Molecular and General Genetics MGG

, Volume 215, Issue 2, pp 326–331 | Cite as

Sequences responsible for the tissue specific promoter activity of a pea legumin gene in tobacco

  • Anil Shirsat
  • Neville Wilford
  • Ronald Croy
  • Donald Boulter


Maturing pea cotyledons accumulate large quantities of storage proteins at a specific time in seed development. To examine the sequences responsible for this regulated expression, a series of deletion mutants of the legA major seed storage protein gene were made and transferred to tobacco using the Bin19 disarmed Agrobacterium vector system. A promoter sequence of 97 bp including the CAAT and TATA boxes was insufficient for expression. Expression was first detected in a construct with 549 bp of upstream flanking sequence which contained the the leg box element, a 28 bp conserved sequence found in the legumintype genes of several legume species. Constructs containing-833 and-1203 bp of promoter sequence significantly increased levels of expression. All expressing constructs preserved seed specificity and temporal regulation. The results indicate that promoter sequences between positions-97 and-549 bp are responsible for promoter activity, seed specificity, and temporal regulation of the pea legA gene. Sequences between positions-549 and-1203 bp appear to function as enhancer-like elements, to increase expression.

Key words

Pea seed protein genes Promoter deletions Transgenic plants Enhancer elements Seed specific expression 


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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Anil Shirsat
    • 1
  • Neville Wilford
    • 1
  • Ronald Croy
    • 1
  • Donald Boulter
    • 1
  1. 1.Department of BotanyUniversity of Durham, Science LaboratoriesDurhamUK

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