Molecular and General Genetics MGG

, Volume 181, Issue 1, pp 20–23 | Cite as

Antigenicity of nitrate reductase-deficient mutants in Hordeum vulgare L.

  • T. Kuo
  • A. Kleinhofs
  • D. Somers
  • R. L. Warner


Ten nitrate reductase (NR)-deficient mutants have been characterized for their cross-reactivity against specific barley (Hordeum vulgare L.) nitrate reductase antibodies. The rabbit antibodies raised against the purified barley wild type (cv. Steptoe) enzyme quantitatively inactivate nitrate reductase in crude extracts. All nitrate-grown (induced) mutants show positive precipitin reaction against the antiserum by Ouchterlony double diffusion test and all have the ability to neutralize antisera in a NR protection assay. Under induced growth conditions, mutants Az 12, Az 23, Az 29 and Az 30 which have low NR associated catalytic activities also have the lowest level of antigenicity; mutants Az 13, Az 31, Az 33 and Az 34 have intermediate level of both NR associated catalytic activities and antigenicity, while mutants Az 28 and Az 32 have the highest level of both NR associated catalytic activities and antigenicity. Under noninduced growth conditions, all mutants except Az 12 contain detectable but very low levels of NR antigenicity. These results support the concept that these NR-deficient mutants with various levels of NR associated catalytic activities represent different mutation events at the loci coding the NR structural components.


Nitrate Reductase Hordeum Vulgare Rabbit Antibody Protection Assay Diffusion Test 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



nitrate reductase




flavin adenine dinucleotide


bovine serum albumin


nitrate reductase cross-reacting materials


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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • T. Kuo
    • 1
  • A. Kleinhofs
    • 1
  • D. Somers
    • 2
  • R. L. Warner
    • 2
  1. 1.Department of Agronomy and Soils and Program in GeneticsWashington State UniversityPullmanUSA
  2. 2.Department of Agronomy and SoilsWashington State UniversityPullmanUSA

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