Biology and Fertility of Soils

, Volume 14, Issue 2, pp 126–134 | Cite as

Effects of soil texture and structure on carbon and nitrogen mineralization in grassland soils

  • J. Hassink


The hypotheses that disruption of soil structure increases mineralization rates in loams and clays more than in sandy soils and that this increase can be used to estimate the fraction of physically protected organic matter were tested. C and N mineralization was measured in undisturbed, and in finely and coarsely sieved moist or dried/remoistened soil. Fine sieving caused a temporary increase in mineralization. The relative increase in mineralization was much larger in loams and clays than in sandy soils and much larger for N than for C. The combination of remoistening and sieving of the soil gave a further increase in the mineralization flush after the disturbance. Again, the extra flush was larger in loams and clays than in sandy soils, and larger for N than for C. In loams and clays, small pores constituted a higher percentage of the total pore space than in sandy soils. The fraction of small pores explained more than 50% of the variation in the N mineralization rate between soils. There was also a good correlation between the small-pore fraction and the relative increase in N mineralization with fine sieving. For C, these relations were not clear. It is suggested that a large part of the organic matter that was present in the small pores could not be reached by microorganisms, and was therefore physically protected against decomposition. Fine sieving exposed part of this fraction to decomposition. This physically protected organic matter had a lower C: N ratio than the rest of the soil organic matter. The increase in N mineralization after fine sieving can be regarded as a measure of physically protected organic matter.

Key words

Grassland Mineralization Soil texture Soil structure Physical protection 


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  1. Bottner P (1985) Response of microbial biomass to alternate moist and dry conditions in a soil incubated with 14C- and 15N-labelled plant material. Soil Biol Biochem 17: 329–337Google Scholar
  2. Cabrera ML, Kissel DE (1988) Potentially mineralizable nitrogen in disturbed and undisturbed soil samples. Soil Sci Soc Am J 52: 1010–1015Google Scholar
  3. Cameron RS, Posner AM (1979) Mineralizable organic nitrogen in soil fractionated according to particle size. J Soil Sci 30: 565–577Google Scholar
  4. Catroux G, Chaussod R, Nicolardot B (1987) Assessment of nitrogen supply from the soil. R. R. Acad Agric Francais 3: 71–79Google Scholar
  5. Chichester FW (1969) Nitrogen in soil organo-mineral sedimentation fractions. Soil Sci 107: 356–363Google Scholar
  6. Deijs WB (1961) The determination of total nitrogen in herbage samples (in Dutch). Inst Biol Scheikond Jaarb Meded 89, 158:90Google Scholar
  7. Elliott ET, Coleman DC (1988) Let the soil work for us. In: Eijsackers H, Quispel A (eds) Ecological implications of contemporary agriculture. Proc 4th European Ecology Symposium, Wageningen. Ecol Bull (Copenhagen) 39: 23–32Google Scholar
  8. Elliot ET, Anderson RV, Coleman DC, Cole CV (1980) Habitable pore space and microbial trophic interactions. Oikos 35: 327–335Google Scholar
  9. Foster RC (1986) In situ identification of organic materials in soils. Questiones Entomol 21: 609–633Google Scholar
  10. Foster RC (1988) Microenvironments of soil microorganisms. Biol Fertil Soils 6: 189–203Google Scholar
  11. Genstat Manual (1987) Genstat, a general statistical program. Clarendon Press, OxfordGoogle Scholar
  12. Gregorich EG, Kachanoski RG, Voroney RP (1989) Carbon mineralization in soil size fractions after various amounts of aggregate disruption. J Soil Sci 40: 649–659Google Scholar
  13. Hassink J, Scholefield D, Blantern P (1990) Nitrogen mineralization in grassland soils. In: Gaborcik N, Krajcovic V, Zimkova M (eds) Proc 13th General Meeting of the European Grassland Federation, vol II. Banscka Bystrica, Tsjechoslovakia, pp 25–32Google Scholar
  14. Jenkinson DS, Powlson DS (1976) The effects of biocidal treatments on metabolism in soil: V. A method for measuring soil biomass. Soil Biol Biochem 8: 209–213Google Scholar
  15. Jocteur-Monrozier L, Ladd JN, Fitzpatrick RW, Foster RC, Raupach M (1991) Components and microbial biomass content of size fractions in soils of contrasting aggregation. Geoderma 49: 37–62Google Scholar
  16. Klute A (1986) Water retention: laboratory methods. In: Klute A (ed) Methods of soil analysis, part I. Physical and mineralogical methods. Agron Monogr 9, Am Soc Agron, Madison, Wisconsin, pp 635–662Google Scholar
  17. Kuikman PJ, Van Veen JA (1989) The impact of protozoa on the availability of bacterial nitrogen to plants. Biol Fertil Soils 8: 13–18Google Scholar
  18. Ladd JN, Amato M, Jocteur-Monrozier L, Van Gestel M (1990) Soil microhabitats and carbon and nitrogen metabolism. In: Proc 14th Int Congr Soil Sci, August 12–18, 1990, Kyoto, Japan, vol III, pp 82–87Google Scholar
  19. Mebius LJ (1960) A rapid method for the determination of organic carbon in soil. Anal Chim Acta 22: 120–124Google Scholar
  20. Nordmeyer H, Richter J (1985) Incubation experiments on nitrogen mineralization in loess and sandy soils. Plant and Soil 83: 433–445Google Scholar
  21. Postma J, Van Veen JA (1990) Habitable pore space and population dynamics of Rhizobium leguminosarum biovar trifolii introduced into soil. Microb Ecol 19: 149–161Google Scholar
  22. Richter J, Nuske A, Habenicht W, Bauer J (1982) Optimized N-mineralization parameters of loess soils from incubation experiments. Plant and Soil 68: 379–388Google Scholar
  23. Schnürer J, Clarholm M, Rosswall T (1985) Microbial biomass and activity in an agricultural soil with different organic matter contents. Soil Biol Biochem 17: 611–618Google Scholar
  24. Schröder D, Gehlen P, Peters H (1989) Bodenmikrobielle Aktivität in gesiebten, aggregierten und ungestörten Proben. Kongreßband 101, VDLUFA-Kongreß Bayreuth, VDLUFA-Schriftreihe 30/1990, pp 461–466Google Scholar
  25. Tisdall JM, Oades JM (1982) Organic matter and water-stable aggregates in soils. J Soil Sci 33: 141–163Google Scholar
  26. Van der Linden AMA, Jeurissen LJJ, Van Veen JA, Schippers B (1989) Turnover of soil microbial biomass as influenced by soil compaction. In: Hansen JA, Henriksen K (eds) Nitrogen in organic wastes applied to soils, Academic Press, London, pp 25–36Google Scholar
  27. Van Veen JA, Kuikman PJ (1990) Soil structural aspects of decomposition of organic matter. Biogeochemistry 11: 213–233Google Scholar
  28. Van Veen JA, Paul EA (1981) Organic carbon dynamics in grassland soils: I. Background information and computer simulation. Can J Soil Sci 61: 185–201Google Scholar
  29. Vargas R, Hattori T (1986) Protozoan predation of bacterial cells in soil aggregates. FEMS Microbiol Ecol 38: 233–242Google Scholar
  30. Verberne ELJ, Hassink J, De Willigen P, Groot JJR, Van Veen JA (1990) Modelling organic matter dynamics in different soils. Neth J Agric Sci 38: 221–238Google Scholar
  31. Woods LE, Cole CV, Elliott ET, Anderson RV, Coleman DC (1982) Nitrogen transformation in soil as affected by bacterial-microfaunal interactions. Soil Biol Biochem 14: 93–98Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • J. Hassink
    • 1
  1. 1.DLO-Institute for Soil Fertility ResearchHarenThe Netherlands

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