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Current Genetics

, Volume 19, Issue 3, pp 191–197 | Cite as

Splicing of the Petunia cytochrome oxidase subunit II intron

  • Kim D. Pruitt
  • Maureen R. Hanson
Original Articles

Summary

A comparative analysis of the plant introncontaining mitochondrial cytochrome oxidase subunit II (coxII) genes provides an indication that four conserved sequence motifs, present in exon 1 (intron-binding sequences; IBS), and complementary motifs (exon-binding sequences; EBS), present in domain I of the group II intron, may be involved in splicing of the intron. Two of these potential IBS motifs (IBS1 and IBS2) have been previously discussed. Two further potential IBS motifs (IBSa and IBSb), which occur twice within exon 1, could be involved in specification of the 5′ splice site and of a 5′ cryptic splice site. Nuclease-proection experiments and DNA sequence analysis of a spliced coxII cDNA have confirmed the predicted positions of the petunia coxII 5′ and 3′ splice sites. Evidence for the occurrence of splicing in vivo at the putative 5′ cryptic splice site in petunia is provided by the detection of a nuclease-protected fragment corresponding to the size which is predicted if splicing at the proposed cryptic splice site occurs. The existence and location of a cryptic splice site, upstream of the normal coxII 5′ splice site, is consistent with the proposed derivation of the cytoplasmic male sterility (CMS)-associated pcf gene from an abnormally spliced coxII transcript (Pruitt and Hanson 1989).

Key words

Group II intron Cryptic splice site Cytochrome oxidase subunit II Plant mitochondria 

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References

  1. Ahne A, Muller-Derlich J, Merlos-Lange AM, Kanbay F, Wolf K, Lang BF (1988) J Mol Biol 202:725–734Google Scholar
  2. Bonen L, Boer PH, Gray MW (1984) EMBO J 3:2531–2536Google Scholar
  3. Covello PS and Gray MW (1989) Nature 341:662–666Google Scholar
  4. Gualberto JM, Lamattina L, Bonnard G, Weil JH, Grienenberger JM (1989) Nature 341:660–663Google Scholar
  5. Fox TD, Leaver CJ (1981) Cell 26:315–323Google Scholar
  6. Hanson MR, Boeshore ML, McClean PE, O'connell MA, Nivison HT (1986) Methods Enzymol 118:437–453Google Scholar
  7. Heisel R, Wissinger B, Schuster W, Brennicke A (1989) Science 246:1632–1634Google Scholar
  8. Jacquier A, Michel F (1987) Cell 50:17–29Google Scholar
  9. Jarrell KA, Peebles CL, Dietrich RC, Romiti SL, Perlman PS (1988) J Biol Chem 263:3432–3439Google Scholar
  10. Kao TH, Moon E, Wu R (1984) Nucleic Acids Res 12:7305–7315Google Scholar
  11. Michel F, Umesono K, Ozeki H (1989) Gene 82:5–30Google Scholar
  12. Muller MW, Schweyen RJ, Schmelzer C (1988) Nucleic Acids Research 16:7383–7395Google Scholar
  13. Pruitt KD, Hanson MR (1989) Curr Genet 16:281–291Google Scholar
  14. Young EG, Hanson MR (1987) Cell 50:41–49Google Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Kim D. Pruitt
    • 1
  • Maureen R. Hanson
    • 1
  1. 1.Section of Genetics and DevelopmentCornell UniversityIthacaUSA

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