L'ultrastructure de l'adénohypophyse du crapaud Bufo bufo L.

I. Identification des types cellulaires et comparaison des résultats obtenus avec deux fixateurs différents
  • Fanny Mira-Moser
Article

Résumé

Les types cellulaires de l'adénohypophyse du Crapaud (Bufo bufo L.) ont été identifiés et étudiés avec deux fixateurs différents. La fixation au tétroxyde d'osmium permet l'identification de cinq types de cellules, alors que celle à la glutaraldéhyde suivie d'une postfixation met en évidence six catégories cellulaires.

Les résultats différents obtenus avec ces deux fixateurs portent essentiellement sur la structure (forme et densité) des granulations; une meilleure conservation des grains de sécrétion est obtenue avec la glutaraldéhyde.

La libération et l'élaboration des produits de sécrétion sont discutées. Le problème de la libération des granules des cellules hypophysaires a été abordé sous un angle nouveau. Il a été observé dans d'autres organes (pancréas endocrine, cellule à gastrine de l'intestin) que les grains de sécrétion subissent une lyse intracytoplasmique sans destruction de la membrane limitante du granule. Il est suggéré que le même processus pourrait aussi se réaliser au niveau de l'hypophyse.

On the ultrastructure of the adenohypophysis in the toad, Bufo bufo L.

I. Identification of cell types and comparison of the results obtained with different fixations

Summary

Two different fixatives have been used to identify the various cell types contained in the anterior pituitary of the toad (Bufo bufo L.) and to investigate their ultrastructure. Five cell types were distinguished after simple osmium tetroxid fixation, whilst glutaraldehyde fixation with postosmification revealed six distinct cell types.

The results obtained by these two techniques differed mainly in the structure (shape and electrondensity) of the secretory granules, which seemed to be better preserved after combined glutaraldehyde-osmium fixation.

The modes of synthesis and release of the secretory products are discussed. The problem of the mode of release of secretory granules from pituitary cells has been envisaged under a new aspect. It has been observed in other organs (endocrine pancreas, gastrin cells of the intestinal wall) that secretory granules may undergo intracytoplasmic lysis without destruction of their limiting membrane. It is suggested that the same process may operate in the pituitary.

Key-Words

Adenohypophysis Bufo bufo Cell Types Influence of Fixatives 

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© Springer-Verlag 1970

Authors and Affiliations

  • Fanny Mira-Moser
    • 1
  1. 1.Institut d'Histologie et d'Embryologie Ecole de Médecine de l'Université de GenèveFrance

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