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Molecular and General Genetics MGG

, Volume 210, Issue 1, pp 101–110 | Cite as

Identification of components of a new stability system of plasmid R1, ParD, that is close to the origin of replication of this plasmid

  • Alicia Bravo
  • Gertrudis de Torrontegui
  • Ramón Díaz
Article

Summary

We provide evidence that a mutation which derepresses an autoregulated system that is located in the vicinity of the basic replicon of R1, stabilizes the ParA- and ParB- miniplasmid of R1 pKN1562, without increasing its copy number. The system, which we have called ParD, maps inside the 1.45-kb PstI-EcoRI fragment that is adjacent to the origin of replication of the plasmid. Two protiens whose expression is coordinated are components of the system. The sequence of the PstI-EcoRI fragment was obtained. The wild-type ParD system determines in cis a basal but detectable stability.

Key words

Plasmid R1 Stability functions 

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Alicia Bravo
    • 1
  • Gertrudis de Torrontegui
    • 1
  • Ramón Díaz
    • 1
  1. 1.Centro de Investigaciones Biológicas (C.S.I.C.)MadridSpain

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