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Molecular and General Genetics MGG

, Volume 211, Issue 1, pp 17–26 | Cite as

Two classes of Rhizobium meliloti infection mutants differ in exopolysaccharide production and in coinoculation properties with nodulation mutants

  • Peter Müller
  • Michael Hynes
  • Dieter Kapp
  • Karsten Niehaus
  • Alfred Pühler
Article

Summary

Symbiotic mutants of Rhizobium meliloti were isolated following Tn5 mutagenesis. Besides four nodulation mutants (Nod-) unable to induce nodule formation on alfalfa, five infection mutants (Inf-), which induce the formation of root nodules without detectable infection threads or bacteroids, were obtained. The Inf- mutants were subdivided into two classes. One class contains mutants which fail to synthesize acidic exopolysaccharide (EPS-). The other class is comprised of mutants which produce excess amounts of acidic exopolysaccharide (EPS*). 13C nuclear magnetic resonance spectroscopy of the exopolysaccharide isolated from one of the latter type of Inf- mutant, 101.45, revealed that the side chain of the repeating octosaccharide unit lacks the terminal pyruvate residue. Complementing cosmids were isolated for all Inf- mutants. In the case of the Inf- EPS- mutants the complementing cosmids contain DNA segments which overlap and are part of megaplasmid 2. For two mutants the mutations were found to map on a 7.8 kb EcoRI fragment. In the case of the Inf- EPS* mutants the complementing cosmids carry chromosomal DNA. The mutations of two Inf- EPS* mutants were localized on a 6.4 kb EcoRI fragment. Coinoculation of alfalfa plants with Nod- and Inf- EPS- mutants resulted in effective symbiosis. The nodules appeared wild type and fixed nitrogen. In constrast, coinoculations with Nod- mutants and the Inf- EPS* mutant 101.45 did not result in the formation of effective nodules.

Key words

Rhizobium meliloli Infection mutants Nodulation mutants 

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Peter Müller
    • 1
  • Michael Hynes
    • 1
  • Dieter Kapp
    • 1
  • Karsten Niehaus
    • 1
  • Alfred Pühler
    • 1
  1. 1.Lehrstuhl für Genetik, Fakultät für BiologieUniversität BielefeldBielefeld 1Federal Republic of Germany

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