Identification of components of a new stability system of plasmid R1, ParD, that is close to the origin of replication of this plasmid
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- Bravo, A., de Torrontegui, G. & Díaz, R. Mol Gen Genet (1987) 210: 101. doi:10.1007/BF00337764
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We provide evidence that a mutation which derepresses an autoregulated system that is located in the vicinity of the basic replicon of R1, stabilizes the ParA- and ParB- miniplasmid of R1 pKN1562, without increasing its copy number. The system, which we have called ParD, maps inside the 1.45-kb PstI-EcoRI fragment that is adjacent to the origin of replication of the plasmid. Two protiens whose expression is coordinated are components of the system. The sequence of the PstI-EcoRI fragment was obtained. The wild-type ParD system determines in cis a basal but detectable stability.