49) was isolated previously. Here we describe further characterisation of this sequence and investigate its possible origin. Southern analysis showed that all individual B chromosomes examined have highly methylated tandem repeats of Bd49 but differences in banding pattern for distinct B isolates suggested that the sequence is in a state of flux. Using in situ hybridisation, the sequence was shown to be located at the centromeric region of the B chromosome. Southern analysis of genomic DNA with Bd49 demonstrated that multiple copies of the sequence exist in the genomes of B. eriogona, B. ciliaris, B. segmentosa and B. multifida (none of which have B chromosomes) whereas other species tested (including 0B plants of B. dichromosomatica and 0B B. curvicarpa and B. dentata) have few or no copies. Genomic clones and Bd49-like sequences derived by the polymerase chain reaction (PCR) were obtained from five species but determination of phylogenetic relationships within the genus and inference as to the possible origin of the B chromosome were problematic because of extensive intragenomic heterogeneity of the sequences.


Methylated Polymerase Chain Reaction Developmental Biology Phylogenetic Relationship Tandem Repeat 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Alfenito MR, Birchler JA (1993) Molecular characterization of a maize B chromosome centric sequence. Genetics 135: 589–597Google Scholar
  2. Beukeboom LW, Werren JH (1993) Deletion analysis of the selfish B chromosome Paternal Sex Ration (PSR) in the parasitic wasp Nasonia vitripennis. Genetics 133: 637–648Google Scholar
  3. Blunden R, Wilkes TJ, Forster JW, Jimenez MM, Sandery MJ, Karp A, Jones RN (1993) Identification of the E3900 family, a second family of rye B chromosome specific repeated sequences. Genome 36: 106–711Google Scholar
  4. Carlson WR (1986) The B chromosome of maize. CRC Crit Rev Plant Sci 3: 201–226Google Scholar
  5. Carter CR, Smith-White SW (1972) The cytology of Brachycome lineariloba. 3. Accessory chromosomes. Chromosoma 39: 361–379Google Scholar
  6. Charlesworth B, Sniegowski P, Stephan W (1994) The evolutionary dynamics of repetitive DNA in eukaryotes. Nature 371: 215–220Google Scholar
  7. Cuadrado A, Jouve N (1994) Highly repetitive sequences in B chromosomes of Secale cereale revealed by fluorescence in situ hybridization. Genome 37: 709–712Google Scholar
  8. Diaz MO, Barsacci-Pilone G, Mahon KA, Gall JG (1981) Transcripts from both strands of a satellite DNA occur on lampbrush chromosome loops of the newt Notophthalmus. Cell 24: 649–659Google Scholar
  9. Donald TM, Leach CR, Clough A, Timmis JN (1995) Ribosomal RNA genes and the B chromosome of Brachycome dichromosomatica. Heredity 74: 556–561Google Scholar
  10. Eickbush DG, Eickbush TH, Werren JH (1992) Molecular characterization of repetitive DNA sequences from a B chromosome. Chromosoma 101: 575–583Google Scholar
  11. Epstein LM, Mahon KA, Gall JG (1986) Transcription of a satellite DNA in the newt. J Cell Biol 103: 1137–1144Google Scholar
  12. Finnegan EJ, Brettell RIS, Dennis ES (1993) The role of DNA methylation in the regulation of plant gene expression. In: Jost JP, Saluz HP (eds) DNA methylation: molecular biology and biological significance. Birkhäuser, Boston, pp 218–261Google Scholar
  13. Grunstein M, Hogness D (1975) Colony hybridization: A method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci USA 72: 3961–3965Google Scholar
  14. John UP, Leach CR, Timmis JN (1991) A sequence specific to B chromosomes of Brachycome dichromosomatica. Genome 34: 739–744Google Scholar
  15. Jones RN, Rees H (1982) B chromosomes. Academic Press, LondonGoogle Scholar
  16. Lapitan NLV (1991) Organization and evolution of higher plant nuclear genomes. Genome 35: 171–179Google Scholar
  17. López-León MD, Neves N, Schwarzacher T, Heslop-Harrison JS, Hewitt GM, Camacho J-PM (1994) Possible origin of a B chromosome deduced from its DNA composition using double FISH technique. Chromosome Res 2: 87–92Google Scholar
  18. McQuade LR, Hill RJ, Francis D (1994) B-chromosome systems in the greater glider, Petauroides volans (Marsupialia: Pseudocheiridae). II. Investigation of B-chromosome DNA sequences isolated by micromanipulation and PCR. Cytogenet Cell Genet 66: 155–161Google Scholar
  19. Nur U, Werren JH, Eickbush DG, Burke WD, Eikbush H (1988) A “selfish” B chromosome that enhances its transmission by eliminating the paternal genome. Science 240: 512–513Google Scholar
  20. Palmgren G, Mattsson O, Okkels FT (1991) Specific levels of DNA methylation in various tissues, cell lines, and cell types of Daucus carota. Plant Physiol 95: 174–178Google Scholar
  21. Plowman AB, Bougourd SM (1994) Selectively advantageous effects of B chromosomes on germination behaviour in Allium schoenoprasum L. Heredity 72: 587–593Google Scholar
  22. Reed KM, Beukeboom LW, Eickbush DG, Werren JH (1994) Junctions between repetitive DNAs on the PSR chromosome of Nasonia vitripennis: association of palindromes with recombination. J Mol Evol 38: 352–362Google Scholar
  23. Rhoades MM (1968) Studies on the cytological basis of crossing over. In: Peacock WJ, Brock RD (eds) Replication and Recombination of genetic material. Australian Academy of Science, Canberra, pp 229–241Google Scholar
  24. Sandery MJ, Forster JW, Blunden R, Jones RN (1990) Identification of a family of repeated sequences on the rye B chromosome. Genome 33: 908–913Google Scholar
  25. Sapre B, Deshpande S (1987) Origin of B chromosomes in Coix L through spontaneous interspecific hybridization. J Hered 78: 191–196Google Scholar
  26. Scott NS, Possingham JV (1980) Chloroplast DNA in expanding spinach leaves. J Exp Bot 123: 1081–1092Google Scholar
  27. Smith-White S, Carter CR (1981) The maintenance of B chromosomes in Brachycome dichromosomatica. In: Atchley WR, Woodruff D (eds) Evolution and speciation. Cambridge University Press, London, pp 335–355Google Scholar
  28. Steele Scott N, Kavanagh TA, Timmis JN (1984) Methylation of rRNA genes in some higher plants. Plant Sci Lett 85: 213–217Google Scholar
  29. Tsujimoto H, Niwa K (1992) DNA structure of the B chromosome of rye revealed by in situ hybridization using repetitive sequences. Jpn J Genet 67: 233–241Google Scholar
  30. Watanabe K, Carter CR, Smith-White S (1975) The cytology of Brachycome lineariloba. 5. Chromosome relationships and phylogeny of the race A cytodemes (n=2). Chromosoma 52: 383–397Google Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Carolyn R. Leach
    • 1
  • Tamzin M. Donald
    • 1
  • Tricia K. Franks
    • 1
  • Sabba S. Spiniello
    • 1
  • Catherine F. Hanrahan
    • 1
  • Jeremy N. Timmis
    • 1
  1. 1.Department of GeneticsThe University of AdelaideAdelaideAustralia

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