Uptake of radioactive thymidine and cytidine by Ehrlich ascites tumor cells in different stages of growth

  • Renato Baserga
Article
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Summary

In Strong A female mice, the Ehrlich ascites tumor inoculated into the peritoneal cavity grows exponentially for the first 7 days with a doubling time of about 36 hours. The tumor enters then into a late stage during which the number of tumor cells in the peritoneal cavity does not increase. The uptake of intraperitoneally injected thymidine decreases from the exponential to the late stage, mostly because of a decrease in the fraction of cells in DNA synthesis. During the exponential phase, the uptake of thymidine is a function of the amount of radioactive thymidine injected per tumor cell, the utilization decreasing with increasing cell dose. The uptake of intraperitoneally injected cytidine decreases slightly with time after inoculation although the fraction of tumor cells in RNA synthesis remains constant.

Keywords

Tumor Cell Late Stage Thymidine Peritoneal Cavity Female Mouse 
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References

  1. Albert, S., R. M. Johnson, and M. S. Cohan: Phosphorus metabolism in resting and pregnancy-stimulated mammary glands and in spontaneous mammary carcinomas of mice. Cancer Res. 11, 772–776 (1951).PubMedGoogle Scholar
  2. Baserga, R.: A study of nucleic acid synthesis in ascites tumor cells by two emulsion radioautography. J. Cell Biol. 12, 633–637 (1962).CrossRefPubMedGoogle Scholar
  3. —: Mitotic cycle of ascites tumor cells. Arch. Path. 75, 156–161 (1963).PubMedGoogle Scholar
  4. —, and W. E. Kisieleski: Comparative studies of the kinetics of cellular proliferation of normal and tumorous tissues with the use of tritiated thymidine. I. Dilution of the label and migration of labeled cells. J. nat. Cancer Inst. 28, 331–339 (1962).PubMedGoogle Scholar
  5. —, and W. E. Kisieleski: Re-utilization of labeled DNA by Ehrlich ascites tumor cells. Arch. ital. Pat. Clin. Tumori 6, 3–13 (1963).Google Scholar
  6. —, and E. Lisco: Duration of DNA synthesis in Ehrlich ascites cells as estimated by double-labeling with C14- and H3-thymidine and autoradiography. J. nat. Cancer Inst. 31, 1559–1571 (1963).PubMedGoogle Scholar
  7. —, and K. Nemeroff: The use of standard slides in semiquantitative radioautography with tritiated compounds. Stain Technol. 38, 111–116 (1963).PubMedGoogle Scholar
  8. —, S. A. Tyler, and W. E. Kisieleski: The kinetics of growth of the Ehrlich tumor. Arch. Path. 76, 9–13 (1963).PubMedGoogle Scholar
  9. Colter, J. S., R. A. Brown, and K. A. O. Ellem: Observations on the use of phenol for the isolation of deoxyribonucleic acid. Biochim. biophys. Acta (Amst.) 55, 31–39 (1962).CrossRefGoogle Scholar
  10. Defendi, V., and L. A. Manson: Analysis of the life-cycle in mammalian cells. Nature (Lond.) 198, 359–361 (1963).Google Scholar
  11. Edwards, J. L., A. L. Koch, P. Youcis, H. L. Freese, M. B. Laite, and J. T. Donalson: Some characteristics of DNA synthesis and the mitotic cycle in Ehrlich ascites tumor cells. J. biophys. biochem. Cytol. 7, 273–282 (1960).PubMedGoogle Scholar
  12. Feinendegen, L. E., V. P. Bond, and R. B. Painter: Studies on the interrelationship of RNA synthesis, DNA synthesis, and precursor pool in human tissue culture cells studied with tritiated pyrimidine nucleosides. Exp. Cell Res. 22, 381–405 (1961).PubMedGoogle Scholar
  13. Fink, R. M., and K. Fink: Relative retention of H3 and C14 labels of nucleosides incorporated into nucleic acids of Neurospora. J. biol. Chem. 237, 2889–2891 (1962).PubMedGoogle Scholar
  14. Fitzgerald, P. J., and K. Vinijchaikul: Nucleic acid metabolism of pancreatic cells as revealed by cytidine-H3 and thymidine-H3. Lab. Invest. 8, 319–328 (1959).PubMedGoogle Scholar
  15. Friedkin, M., D. Tilson, and D. W. Roberts: Studies of deoxyribonucleic acid biosynthesis in embryonic tissues with thymidine-C14. J. biol. Chem. 220, 627–637 (1956).PubMedGoogle Scholar
  16. Fujioka, M., M. Koga, and I. Lieberman: Metabolism of ribonucleic acid after partial hepatectomy. J. biol. Chem. 238, 3401–3406 (1963).PubMedGoogle Scholar
  17. Healy, G. M., L. Siminovitch, R. C. Parker, and A. F. Graham: Conservation of desoxyribonucleic acid phosphorus in animal cells propagated in vitro. Biochim. biophys. Acta (Amst.) 20, 425–426 (1956).CrossRefGoogle Scholar
  18. Howard, A., and S. R. Pelc: Synthesis of desoxyribonucleic acid in normal and irradiated cells and its relation to chromosome breakage. Heredity (Suppl.) 6, 261–273 (1953).Google Scholar
  19. Joftes, D. L., and S. Warren: Simplified liquid emulsion radioautography. J. biol. photogr. Ass. 23, 145–150 (1955).PubMedGoogle Scholar
  20. Johnson, H. A.: Some problems associated with the histological study of cell proliferation kinetics. Cytologia (Tokyo) 26, 32–41 (1961).Google Scholar
  21. Kihara, H. K., M. Amano, and A. Sibatani: Stability of deoxypentose nucleic acid in growing and non-growing livers of young rats. Biochim. biophys. Acta (Amst.) 21, 489–499 (1956).CrossRefGoogle Scholar
  22. Kisieleski, W. E., R. Baserga, and H. Lisco: Tritiated thymidine and the study of tumors. Atompraxis 7, 81–85 (1961).PubMedGoogle Scholar
  23. Klein, G., and L. Revesz: Quantitative studies on the multiplication of neoplastic cells in vivo. I. Growth curves of the Ehrlich and MClM ascites tumors. J. nat. Cancer Inst. 14, 229–277 (1953).PubMedGoogle Scholar
  24. Kleinschmidt, W. J.: The composition of ribonucleic acid and deoxyribonucleic acid of normal and neoplastic tissue. Cancer Res. 19, 966–969 (1959).PubMedGoogle Scholar
  25. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and B. J. Randall: Protein measurement with the Folin phenol reagent. J. biol. Chem. 193, 265–275 (1951).PubMedGoogle Scholar
  26. Maruyama, Y.: An isotopic method for determination of cell generation time. Nature (Lond.) 198, 1181–1183 (1963).Google Scholar
  27. Messier, B., and C. P. Leblond: Preparation of coated radioautographs by dipping sections in fluid emulsion. Proc. Soc. exp. Biol. (N.Y.) 96, 7–10 (1957).Google Scholar
  28. Patt, H. M., and R. L. Straube: Measurement and nature of ascites tumor growth. Ann. N.Y. Acad. Sci. 63, 728–736 (1956).PubMedGoogle Scholar
  29. Petersen, R. O., and R. Baserga: Route of injection and uptake of tritiated precursors. Arch. Path. 77, 582–586 (1964).PubMedGoogle Scholar
  30. Reichard, P., and B. Estborn: Utilization of desoxyribosides in the synthesis of polynucleotides. J. biol. Chem. 188, 839–846 (1951).PubMedGoogle Scholar
  31. Revesz, L., A. Forssberg, and G. Klein: Quantitative studies on the multiplication of neoplastic cells in vivo. III. Metabolic stability of deoxypentose nucleic acid and the use of labeled tumor cells for the measurement of growth curves. J. nat. Cancer Inst. 17, 37–47 (1956).PubMedGoogle Scholar
  32. Scott, J. F., A. P. Fraccastoro, and E. B. Taft: Studies in histochemistry. I. Determination of nucleic acids in microgram amounts of tissue. J. Histochem. Cytochem. 4, 1–10 (1956).PubMedGoogle Scholar
  33. Stillstrom, J.: Grain count corrections in autoradiography. Int. J. appl. Radiat. 14, 113–118 (1963).CrossRefPubMedGoogle Scholar
  34. Taylor, J. H.: Nucleic acid synthesis in relation to the cell division cycle. Ann. N.Y. Acad. Sci. 90, 409–421 (1960).PubMedGoogle Scholar
  35. Ultman, J. E., E. Hirschberg, and A. Gellhorn: The effect of nitrogen mustard on the cellular concentration of nucleic acids in regenerating rat liver. Cancer Res. 13, 14–20 (1953).PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1964

Authors and Affiliations

  • Renato Baserga
    • 1
  1. 1.Department of PathologyNorthwestern University Medical SchoolChicagoUSA

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