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Molecular and General Genetics MGG

, Volume 189, Issue 2, pp 298–303 | Cite as

Cointegrational transduction and mobilization of gentamicin resistance plasmid pWP14a is mediated by IS140

  • Barbara Bräu
  • Wolfgang Piepersberg
Article

Summary

The structures of two R-plasmids pWP14a and pWP12a (Tra-, Ap, Gm; 21 kb) and of several cointegrates they form with bacteriophages P1Cm and P1–15 were analyzed. In each case, replicon fusion was mediated by the element IS140 (about 0.8 kb), one copy of which resides on both plasmids adjacent to the gentamicin resistance determinant (AAC(3)-III). pWP14a cointegrated preferentially into or near the invertible C-loop structure of the P1 genome. Cointegrational mobilization of pWP14a was observed also with several conjugative R-factors. The process of replicon fusion is independent of the host's rec+ functions.

Sequences homologous to IS140 are constituents of many R-factors, including RA1, R40a, R124, R144, Rts1, N3, and pJR255. IS140 also shows homology to two other sequences, IS15Δ and Tn2680, but not to other, well studied transposable elements. The ampicillin resistance determinant of pWP14a is within a Tn3-like transposon, Tn3651.

Keywords

Ampicillin Gentamicin Transposable Element Resistance Determinant Ampicillin Resistance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Barbara Bräu
    • 1
  • Wolfgang Piepersberg
    • 1
  1. 1.Lehrstuhl für Mikrobiologie der UniversitätMünchen 19Germany

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