Molecular and General Genetics MGG

, Volume 210, Issue 1, pp 69–76

A genetic map of Phycomyces blakesleeanus

  • Margarita Orejas
  • Maria Isabel Peláez
  • Maria Isabel Alvarez
  • Arturo P. Eslava
Article

Summary

Complementation tests among Phycomyces auxotrophic strains revealed the existence of four genes with mutants requiring riboflavin, three genes with purine auxotrophs, two with nicotinic acid auxotrophs, and two with lysine auxotrophs. A total of 134 sexual crosses between strains carrying mutations affecting phototropism (madA-madE), carotenoid biosynthesis (carA), auxotrophy (ribA-ribD, purA-purC, lysA and lysB, nicA and nicB, and leuA) and resistance to 5-fluorouracil (furA and furB) were studied; mating type (sex) was also included as a marker. The results from random spore analysis, tetrad analysis, and gene-centromere distances shows that these markers are distributed into 11 linkage groups.

Key words

Phycomyces Genetic map Tetrad analysis 

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Margarita Orejas
    • 1
  • Maria Isabel Peláez
    • 2
  • Maria Isabel Alvarez
    • 1
  • Arturo P. Eslava
    • 1
  1. 1.Departamento de Genética, Facultad de BiologíaUniversidad de SalamancaSalamancaSpain
  2. 2.Departamento de Genética, Facultad de BiologíaUniversidad de LeónLeónSpain
  3. 3.Department of PhysicsSyracuse UniversitySyracuseUSA

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