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Current Genetics

, Volume 23, Issue 1, pp 19–21 | Cite as

Evolutionary conservation of genomic sequences related to the GGP1 gene encoding a yeast GPI-anchored glycoprotein

  • Marina Vai
  • Emanuela Lacanà
  • Evelina Gatti
  • Diego Breviario
  • Laura Popolo
  • Lilia Alberghina
Original Articles

Summary

The GGP1 gene encodes the only GPI-anchored glycoprotein (gp115) that has been purified todate in the budding yeast Saccharomyces cerevisiae. It is a single-copy gene whose deduced amino-acid sequence shares no significant homology to any other known protein. In this paper we report a Southern hybridization analysis of genomic DNA from different eukaryotic organisms to identify homologues of the GGP1 gene. We have analyzed DNA prepared from a unicellular green alga (Chlamydomonas eugametos), from two distantly related yeast species (Candida cylindracea and Schizosaccharomyces pombe), and from the common bean Phasoleus vulgaris. The moderate stringency of the experimental conditions and the high specificity of the probes used indicate that a single-copy of GGP1-related sequences exists in all these eukaryotic organisms. The chromosomal localization of the GGP1 gene in S. cerevisiae has also been determined.

Key words

Glycosylphosphatidylinositol anchored-protein Southern analysis Saccharomyces cerevisiae 

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Marina Vai
    • 1
  • Emanuela Lacanà
    • 1
  • Evelina Gatti
    • 1
  • Diego Breviario
    • 2
  • Laura Popolo
    • 1
  • Lilia Alberghina
    • 1
  1. 1.Sezione di Biochimica Comparata, Dipartimento di Fisiologia e Biochimica GeneraliUniversità di MilanoMilanoItaly
  2. 2.Istituto di Biosintesi VegetaliCNRMilanoItaly

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