Current Genetics

, Volume 19, Issue 3, pp 199–206 | Cite as

Differential expression of the psbB and psbH genes encoding the 47 kDa chlorophyll a-protein and the 10 kDa phosphoprotein of photosystem II during chloroplast development in wheat

  • Sean M. Hird
  • Andrew N. Webber
  • Rebecca J. Wilson
  • Tristan A. Dyer
  • John C. Gray
Original Articles
Received:
Accepted:

Summary

The nucleotide sequence of a region of wheat chloroplast DNA containing the psbB gene for the 47 kDa chlorophyll a-binding protein of photosystem II has been determined. The gene encodes a polypeptide of 508 amino acid residues which is predicted to contain six hydrophobic membrane-spanning regions. The psbB gene is located 562 bp upstream of the psbH gene for the 10 kDa phosphoprotein of photosystem II. A small open reading frame of 38 codons is located between psbB and psbH, and on the opposite strand the psbN gene, encoding a photosystem II polypeptide of 43 amino acid residues, is located between orf38 and psbH. S1 nuclease mapping indicated that the 5′ ends of transcripts were located 371 and 183 bp upstream of the psbB translation initiation codon. Predominant transcripts of 2.1 kb and 1.8 kb for psbB and 0.4 kb for psbH were present in RNA isolated from etiolated and greening wheat seedlings. Immunodecoration of Western blots indicated that the 47 kDa polypeptide was absent, or present in very low amounts, in dark-grown tissue and accumulated on greening, whereas the 10 kDa polypeptide was present in similar amounts in both dark-grown and greening seedlings. The 10 kDa polypeptide was phosphorylated in vitro by incubating wheat etioplast membranes with [γ3 2P] ATP.

Key words

Light regulation psbN Triticum aestivum Etioplast 
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References

  1. Allen JF, Holmes NG (1986) FEBS Lett 202:175–181Google Scholar
  2. Alt J, Morris J, Westhoff P, Herrmann RG (1984) Curr Genet 8:597–606Google Scholar
  3. Apel K (1979) Eur J Biochem 97:183–188Google Scholar
  4. Apel K, Kloppstech K (1980) Planta 150:426–430Google Scholar
  5. Baker NR, Markwell JP, Webber AN, Thornber JP (1984) Adv Photosynth Res 3:319–322Google Scholar
  6. Barkan A (1988) EMBO J 7:2637–2644Google Scholar
  7. Barker RF, Idler DB, Thompson DV, Kemp JD (1983) Plant Mol Biol 2:335–350Google Scholar
  8. Bartlett S, Grossman AR, Chua N-H (1982) In Edelman M, Hallick R, Chua N-H (eds) Methods in chloroplast molecular biology Elsevier, Amsterdam, pp 1081–1091Google Scholar
  9. Batschauer A, Mösinger E, Kreuz K, Dörr I, Apel K (1986) Eur J Biochem 154:625–634Google Scholar
  10. Bennett J (1981) Eur J Biochem 118:61–70Google Scholar
  11. Berk AJ, Sharp PA (1977) Cell 12:721–732Google Scholar
  12. Berthold DA, Babcock GT, Yocum CJ (1981) FEBS Lett 134:231–234Google Scholar
  13. Biggin MD, Gibson TJ, Hong GF (1983) Proc Natl Acad Sci USA 80:3963–3965Google Scholar
  14. Bricker TM, Odom WR, Queirolo CB (1988) FEBS Lett 231:111–117Google Scholar
  15. Burgess DG, Taylor WC (1987) Planta 170:520–527Google Scholar
  16. Camm EL, Green BR (1983) J Cell Biochem 23:171–179Google Scholar
  17. Courtice GRM, Bowman CM, Dyer TA, Gray JC (1985) Curr Genet 10:329–333Google Scholar
  18. Covello P, Webber AN, Danko SJ, Markwell JP, Baker NR (1987) Photosynth Res 12:243–254Google Scholar
  19. Covey SN, Hull R (1981) Virology 111:463–474Google Scholar
  20. Cuming AC, Bennett J (1981) Eur J Biochem 118:71–80Google Scholar
  21. Enami I, Satoh K, Katoh S (1987) FEBS Lett 226:161–165Google Scholar
  22. Feinberg AP, Vogelstein B (1983) Anal Biochem 132:6–13Google Scholar
  23. Fling SP, Gregerson DS (1986) Anal Biochem 155:83–88Google Scholar
  24. Fromm H, Devic M, Fluhr R, Edelman M (1985) EMBO J 4:291–295Google Scholar
  25. Hallick RB (1989) Plant Mol Biol Rep 7:266–275Google Scholar
  26. Hinz U (1985) Carlsberg Res Commun 50:285–298Google Scholar
  27. Hiratsuka J, Shimada H, Whittier R, Ishibashi T, Sakamoto M, Mori M, Kondo C, Honji Y, Sun CR, Meng BY, Li YQ, Kanno A, Nishizawa Y, Hirai A, Shinozaki K, Sugiura M (1989) Mol Gen Genet 217:185–194Google Scholar
  28. Hird SM, Dyer TA, Gray JC (1986) FEBS Lett 209:181–186Google Scholar
  29. Holschuh K, Bottomley W, Whitfeld PR (1984) Nucleic Acids Res 12:8819–8834Google Scholar
  30. Ikeuchi M, Inoue Y (1988) FEBS Lett 241:99–104Google Scholar
  31. Ikeuchi M, Koike H, Inoue Y (1989) FEBS Lett 253:178–182Google Scholar
  32. Klein RR, Mullet JE (1987) J Biol Chem 262:4341–4348Google Scholar
  33. Klein RR, Gamble PE, Mullet JE (1988) Plant Physiol 88:1246–1256Google Scholar
  34. Kohchi T, Yoshida T, Komano T, Ohyama K (1988) EMBO J 7:885–891Google Scholar
  35. Laemmli UK (1970) Nature 227:680–685Google Scholar
  36. Lamppa GK, Morelli G, Chua N-H (1985) Mol Cell Biol 5:1370–1378Google Scholar
  37. Matsudaira P (1987) J Biol Chem 262:10035–10038Google Scholar
  38. Mayfield SP, Taylor WC (1984) Eur J Biochem 144:79–84Google Scholar
  39. Mayfield SP, Taylor WC (1987) Mol Gen Genet 208:309–314Google Scholar
  40. Maxam A, Gilbert W (1980) Methods Enzymol 65:499–560Google Scholar
  41. Michel HP, Hunt DF, Shabanowitz J, Bennett J (1988) J Biol Chem 263:1123–1130Google Scholar
  42. Morris J, Herrmann RG (1984) Nucleic Acids Res 12:2837–2850Google Scholar
  43. Murata N, Miyao M, Hayashida N, Hidaka T, Sugiura M (1988) FEBS Lett 235:283–288Google Scholar
  44. Nakatani H HY, Ke B, Dolan E, Arntzen CJ (1984) Biochim Biophys Acta 765:347–352Google Scholar
  45. Nanba O, Satoh K (1987) Proc Natl Acad Sci USA 84:109–112Google Scholar
  46. Newman BJ, Gray JC (1988) Plant Mol Biol 10:511–520Google Scholar
  47. Ohyama K, Fukuzawa H, Kohchi T, Shirai H, Sano T, Sano S, Umesono K, Shiki Y, Takeuchi M, Chang Z, Aota S, Inokuchi H, Ozeki H (1986) Nature 322:572–574Google Scholar
  48. Packham NK (1988) FEBS Lett 231:284–290Google Scholar
  49. Rock CD, Barkan A, Taylor WC (1987) Curr Genet 12:69–77Google Scholar
  50. Sanger F, Coulson AR, Barrell BG, Smith AJH, Roe BA (1980) J Mol Biol 143:161–178Google Scholar
  51. Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986) EMBO J 5:2043–2049Google Scholar
  52. Stanley KK, Luzio JP (1984) EMBO J 3:1429–1434Google Scholar
  53. Tanaka M, Obokata J, Chunwongse J, Shinozaki K, Sugiura M (1987) Mol Gen Genet 209:427–431Google Scholar
  54. Towbin H, Staehelin T, Gordon J (1979) Proc Natl Acad Sci USA 76, 4350–4354Google Scholar
  55. Weaver RF, Weissmann C (1979) Nucleic Acids Res 7:1175–1193Google Scholar
  56. Webber AN, Hird SM, Packman L, Dyer TA, Gray JC (1989a) Plant Mol Biol 12:141–151Google Scholar
  57. Webber AN, Packman L, Chapman DJ, Barber J, Gray JC (1989b) FEBS Lett 242:259–262Google Scholar
  58. Westhoff P (1985) Mol Gen Genet 201:115–123Google Scholar
  59. Westhoff P, Herrmann RG (1988) Eur J Biochem 171:551–564Google Scholar
  60. Westhoff P, Farchaus JW, Herrmann RG (1986) Curr Genet 11:165–169Google Scholar
  61. Yen TSB, Webster RE (1981) J Biol Chem 256:11259–11265Google Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Sean M. Hird
    • 1
    • 2
  • Andrew N. Webber
    • 1
  • Rebecca J. Wilson
    • 1
  • Tristan A. Dyer
    • 2
  • John C. Gray
    • 1
  1. 1.Botany SchoolUniversity of CambridgeCambridgeUK
  2. 2.Institute of Plant Science Research(Cambridge Laboratory)CambridgeUK

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