Advertisement

Molecular and General Genetics MGG

, Volume 178, Issue 3, pp 583–588 | Cite as

Identification of the E. coli dnaJ gene product

  • C. P. Georgopoulos
  • A. Lundquist-Heil
  • J. Yochem
  • M. Feiss
Article

Summary

We have previously shown that a mutation (groPC259) in the E. coli dnaJ gene renders the cell inviable at high temperatures and arrests bacteriophage λ DNA replication at all temperatures (Sunshine et al., 1977). We have isolated λdnaJ++ transducing phages both by in vitro cloning and by abnormal excision of a λdnaK transducing phage integrated near the dnaJ locus. The dnaJ gene product has been identified on SDS polacrylamide gels after infection of UV-irradiated E. coli cells by λdnaJ++ derivative phages. It is a polypeptide chain with an apparent molecular weight of 37,000-daltons. This has been verified by the fact that a transducing phage carrying an amber mutation in the dnaJ gene fails to induce the synthesis of the 37,000-dalton polypeptide chain upon infection of sup++ bacteria, but does so upon infection of supF or supD bacteria.

Keywords

Molecular Weight Polypeptide Gene Product Polypeptide Chain Apparent Molecular Weight 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Casjens, S., Hendrix, R.W.: Locations and amounts of the major structural proteins in bacteriophage lambda. J. Mol. Biol. 90, 20–23 (1974)Google Scholar
  2. Collins, J., Hohn, B.: Cosmids: A type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage λ heads. Proc. Nat. Acad. Sci. USA 75, 4242–4246 (1978)Google Scholar
  3. Degnen, G.E., Cox, E.C.: Conditional mutator gene in Escherichia coli: isolation mapping and effector studies. J. Bact. 117, 477–487 (1974)Google Scholar
  4. Dove, W.F., Hargrove, E., Ohashi, M., Haugli, F., Guha, A.: Replicator activation in lambda. Japan J. Genet. 44, suppl. 1, 11–22 (1969)Google Scholar
  5. Dove, W.F., Inokuchi, H., Stevens, W.F.: Replication control in phage lambda. In: The bacteriophage lambda (A.D. Hershey, ed.), pp. 747–772. New York: Cold Spring Harbor Laboratory 1971Google Scholar
  6. Fowler, R.G., Degnen, G.E., Cox, E.C.: Mutational specificity of a conditional Escherichia coli mutator mutD5. Mol. Gen. Genet. 133, 179–191 (1974)Google Scholar
  7. Furth, M.E., McLeester, C., Dove, W.F.: Specificity determinants for bacteriophage lambda DNA replication. I. A chain of interactions that controls the initiation. J. Mol. Biol. 126, 195–226 (1978)Google Scholar
  8. Furth, M.E., Yates, J.L.: Specificity determinants for bacteriophage lambda DNA replication. II. Structure of O proteins of λ-Φ 80 and λ-82 hybrid phages and of a λ mutant defective in the origin of replication. J. Mol. Biol. 126, 227–240 (1978)Google Scholar
  9. Georgopoulos, C.P.: A new bacterial gene (groPC) which affects λ DNA replication. Mol. Gen. Genet. 151, 35–39 (1977)Google Scholar
  10. Georgopoulos, C.P., Herskowitz, I.: Escherichia coli mutants blocked in lambda DNA synthesis. In: The bacteriophage lambda (A.D. Hershey, ed.), pp. 553–564. New York: Cold Spring Harbor Laboratory 1971Google Scholar
  11. Georgopoulos, C.P., Hohn, B.: Identification of a host protein necessary for bacteriophage morphogenesis (the groE gene product). Proc. Nat. Acad. Sci. USA 75, 131–135 (1978)Google Scholar
  12. Georgopoulos, C.P., Lam, B., Lundquist-Heil, A., Rudolph, C.F., Yochem, J., Feiss, M.: Identification of the E. coli dnaK (groPC756) gene product. Mol. Gen. Genet. 172, 143–149 (1979)Google Scholar
  13. Greene, P.J., Betlach, M.C., Boyer, H.W., Goodman, H.M.: The EcoRI restriction endonuclease, In: Methods in molecular biology (R.B. Wickner, ed.) pp. 87–111. New York: Marcel Decker 1974Google Scholar
  14. Hohn, R., Flick, H., Hohn, B.: Petit lambda. A family of particles. J. Mol. Biol. 98, 107–120 (1975)Google Scholar
  15. Itikawa, H., Ryu, J-I: Isolation and characterization of a temperature-sensitive dnaK mutant of Escherichia coli B. J. Bact. 138, 339–344 (1979)Google Scholar
  16. Jacob, F., Brenner, S., Cuzin, F.: On the regulation of DNA replication in bacteria. Cold Spring Harbor Symp. Quant. Biol. 28, 329–348 (1963)Google Scholar
  17. Kellenberger-Gujer, G., Podhajska, A.J.: Interactions between the plasmid λdv and Escherichia coli dnaA mutants. Mol. Gen. Genet. 162, 17–22 (1978)Google Scholar
  18. Klinkert, J., Klein, A.: Roles of bacteriophage lambda gene products O and P during early and late phases of infection cycle. J. Virol. 25, 730–737 (1978)Google Scholar
  19. Kornberg, A.: DNA Synthesis. San Francisco: W.H. Freeman and Company 1974Google Scholar
  20. Lusky, M., Hobom, G.: Inceptor and origin of DNA replication in lambdoid coliphages I. The λ DNA minimal replication system. Gene 6, 137–172 (1979)Google Scholar
  21. McMacken, R., Kessler, S., Boyce, R.: Strand breakage of coliphage λ DNA supercoils in infected lysogens. I. Genetic and biochemical evidence of two types of nicking processes. Virology 66, 356–371 (1975)Google Scholar
  22. Murray, K., Murray, N.E.: Phage lambda receptor chromosomes for DNA fragments made with restriction endonucleases III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli. J. Biol. 98, 551–564 (1975)Google Scholar
  23. Oppenheim, A., Katzir, N., Oppenheim, A.B.: The product of gene P of coliphage λ. Virology 79, 437–441 (1977)Google Scholar
  24. Rosner, I.L.: Formation, induction and curing of bacteriophage P1 lysogens. Virology 48, 679–689 (1972)Google Scholar
  25. Saito, H., Nakamura, Y., Uchida, H.: A transducing λ phage carrying grpE, a bacterial gene necessary for λ DNA replication, and two ribosomal protein genes, rpsP (S16) and rplS (L19). Mol. Gen. Genet. 165, 247–256 (1978)Google Scholar
  26. Saito, H., Uchida, H.: Initiation of the DNA replication of bacteriophage lambda in Escherichia coli K12. J. Mol. Biol. 113, 1–25 (1977)Google Scholar
  27. Saito, H., Uchida, H.: Organization and expression of the dnaJ and dnaK genes of Escherichia coli K12. Mol. Gen. Genet. 164, 1–8 (1978)Google Scholar
  28. Sunshine, M., Feiss, M., Stuart, J., Yochem, J.: A new host gene (groPC) necessary for lambda DNA replication. Mol. Gen. Genet. 151, 27–34 (1977)Google Scholar
  29. Tomizawa, J.: Functional cooperation of genes O and P. In: The bacteriophage lambda (A.D. Hershey, ed.), pp. 549–552. New York: Cold Spring Harbor Laboratory 1971Google Scholar
  30. Weigle, J.: Assembly of phage lambda in vitro. Proc. Nat. Acad. Sci. USA 55, 1462–1466Google Scholar
  31. Wickner, C.: DNA replication proteins of Escherichia coli and phage λ. Cold Spring Harbor Symp. Quant. Biol. 43, 303–310 (1978a)Google Scholar
  32. Wickner, S.: DNA replication proteins of Escherichia coli. Ann. Rev. Biochem. 47, 1163–1191 (1978b)Google Scholar
  33. Wiman, M., Bertani, G., Kelly, B., Sasaki, I.: Genetic map of Escherichia coli strain C. Mol. Gen. Genet. 107, 1–31 (1970)Google Scholar
  34. Yanofsky, C., Ito, J.: Nonsense codons and polarity in the tryptophan operon. J. Mol. Biol. 21, 313–334 (1966)Google Scholar
  35. Yates, J.L., Gette, W.R., Furth, M.E., Nomura, M.: Effects of ribosomal mutations on the read-through of a chain termination signal: Studies on the synthesis of bacteriophage λ O gene protein in vitro. Proc. Nat. Acad. Sci. USA. 74, 689–693 (1977)Google Scholar
  36. Yochem, J., Uchida, H., Sunshine, M., Saito, H., Georgopoulos, C.P., Feiss, M.: Genetic analysis of two genes dnaJ and dnaK, necessary for Escherichia coli and bacteriophage lambda DNA replication. Mol. Gen. Genet. 164, 9–14 (1978)Google Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • C. P. Georgopoulos
    • 1
  • A. Lundquist-Heil
    • 1
  • J. Yochem
    • 2
  • M. Feiss
    • 2
  1. 1.Department of Cellular, Viral and Molecular Biology, College of MedicineUniversity of UtahSalt Lake CityUSA
  2. 2.Department of Microbiology, College of MedicineThe University of IowaIowa CityUSA

Personalised recommendations