Molecular and General Genetics MGG

, Volume 178, Issue 3, pp 583–588 | Cite as

Identification of the E. coli dnaJ gene product

  • C. P. Georgopoulos
  • A. Lundquist-Heil
  • J. Yochem
  • M. Feiss


We have previously shown that a mutation (groPC259) in the E. coli dnaJ gene renders the cell inviable at high temperatures and arrests bacteriophage λ DNA replication at all temperatures (Sunshine et al., 1977). We have isolated λdnaJ++ transducing phages both by in vitro cloning and by abnormal excision of a λdnaK transducing phage integrated near the dnaJ locus. The dnaJ gene product has been identified on SDS polacrylamide gels after infection of UV-irradiated E. coli cells by λdnaJ++ derivative phages. It is a polypeptide chain with an apparent molecular weight of 37,000-daltons. This has been verified by the fact that a transducing phage carrying an amber mutation in the dnaJ gene fails to induce the synthesis of the 37,000-dalton polypeptide chain upon infection of sup++ bacteria, but does so upon infection of supF or supD bacteria.


Molecular Weight Polypeptide Gene Product Polypeptide Chain Apparent Molecular Weight 
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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • C. P. Georgopoulos
    • 1
  • A. Lundquist-Heil
    • 1
  • J. Yochem
    • 2
  • M. Feiss
    • 2
  1. 1.Department of Cellular, Viral and Molecular Biology, College of MedicineUniversity of UtahSalt Lake CityUSA
  2. 2.Department of Microbiology, College of MedicineThe University of IowaIowa CityUSA

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