Molecular and General Genetics MGG

, Volume 214, Issue 1, pp 11–15 | Cite as

Operon structure of flagellar genes in Salmonella typhimurium

  • Kazuhiro Kutsukake
  • Yoshikazu Ohya
  • Shigeru Yamaguchi
  • Tetsuo Iino


In Salmonella typhimurium, more than 40 genes have been shown to be involved in flagellar formation and function and almost all of them have been assigned to three regions of the chromosome, termed region I, region II, and region III. In the present study, a large number of transposon-insertion mutants in these flagellar genes were isolated using Tn10 and Mud1. The flaV gene was found to be a strong hot spot for Tn10 insertion. Complementation analysis of the polarity effects exerted by the transposon-insertion mutants defined 13 different flagellar operons; 3 in region I, 4 in region II, and 6 in region III. These results are compared with the reported arrangement of the corresponding genes in Escherichia coli.

Key words

Transposon Polar effect Complementation analysis Flagellar genes Flagellar operons 


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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Kazuhiro Kutsukake
    • 1
  • Yoshikazu Ohya
    • 1
  • Shigeru Yamaguchi
    • 2
  • Tetsuo Iino
    • 1
  1. 1.Laboratory of Genetics, Department of Biology, Faculty of ScienceUniversity of TokyoTokyoJapan
  2. 2.Department of Biology, School of EducationWaseda UniversityTokyoJapan

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