Molecular and General Genetics MGG

, Volume 214, Issue 2, pp 313–316 | Cite as

Alleviation of type I restriction in adenine methylase (dam) mutants of Escherichia coli

  • E. P. Efimova
  • E. P. Delver
  • A. A. Belogurov


The host-controlled EcoK-restriction of unmodified phage λ.O is alleviated in dam mutants of Escherichia coli by 100- to 300-fold. In addition, the EcoK modification activity is substantially decreased in dam- strains. We show that type I restriction (EcoB, EcoD and EcoK) is detectably alleviated in dam mutants. However, no relief of EcoRI restriction (Type II) occurs in dam- strains and only a slight effect of dam mutation on EcoP1 restriction (Type III) is observed. We interpret the alleviation of the type I restriction in dam- strains to be a consequence of induction of the function which interferes with type I restriction systems.

Key words

DNA restriction Dam mutation Alleviation of restriction Bacteriophage λ 


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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • E. P. Efimova
    • 1
  • E. P. Delver
    • 1
  • A. A. Belogurov
    • 1
  1. 1.Institute of Experimental Cardiology, National Cardiology Research CenterUSSR Academy of Medical SciencesMoscowUSSR

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