The development, topographical relations and innervation of the epiphysis cerebri in the albino rat

  • J. Ariëns Kappers


In this paper, a description is given of the development of the epiphysis cerebri of the albino rat and of some structures showing topographical relations with this organ. Furthermore, the innervation of the pineal body is examined in normal adult rats as well as in a number of specimens in which both superior cervical ganglia were removed. The main conclusions can be summarized as follows.
  1. 1.

    The anlage of the epiphysis first appears on the 14th day of embryonic development.

  2. 2.

    The epiphysis develops by follicular proliferation of the wall of the epiphyseal evagination. In the rat, especially the rostro-dorsal part of this wall is involved in the process.

  3. 3.

    Leptomeningeal mesenchyme containing vessels grows in between the pineal follicles. The adult organ shows a dense vascular network. It is a compact structure consisting of cellnests the follicular structure of which can be still distinguished at many places.

  4. 4.

    In embryos, aged 14 days, fibres could be clearly demonstrated in the caudal commissure. The first habenular commissural fibres were shown in embryos of the 16th day.

  5. 5.

    During development the true pineal recess of the third ventricle disappears by fusing of the epiphyseal peduncles. In the adult, a small recess bordered by the intercalary lamina is sometimes present between the habenular and the caudal commissure. This recess of the third ventricle has been termed intercommissural recess.

  6. 6.

    During early postnatal development the pineal body gradually shifts in a caudal and somewhat dorsal direction. In the adult brain it shows a superficial position just in front of the cerebellum between the caudal collicles. Its lateral parts are sometimes covered by the caudal poles of the cerebral hemispheres.

  7. 7.

    The epiphysis is enveloped by a sheath of leptomeningeal origin. The floor of the confluens sinuum covers the dorsal surface of the organ.

  8. 8.

    The pineal body keeps its connexion with the commissural region by means of a thin and slender avascular epiphyseal stalk consisting of pinealocytes, pinealoblasts and fibrocytes. This stalk can be divided topographically in three parts. Dorsal to it either two veins, vena prosencephali mediana and vena cerebri magna, are present or a single large vena cerebri magna. These veins discharge into the confluens sinuum. The vena prosencephali mediana may fuse either phylogenetically or ontogenetically with the vena cerebri magna originally present. This, then, results in the formation of a single large vena cerebri magna draining, a. o., the choroid plexuses of the third as well as those of both lateral ventricles and the basal veins.

  9. 9.

    In the epiphyseal stalk nerve fibres could be constantly demonstrated. Most of the fibres derive from the habenular commissure the contribution of fibres from the caudal commissure being only small. The nerve bundle in the epiphyseal stalk has been termed commissuro-epiphyseal tract.

  10. 10.

    The fibres of the commissuro-epiphyseal tract are aberrant commissural fibres. They are of no functional significance for the innervation of the pinealocytes. They may leave the stalk or the rostral part of the epiphysis passing into the surrounding leptomeningeal tissue. Other fibres, after having entered the pineal organ, perform loops of 180° returning to the commissural region in the epiphyseal stalk. In one case an ending of a commissuro-epiphyseal fibre was seen. This, however, was most probably not a functional terminal. Very rarely, single commissuro-epiphyseal fibres were seen running as far as the caudal part of the organ.

  11. 11.

    The epiphysis shows an extensive autonomic innervation which is principally supplied by two nervi conarii, bilaterally present. These nerve fascicles course in the tentorium cerebelli penetrating into the epiphysis underneath the floor of the confluens sinuum.

  12. 12.

    After removal of both superior cervical ganglia the fibres of the nervi conarii degenerate evidently being postganglionic fibres originating in these ganglia.

  13. 13.

    The terminal autonomic pineal innervation happens by means of interfollicular strands of fibres from which thin single fibres or very small fibre bundles were seen branching and penetrating the follicles or cellnests of pinealocytes, thus running intrafollicularly.

  14. 14.

    Structures being most probably autonomic motor terminals have been observed. They are exclusively related to the pinealocytes, not to the vascular walls.

  15. 15.

    Among the interfollicular fibre strands interstitial cells have been observed. The present author is inclined to share the opinion of investigators holding that these cells are not of a nervous nature but belong to the same category of cells as the lemnocytes do.

  16. 16.

    After removal of both superior cervical ganglia the terminal intrapineal autonomic innervation was seen to disappear completely. In very few preparations only some rare fibres were left. Evidently, pineal innervation is mainly, if not exclusively, orthosympathetic.

  17. 17.

    On the ground of the observations mentioned as well as of the results of recent electronmicroscopical investigations it has been concluded that the interas well as the intrafollicular fibres and fibre strands consist of the thin terminal ramifications of postganglionic nerve fibres originating in the superior cervical ganglia. Proof of the existence of a sensory innervation of the epiphysis has not been found so far. The opinion of some authors, holding that, in general, the terminal autonomic “neurofibrillar network” would be formed by the interstitial cells presumably being synaptically connected with postganglionic fibres is discussed and considered improbable.

  18. 18.

    In the rat, so far, neither nervous nor vascular relations have been observed pointing to the existence of an „epithalamo-epiphyseal-“ or „habenulo-epiphyseal complex“ that, in any way, could be compared with the hypothalamo-hypophyseal complex.



Autonomic Motor Superior Cervical Ganglion Pineal Organ Autonomic Innervation Pineal Body 
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  1. Antonow, A.: Zur Frage von dem Bau der Glandula pinealis. Anat. Anz.60, 21–31 (1925/26).Google Scholar
  2. Bargmann, W.: Die Epiphysis cerebri. In: Handbuch der mikroskopischen Anatomie des Menschen, Bd. VI/4, S. 309–502, herausgeg. Von W.V. Möllendorff. Berlin: Springer 1943.Google Scholar
  3. Bartheld, F.V., and J.Moll: The vascular system of the mouse epiphysis with remarks on the comparative anatomy of the venous trunks in the epiphyseal area. Acta anat. (Basel)22, 227–235 (1954).Google Scholar
  4. Boeke, J.: Innervationsstudien. VI. Der sympathische Grundplexus in seinen Beziehungen zu den Drüsen. Z. mikr.-anat. Forsch.35, 551–601 (1934).Google Scholar
  5. Boeke, J.: The sympathetic endformation, its synaptology, the interstitial cells, the periterminal network, and its bearing on the neurone theory. Discussion and critique. Acta anat. (Basel)8, 18–61 (1949).Google Scholar
  6. Caesar, R.: Elektronenmikroskopische Beobachtungen zum Verhalten der marklosen Nervenfasern im glatten Muskelgewebe. Verh. Anat. Ges., 55. Versamml. April 1958. Erg.-H. Anat. Anz.105, 90–100 (1959).Google Scholar
  7. Caesar, R., G. A.Edwards and H.Ruska: Architecture and nerve supply of smooth muscle tissue. J. Biophys. biochem. Cytol.3, 867–878 (1957).Google Scholar
  8. Cajal, S. R.: Textura del sistema nervioso del hombre y de los Vertebrados, T. II, 2da parte. Madrid: Moya 1904.Google Scholar
  9. Champy, Ch.: Granules et substances réduisants l'iodure d'osmium. J. Anat. (Paris)49, 323–343 (1913).Google Scholar
  10. Champy, C., et Chr.Champy-Coujard: L'étude histochimique des secrétions des neurones. Acta anat. (Basel)30, 169–174 (1957).Google Scholar
  11. Champy, C., R.Coujard et Ch.Coujard-Champy: L'innervation sympathique des glandes. Acta anat. (Basel)1, 233–283 (1945/46).Google Scholar
  12. Champy, C., et S.Hatem: Sur les réactions histochimiques du complex tétroxyde d'osmiumiodure et leurs utilisations. Bull. Micr. appl.5, 93–97 (1955).Google Scholar
  13. Clara, M.: Wo steht die Morphologie der neurovegetativen Peripherie? Acta neuroveg. (Wien) Suppl.6, 1–17 (1955).Google Scholar
  14. Clark, S. L.: Innervation of the pia mater of the spinal cord and medulla. J. comp. Neurol.53, 129–146 (1931).Google Scholar
  15. Cooper, E. R. A.: The innervation of the meninges and the choroid plexuses. Acta anat. (Basel)33, 298–318 (1958).Google Scholar
  16. Coujard, R.: Essais sur la signification chimique de quelques méthodes histologiques. Bull. Histol. Techn. micr.20, 161–173 (1943).Google Scholar
  17. Coujahd, R.: Le rôle trophique du sympathique. C. R. Association des Anatomistes. 41e Réunion. Bull. Assoc. Anat. Nr. 87, 887–983 (1955).Google Scholar
  18. Elfvin, L. G.: The ultrastructure of unmyelinated fibers in the splenic nerve of the cat. J. ultrastruct. Res.1, 428–454 (1958).Google Scholar
  19. Favaro, G.: Le fibre nervose prepineali e pineali nell'encefalo dei mammiferi. Arch. ital. Anat.3, 750–789 (1904).Google Scholar
  20. Fernandez-Moran, H.: Elektronenmikroskopische Untersuchung der Markscheide und des Achsenzylinders im internodalen Abschnitt der Nervenfaser. Experientia (Basel)6, 339 (1950).Google Scholar
  21. Gabrielesco, E., et A.Bordeianu: Recherches sur les réactions morphologiques des terminaisons nerveuses dans les états de stimulation physiologique. Acta anat. (Basel)36, 101–119 (1959).Google Scholar
  22. Gardner, J. H.: Development of the pineal body in the hooded rat. Anat. Rec.103, 538 (abstract) (1949).Google Scholar
  23. Gardner, H. J.: Innervation of the pineal gland in hooded rats. J. comp. Neurol.99, 319–330 (1953).Google Scholar
  24. Gerebtzoff, M. A.: Cholinesterases, a histochemical contribution to the solution of some functional problems. London-New York-Paris-Los Angeles: Pergamon Press 1959.Google Scholar
  25. Greene, E. Ch.: Anatomy of the rat. Trans. Amer. philos. Soc., N. S.17 (1935).Google Scholar
  26. Greving, R.: Die Innervation der Epiphyse. In: Lebensnerven und Lebenstriebe, S. 209–226, herausgeg. Von L. R.Müller. Berlin: Springer 1931.Google Scholar
  27. Gurdjian, E. S.: Olfactory connections in the albino rat, with special reference to the stria medullaris and anterior commissure. J. comp. Neurol.38, 127–163 (1925).Google Scholar
  28. Hartmann, F.: Über die Innervation der Epiphysis cerebri einiger Säugetiere. Z. Zellforsch.46, 416–429 (1957).Google Scholar
  29. Henneberg, B.: Normentafel zur Entwicklungsgeschichte der Wanderratte (Rattus norvegicus Erxleben). Normentafel zur Entwicklungsgeschichte der Wirbeltiere, begründetVon F.Keibel, 15. Erg.-H. Jena: Fischer 1937.Google Scholar
  30. Herring, P. T.: The pineal region of the mammalian brain: its morphology and histology in relation to function. Quart. J. exp. Physiol.17, 125–147 (1927).Google Scholar
  31. Hillarp, N.-Å.: Structure of the synapse and the peripheral innervation apparatus of the autonomic nervous system. Acta anat. (Basel) Suppl.4, 1–153 (1946).Google Scholar
  32. Hillarp, N.-Å.: The construction and functional organization of the autonomic innervation apparatus. Acta physiol. scand.46, Suppl. 157, 1–38 (1959a).Google Scholar
  33. Hillarp, N.-Å.: On the histochemical demonstration of adrenergic nerves with the osmic acid-sodium iodide technique. Acta anat. (Basel)38, 379–384 (1959b).Google Scholar
  34. Hosaka, Y., A.Nakai and S.Kushima: Innervation of the pineal body and the supracommissural and subcommissural organs of the Japanese monkey. Bull. Tokyo Med. Dent. Univ.4, 365–378 (1957).Google Scholar
  35. Jabonero, V.: Études sur le système neurovégétatif périphérique. VI. Les synapses. Acta anat. (Basel)15, 329–392 (1952).Google Scholar
  36. Jabonero, V.: Die interstitiellen Zellen des vegetativen Nervensystems und ihre vermutliche Analogie zu anderen Elementen. I und II. Acta neuroveg. (Wien)5, 1–24, 266–280 (1952/53).Google Scholar
  37. Jabonero, V.: Der anatomische Aufbau des peripheren neurovegetativen Systems. Acta neuroveg. (Wien) Suppl.4, 1–159 (1953).Google Scholar
  38. Jabonero, V.: Le syncytium nerveux distal des voies végétatives efférentes. Acta neuroveg. (Wien)8, 291–324 (1954).Google Scholar
  39. Jabonero, V.: Die anatomischen Grundlagen der peripheren Neurosekretion. Acta neuroveg. (Wien) Suppl.6, 159–302 (1955).Google Scholar
  40. Josephy, H.: Die feinere Histologie der Epiphyse. Z. ges. Neurol. Psychiat.62, 91–119 (1920).Google Scholar
  41. Kolmer, W.: Ganglienzellen als konstanter Bestandteil der Zirbel von Affen. Z. ges. Neurol. Psychiat.121, 423–428 (1929).Google Scholar
  42. Kolmer, W., u. R.Löwy: Beiträge zur Physiologie der Zirbeldrüse. Pflügers Arch. ges. Physiol.196, 1–14 (1922).Google Scholar
  43. Krabbe, K. H.: Quelques considérations sur la glande pinéale et le complexe épithalamoépiphysaire. Rev. neurol.70, 596–603 (1938).Google Scholar
  44. LeGros Clark, W. E.: The nervous and vascular relations of the pineal gland. J. Anat. (Lond.)74, 470–492 (1940).Google Scholar
  45. Levin, P. M.: A nervous structure in the pineal body of the monkey. J. comp. Neurol.68, 405–409 (1938).Google Scholar
  46. Marburg, O.: Zur Kenntnis der normalen und pathologischen Histologie der Zirbeldrüse. Die Adipositas cerebralis. Arb. neurol. Inst. Univ. Wien17, 217–279 (1909).Google Scholar
  47. Mazzucchelli, B.: Osservazioni sulla struttura e sulla innervazione della epifisi cerebrale dei mammiferi. Monit. zool. ital.58, 1–4 (1950).Google Scholar
  48. Meyling, H. A.: Das periphere Nervennetz und sein Zusammenhang mit den ortho- und parasympathischen Nervenfasern. Acta neuroveg. (Wien) Suppl.6, 35–63 (1955).Google Scholar
  49. Nelemans, F. A., and J.Dogterom: Structure and function of the peripheral autonomic nervous system. Acta neuroveg. (Wien) Suppl.6, 101–121 (1955).Google Scholar
  50. Pastori, G.: Über Nervenfasern und Nervenzellen in der „Epiphysis cerebri“. Z. ges. Neurol. Psychiat.117, 202–211 (1928).Google Scholar
  51. Pastori, G.: Ein bis jetzt noch nicht beschriebenes sympathisches Ganglion und dessen Beziehungen zum Nervus conari sowie zur Vena magna Galeni. Z. ges. Neurol. Psychiat.123, 81–90 (1930).Google Scholar
  52. Pines, L.: Über die Innervation der Epiphyse. Z. ges. Neurol. Psychiat.111, 357–369 (1927).Google Scholar
  53. Polvani, F.: Studio anatomico della glandola pineale umana. Fol. Neuro-biol. Haarlem7, 655–695 (1913).Google Scholar
  54. Quay, W. B.: Striated muscle in the mammalian pineal organ. Anat. Rec.133, 57–61 (1958).Google Scholar
  55. Richardson, K. C.: Electronmicroscopic observations on Auerbach's plexus in the rabbit, with special reference to the problem of smooth muscle innervation. Amer. J. Anat.103, 99–136 (1958).Google Scholar
  56. Romeis, B.: Mikroskopische Technik. München: Leibniz 1948.Google Scholar
  57. Roussy, G., et M.Mosinger: Le complexe épithalamo-épiphysaire. Ses corrélations avec le complexe hypothalamo-hypophysaire. Le système neuro-endocrinien du diencéphale. Rev. neurol.69, 459–470 (1938).Google Scholar
  58. Schaltenbrand, G.: Plexus und Meningen. In: Handbuch der mikroskopischen Anatomie des Menschen, Bd. IV/2, S. 1–139. Begründetvon W. v. Möllendorff, fortgeführtvon W. Bargmann. Berlin-Göttingen-Heidelberg: Springer 1955.Google Scholar
  59. Schultz, R. L., E. A.Maynard and D.Pease: Electron microscopy of neurons and neuroglia of cerebral cortex and corpus callosum. Amer. J. Anat.100, 369–407 (1957).Google Scholar
  60. Stöhr Jr., Ph.: Zusammenfassende Ergebnisse über die Endigungsweise des vegetativen Nervensystems. Acta neuroveg. (Wien)10, 21–109 (1954).Google Scholar
  61. Stöhr Jr., Ph.: Mikroskopische Anatomie des vegetativen Nervensystems. In Handbuch der mikroskopischen Anatomie des Menschen, Bd. IV/5, S. 1–678. Begründetvon W. v. Möllendorff, fortgeführtvon W. Bargmann. Berlin-Göttingen-Heidelberg: Springer 1957.Google Scholar
  62. Szentágothai, J.: Einige Bemerkungen zur Struktur der peripheren Endausbreitung vegetativer Nerven. Acta neuroveg. (Wien)15, 417–445 (1957).Google Scholar
  63. Tubahara, M.: Über die Innervation der Epiphyse. Kurume med. J.2, 218–234 (1955).Google Scholar
  64. Walter, F. K.: Beiträge zur Histologie der menschlichen Zirbeldrüse. Z. ges. Neurol. Psychiat.17, 65–79 (1913).Google Scholar
  65. Walter, F. K.: Zur Histologie und Physiologie der menschlichen Zirbeldrüse. Z. ges. Neurol. Psychiat.74, 314–330 (1922).Google Scholar
  66. Walter, F. K.: Weitere Untersuchungen zur Pathologie und Physiologie der Zirbeldrüse. Z. ges. Neurol. Psychiat.83, 411–463 (1923).Google Scholar

Copyright information

© Springer-Verlag 1960

Authors and Affiliations

  • J. Ariëns Kappers
    • 1
  1. 1.Department of Anatomy and EmbryologyState UniversityGroningenThe Netherlands

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