Molecular and General Genetics MGG

, Volume 136, Issue 4, pp 351–360 | Cite as

Regulation of gene expression in Salmonella phage P22

II. Regulation of expression of late functions
  • Hermann H. Prell
Article
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Summary

Transactivation experiments were performed involving the genetically related Salmonella phages P22, L and Px 1 in order to find out if more than one positively acting regulatory product is engaged in the expression of vegetative gene functions of each of these phages. The results obtained with Px 1 - and L-lysogenic cells superinfected with P22 suggest the following conclusions:
  1. 1.

    The expression of the early genes 12 and 23 and of the late gene 19 (lysozyme synthesis) is positively regulated by two different regulatory products, since P22 transactivates in prophage Px 1 both early and late genes (Prell, 1973), in prophage L only late genes.

     
  2. 2.

    The transactivation by P22 of the lysozyme gene of prophage L takes place in the presence of L repressor. This conclusion is suggested, since the superinfecting P22 does not derepress early gene expression (see 1.), and is confirmed by demonstration of replication inhibition for L phage in L lysogenic cells doubly superinfected with L and P22 phages (Thomas-Bertani-experiment).

     
  3. 3.

    The late gene regulatory protein seems to be synthesized by gene 23, as transactivation experimetns with both L- and Px 1 , prophages suggest.

     
  4. 4.

    The expression of gene 23 itself is turned on by an early regulatory product. The gene which codes for it is still unidentified. However its product seems to by highly specific, since it is active on Px 1 - but not on L-prophage.

     

Keywords

Gene Expression Gene Function Lysozyme Regulatory Protein Late Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • Hermann H. Prell
    • 1
  1. 1.Abteilung für Molekulare Genetik im Institut für Mikrobiologie der Gesellschaft für Strahlen- und Umweltforschung mbH München in GöttingenGerman Federal Republic

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