Molecular and General Genetics MGG

, Volume 214, Issue 1, pp 162–164 | Cite as

PHO85, a negative regulator of the PHO system, is a homolog of the protein kinase gene, CDC28, of Saccharomyces cerevisiae

  • Akio Toh-e
  • Kazuma Tanaka
  • Yukifumi Uesono
  • Reed B. Wickner
Short Communications


The product of the PHO85 gene, which encodes one of the negative regulatory factors of the PHO system in Saccharomyces cerevisiae, shows significant amino acid sequence homology with the CDC28 protein kinase. However, overexpressing PHO85 did not suppress the temperature sensitive phenotype of the cdc28-1 mutation. The nucleotide sequence of the PHO85 gene strongly suggests the presence of an intron near the sequence encoding the N-terminal region.

Key words

CDC28 Phosphate regulation PHO85 Protein kinase Saccharomyces cerevisiae 


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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Akio Toh-e
    • 1
  • Kazuma Tanaka
    • 1
  • Yukifumi Uesono
    • 1
  • Reed B. Wickner
    • 2
  1. 1.Department of Fermentation TechnologyHiroshima UniversityHigashihiroshimaJapan
  2. 2.Laboratory of Biochemical PharmacologyNIDDK, NIHBethesdaUSA

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