Advertisement

Current Genetics

, Volume 16, Issue 5–6, pp 347–350 | Cite as

Two genes encode 7SL RNAs in the yeast Yarrowia lipolytica

  • F. He
  • J. M. Beckerich
  • V. Ribes
  • D. Tollervey
  • C. M. Gaillardin
Original Articles

Summary

We have identified an abundant cytoplasmic 7S RNA in crude extracts of the yeast Yarrowia lipolytica. A cDNA probe was prepared from this RNA and used to screen a genomic library. The DNA sequence of a positive clone was determined and the end positions of the 7S RNA gene established by comparison with the sequence of the extremities of 7S RNA. This gene, designated SCR2, encodes a 270-nucleotide RNA that can be folded into a secondary structure similar to that of 7SL RNAs. This RNA is 94.4% homologous to a previously identified 7S RNA from this yeast, but is encoded by a separate gene with highly divergent flanking sequences.

Key words

Yeast 7SL RNA Yarrowia lipolytica 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bark C, Weller P, Zabielski J, Janson L, Pettersson U (1987) Nature 328:356–360Google Scholar
  2. Brennwald P, Liao XB, Holm K, Porter G, Wise JA (1988) Mol Cell Biol 8:1580–1590Google Scholar
  3. Davidow LS, Kaczmarek FS, DeZeeuw JR, Conlon SW, Lauth MR, Pereira DA, Franke AE (1987a) Curr Genet 11:377–383Google Scholar
  4. Davidow LS, O'Donnell MM, Kaczmarek FS, Pereira DA, DeZeeuw JR, Franke AE (1987b) J Bacteriol 169:4621–4629Google Scholar
  5. Kanehisa M (1984) Nucleic Acids Res 12:203–215Google Scholar
  6. Murphy S, Di Liegro C, Melli M (1987) Cell 51:81–87Google Scholar
  7. Poritz MA, Siegel V, Hansen W, Walter P (1988) Proc Natl Acad Sci USA 85:4315–4319Google Scholar
  8. Ribes V, Dehoux P, Tollervey D (1988) EMBO J 7:231–237Google Scholar
  9. Sanger F, Nicklen S, Coulson AR (1977) Proc Natl Acad Sci USA 74:5463–5467Google Scholar
  10. Sharp S, DeFranco D, Dingermann T, Farrell P, Söll D (1981) Proc Natl Acad Sci USA 78:6657–6661Google Scholar
  11. Ullu E, Weiner AM (1985) Nature 318:371–374Google Scholar
  12. Walter P, Blobel G (1980) Proc Natl Acad Sci USA 77:7112–7116Google Scholar
  13. Walter P, Blobel G (1982) Nature 299:691–698Google Scholar
  14. Xuan JW, Fournier P, Gaillardin C (1988) Curr Genet 14:15–21Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • F. He
    • 1
  • J. M. Beckerich
    • 1
  • V. Ribes
    • 2
  • D. Tollervey
    • 2
  • C. M. Gaillardin
    • 1
  1. 1.Laboratoire de génétique des microorganismesInstitut National AgronomiqueThiverval GrignonFrance
  2. 2.Institut PasteurParisFrance

Personalised recommendations