Duration of the cell division cycle and its dependence on temperature

  • J. F. López-Sáez
  • G. Giménez-Martin
  • A. Gonzalez-Fernández
Article
Received:

Summary

Measurement of the B-interphase has afforded a means of calculating the duration of the cell division cycle at various temperatures, from 0° to 40° C, in the root meristems of Allium cepa. For this purpose caffeine labelling was used to produce a small population (1–5%) of highly synchronized binucleate cells. The B-interphase is what we call the period from 0 hour (end of the one-hour caffeine treatment) to the appearance of the first binucleate cells in bi-prophase. The duration of the phases that make up the cycle was calculated from their relative frequency within the meristematic population.

Observation shows that at 0° and at 40° the cells do not go through the cycle at all and that from 10° to 30° the speed increases in almost linear proportion. There is no significant change in the relative duration of any of the morphological phases under any of the conditions tested.

In discussing these results the authors propose the use of the cell flow (percentage of meristematic cells passing through any point in the cycle in one hour) as an expression in the simplest form of the steady-state kinetics of the meristematic population at a given temperature.

Keywords

Caffeine Cell Division Relative Frequency Simple Form Small Population 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. Agrell, I.: The thermal dependence of the mitotic stages during the early embryonic development of the sea urchin embryo. Arch. Zool. (Stockh.) 11, 383–392 (1958).Google Scholar
  2. Bajer, A., and J. Mole-Bajer: Endosperm; material for study on the physiology of cell division. Acta. Soc. Bot. Pol. 23, 69–110 (1954).Google Scholar
  3. Brown, R. J.: The effects of temperature on the durations of the different stages of cell division in the root-tip. Exp. Bot. 2, 96–110 (1951).Google Scholar
  4. Edwards, J. L., A. L. Koch, P. Youcis, H. L. Freese, M. B. Laite, and J. T. Donalson: Some characteristics of DNA synthesis and the mitotic cycle in Ehrlich ascites tumor cells. J. biophys. biochem. Cytol. 7, 273–282 (1960).Google Scholar
  5. Ephrussi, B.: Sur les coefficients de température des différentes phases de la mitose des oeufs d'oursin (Paracentrotus lividus Lk.) et de l'ascaris megalocephala. Protoplasma (Wien) 1, 105–123 (1927).Google Scholar
  6. Evans, H. J., and J. R. K. Savage: The effect of temperature on mitosis and on the action of colchicine in root meristem cells of Vicia faba. Exp. Cell Res. 18, 51–61 (1959).Google Scholar
  7. Giménez-Martin, G., A. González-Fernández, and J. F. López-Sáez: A new method of labelling cells. J. Cell Biol. 26, 305–309 (1965).Google Scholar
  8. - - - Duration of the division cycle in mononucleate, binucleate and tetraploid cells. Exp. Cell Res. (in press).Google Scholar
  9. González-Fernández, A., J. F. López-Sáez, and G. Giménez-Martin: Inhibition of cytokinesis: bimitosis and polymitosis. Fiton 21, 157–165 (1964).Google Scholar
  10. - - - Duration of the division cycle in mononucleate and binucleate cells. Exp. Cell Res. (in press).Google Scholar
  11. Hejnowicz, Z.: Growth and cell division in the apical meristem of wheat roots. Physiol. Plantarum (Kbh.) 12, 124–138 (1959).Google Scholar
  12. Hoffman, J. G.: Theory of the mitotic index and its application to tissue growth measurement Bull. Math. Biophysics 11, 139–144 (1949).Google Scholar
  13. Hughes, A.: The mitotic cycle, p. 89. London: Butterworths Sci. Publ. 1952.Google Scholar
  14. Kihlman, B.: Chromosome breakage in allium by 8-ethoxycaffeine and X-rays. Exp. Cell Res. 8, 345–368 (1955).Google Scholar
  15. López-Sáez, J. F., and E. Fernández-Gómez: Mitotic partial index and phase indices. Experientia (Basel) 21, 591–592 (1965).Google Scholar
  16. —, M. C. Risueño, and G. Giménez-Martin: Inhibition of cytokinesis in plant cells. J. Ultrastruct. Res. 14, 85–92 (1966).Google Scholar
  17. Tjio, J. H., and A. Levan: The use of oxiquinoline in chromosome analysis. Ann. Estac. Exptl. Aula Dei 2, 21–64 (1950).Google Scholar
  18. Van't Hoff, J., and A. H. Sparrow: The effect of mitotic cycle duration on chromosome breakage in meristematic cells of Pisum sativum. Proc. nat. Acad. Sci. 50, 855–860 (1963).Google Scholar
  19. —, G. B. Wilson, and A. Colon: Studies on the control of mitotic activity: the use colchicine in the tagging of a synchronous population of cells in the meristem of Pisum sativum. Chromosoma (Berl.) 11, 313–321 (1960).Google Scholar
  20. —, and W. K. Ying: Relationship between the duration of the mitotic cycle, the rate of cell production and the rate of growth of Pisum roots at different temperatures. Cytologia (Tokyo) 29, 399–406 (1964).Google Scholar
  21. Wimber, D.: Duration of the nuclear cycle in Tradescantia paludosa root tips as measured with H3-thymidine. Amer. J. Bot. 47, 828–834 (1960).Google Scholar

Copyright information

© Springer-Verlag 1966

Authors and Affiliations

  • J. F. López-Sáez
    • 1
  • G. Giménez-Martin
    • 1
  • A. Gonzalez-Fernández
    • 1
  1. 1.Seccion de Citología, Instituto de Biología CelularC.S.I.C.MadridSpain

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