Molecular and General Genetics MGG

, Volume 105, Issue 3, pp 219–224 | Cite as

Genetic studies of the ribosomal proteins in Escherichia coli

II. Altered 30s ribosomal protein component specific to spectinomycin resistant mutants
  • Satoshi Dekio
  • Renkichi Takata
Article
Received:

Summary

Spectinomycin resistant (spcr) mutants were obtained by treating the cells of E. coli K12, W3637 with nitrosoguanidine. The compositions of ribosomal proteins were analyzed for six out of eleven such spcr-mutants with chromatography on a carboxymethyl cellulose (CMC) column. The 30s ribosomal subunit from all of the spcr-mutants was found to contain the altered 30-4 protein component, while no difference was detected in 50s ribosomal proteins between spcr and spcs bacteria.

Keywords

Cellulose Chromatography Genetic Study Ribosomal Protein Carboxymethyl 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations used

CMC

carboxymethyl cellulose

str

streptomycin

spc

spectinomycin

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References

  1. Apirion, D., Schlessinger, D.: Corresistance to neomycin and kanamycin by mutations in Escherichia coli locus that affects ribosomes. J. Bact. 96, 768–776 (1968a).Google Scholar
  2. —: Mapping and complementation of three genes specifying 30s ribosomal components in Escherichia coli. J. Bact. 96, 1431–1432 (1968b).Google Scholar
  3. Davies, J., Anderson, P., Davis, B. D.: Inhibition of protein synthesis by spectinomycin. Science 149, 1096–1098 (1965).Google Scholar
  4. Flaks, J. G., Leboy, P. S., Birge, E. A., Kurland, C. G.: Mutations and genetics concerned with the ribosome. Cold Spr. Harb. Symp. quant. Biol. 28, 623–631 (1966).Google Scholar
  5. Leboy, P. S., Cox, E. C., Flaks, J. G.: The chromosomal site specifying a ribosomal protein in Escherichia coli. Proc. nat. Acad. Sci. (Wash.) 52, 1367–1374 (1964).Google Scholar
  6. Mayuga, C., Meier, D., Wang, T.: Escherichia coli: the K12 ribosomal protein and the streptomycin region of the chromosome. Biochem. biophys. Res. Commun. 33, 203–206 (1968).Google Scholar
  7. Otaka, E., Itoh, T., Osawa, S.: Ribosomal proteins of bacterial cells: strain and species-specificity. J. molec. Biol. 33, 93–107 (1968).Google Scholar
  8. Takata, R., Dekio, S., Otaka, E., Osawa, S.: Genetic studies of the ribosomal proteins in Escherichia coli I. Mutants and strains having 30s ribosomal subunit with altered protein components. Molec. Gen. Genetics 105, 113–121 (1969).Google Scholar
  9. Tanaka, Y., Kaji, H.: The role of ribosomal protein for the binding of dihydrostreptomycin to ribosomes. Biochem. biophys. Res. Commun. 32, 313–319 (1968).Google Scholar
  10. Traub, P., Nomura, M.: Streptomycin resistance mutation in Escherichia coli: altered ribosomal protein. Science 160, 198–199 (1968).Google Scholar

Copyright information

© Springer-Verlag 1969

Authors and Affiliations

  • Satoshi Dekio
    • 1
  • Renkichi Takata
    • 1
  1. 1.Department of Biochemistry and Biophysics, Research Institute for Nuclear Medicine and BiologyHiroshima UniversityHiroshimaJapan

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