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Molecular and General Genetics MGG

, Volume 211, Issue 1, pp 183–185 | Cite as

ack::Mu d1-8 (Aprlac) operon fusions of Salmonella typhimurium LT2

  • H. S. Kwan
  • H. W. Chui
  • K. K. Wong
Short Communications

Summary

Several ack::Mu d1-8 insertions were isolated from Salmonella typhimurium on MacConkey-glucose-trimethylamine oxide medium. They could not produce gas from glucose in the absence of exogeneous formate. Two of the mutants expressed β-galactosidase activity and were shown to be ack::Mu d1-8 fusions by genetic analysis. These insertions were characterized and located on the genetic map at the ack locus by co-transduction with zei::Tn10. The gene order zei::Tn10-ack-pta was established. The direction of transcription of ack was clockwise on the genetic map. Expression of the lac operon in ack::Mu d1-8 was increased twofold by anaerobiosis. Addition of formate, pyruvate, and acetate did not affect the anaerobic expression of the lac operon in ack::Mu d1-8.

Key words

Acetate kinase Phosphotransacetylase Anaerobiosis Mu d1-8 fusions 

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References

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • H. S. Kwan
    • 1
  • H. W. Chui
    • 1
  • K. K. Wong
    • 1
  1. 1.Department of BiologyThe Chinese University of Hong KongShatin, N.T.Hong Kong

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