Molecular and General Genetics MGG

, Volume 210, Issue 1, pp 69–76 | Cite as

A genetic map of Phycomyces blakesleeanus

  • Margarita Orejas
  • Maria Isabel Peláez
  • Maria Isabel Alvarez
  • Arturo P. Eslava


Complementation tests among Phycomyces auxotrophic strains revealed the existence of four genes with mutants requiring riboflavin, three genes with purine auxotrophs, two with nicotinic acid auxotrophs, and two with lysine auxotrophs. A total of 134 sexual crosses between strains carrying mutations affecting phototropism (madA-madE), carotenoid biosynthesis (carA), auxotrophy (ribA-ribD, purA-purC, lysA and lysB, nicA and nicB, and leuA) and resistance to 5-fluorouracil (furA and furB) were studied; mating type (sex) was also included as a marker. The results from random spore analysis, tetrad analysis, and gene-centromere distances shows that these markers are distributed into 11 linkage groups.

Key words

Phycomyces Genetic map Tetrad analysis 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Alvarez MI, Eslava AP (1983) Isogenic strains of Phycomyces blakesleeanus suitable for genetic analysis. Genetics 105:873–879Google Scholar
  2. Alvarez MI, Peláez MI, Eslava AP (1980) Recombination between ten markers in Phycomyces. Mol Gen Genet 179:447–452Google Scholar
  3. Bergman K, Burke PV, Cerdá-Olmedo E, David CN, Delbrück M, Foster KW, Goodell EW, Heisenberg M, Meissner G, Zalokar M, Dennison DS, Shoropshire W Jr (1969) Phycomyces. Bacteriol Rev 33:99–157Google Scholar
  4. Bergman K, Eslava AP, Cerdá-Olmedo E (1973) Mutants of Phycomyces with abnormal phototropism. Mol Gen Genet 123:1–16Google Scholar
  5. Blakeslee AF (1904) Sexual reproduction in the Mucorineae. Proc Am Acad Arts Sci 40:205–319Google Scholar
  6. Blakeslee AF (1906) Zygospore germinations in the Mucorineae. Ann Mycol 4:1–28Google Scholar
  7. Burgeff H (1915) Untersuchungen über Variabilitat, Sexualität und Erblichkeit bei Phycomyces nitens Kunze II. Flora 108:351–448Google Scholar
  8. Burgeff H (1928) Variabilitat, Vererbung und Mutation bei Phycomyces blakesleeanus Bgff. Z Indukt Abstamm Unters Vererbungsl 49:26–94Google Scholar
  9. Catcheside DG (1974) Fungal Genetics. Annu Rev Genet 8:279–300Google Scholar
  10. Catcheside DG (1975) Occurrence in wild strains of Neurospora crassa of genes controlling genetic recombination. Aust J Biol Sci 28:213–225Google Scholar
  11. Cerdá-Olmedo E (1975) The genetics of Phycomyces blakesleeanus. Genet Res 25:285–296Google Scholar
  12. Cerdá-Olmedo E (1977) Behavioral genetics of Phycomyces. Annu Rev Microbiol 31:535–547Google Scholar
  13. Cerdá-Olmedo E (1984) Genetic determination of the responses of Phycomyces to light. In: Sender H (ed) Blue light effects in biological systems. Springer, Berlin Heidelberg New York Tokyo, pp 220–227Google Scholar
  14. Cerdá-Olmedo E (1985) Carotene mutants of Phycomyces. Methods Enzymol 110:220–243Google Scholar
  15. Eslava AP, Alvarez MI, Burke PV, Delbrück M (1975a) Genetic recombination in sexual crosses of Phycomyces. Genetics 80:445–462Google Scholar
  16. Eslava AP, Alvarez MI, Delbrück M (1975b) Meiosis in Phycomyces. Proc Natl Acad Sci USA 72:4076–4080Google Scholar
  17. Eslava AP, Alvarez MI, Lipson ED, Presti D, Kong K (1976) Recombination between mutants of Phycomyces with abnormal phototropism. Mol Gen Genet 147:235–241Google Scholar
  18. Fincham, JRS, Day PR, Radford A (1979) Fungal Genetics, 4th Edn. Blackwell Scientific Publications. OxfordGoogle Scholar
  19. Galland P, Lipson ED (1984) Photophysiology of Phycomyces blakesleeanus Photochem Photobiol 40:795–800Google Scholar
  20. Garcés R, Chmielewicz CL, Lipson ED (1986) Phycomyces: a new gene for a flavoprotein with covalently linked cofactor. Mol Gen Genet 203:341–345Google Scholar
  21. Gauger W, Peláez MI, Alvarez MI, Eslava AP (1980) Mating type heterokaryons in Phycomyces blakesleeanus. Exp Mycol 4:56–64Google Scholar
  22. Lipson ED (1983) Sensory information processing at the cellular level: the light response systems of Phycomyces. In: Scott GP, Wahner HW (eds) Radiation and cellular response. Iowa State University Press, Ames, Iowa, pp 133–152Google Scholar
  23. Lipson ED, Terasaka DT, Silverstein PS (1980) Double mutants of Phycomyces with abnormal phototropism. Mol Gen Genet 179:155–162Google Scholar
  24. López-Díaz I, Lipson ED (1983) Genetic analysis of hypertropic mutants of Phycomyces. Mol Gen Genet 190:318–325Google Scholar
  25. Pollock JA, Lipson ED, Sullivan DT (1985) Electrophoretic analysis of proteins from night-blind mutants of Phycomyces. Biochem Genet 23:379–390Google Scholar
  26. Revuelta JL, Eslava AP (1983) A new gene (carC) involved in the regulation of carotenogenesis in Phycomyces. Mol Gen Genet 192:225–229Google Scholar
  27. Revuelta JL, Jayaram M (1986) Transformation of Phycomyces blakesleeanus to G-418 resistance by an autonomously replicating plasmid. Proc Natl Acad Sci USA 83:7344–7347Google Scholar
  28. Roncero MIG, Cerdá-Olmedo E (1982) Genetics of carotene biosynthesis in Phycomyces. Curr Genet 5:5–8Google Scholar
  29. Stamberg J (1968) Genetic control of recombination in Schizophylium commune: The occurrence and significance of natural variation. Heredity 24:361–368Google Scholar
  30. Suárez T (1985) Obtención de protoplastos y transformación en Phycomyces blakesleeanus. Resumen de Tesis Doctorales. Ediciones Universidad de Salamanca. Salamanca, p 38Google Scholar
  31. Sutter RP (1975) Mutation affecting, sexual development in Phycomyces blakesleeanus. Proc Natl Acad Sci USA 72:127–130Google Scholar
  32. Sutter RP (1977) Regulation of the first stage of sexual development in Phycomyces blakesleeanus and in other mucoraceous fungi. In: O'Day DH, Horgen PA (eds) Eukaryotic Microbes as model developmental systems. Marcel Decker, New York, pp 251–272Google Scholar
  33. Sutter RP, Whitaker JP (1981) Zygophore-stimulating precursors (pheromones) of trisporic acids active in (-) Phycomyces blakesleeanus. J Biol Chem 256:2334–2341Google Scholar
  34. Torres-Martinez S, Murillo FJ, Cerdá-Olmedo E (1980) Genetics of lycopene cyclization and substrate transfer in β-carotene biosynthesis in Phycomyces. Genet Res 36:299–309Google Scholar
  35. Whitehouse HLK (1949) Multiple allelomorph heterothallism in the fungi. New Phytol 48:212–244Google Scholar
  36. Whitehouse HLK (1950) Mapping chromosome centromeres by the analysis of unordered tetrads. Nature 165:893Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Margarita Orejas
    • 1
  • Maria Isabel Peláez
    • 2
  • Maria Isabel Alvarez
    • 1
  • Arturo P. Eslava
    • 1
  1. 1.Departamento de Genética, Facultad de BiologíaUniversidad de SalamancaSalamancaSpain
  2. 2.Departamento de Genética, Facultad de BiologíaUniversidad de LeónLeónSpain

Personalised recommendations