Biology and Fertility of Soils

, Volume 14, Issue 2, pp 112–115 | Cite as

Enzymatic hydrolysis of ester sulphate in soil organic matter extracts

  • G. Lou
  • P. R. Warman


A method of assessing the enzymatic hydrolysis of ester sulphate in soil organic matter was developed. Soil organic matter extracted using a mild, chelating resin extraction procedure was incubated with a sulphatase from Helix pomatia in 0.05 M sodium acetate buffer (pH 4–8) at 37°C for 2h and the sulphate released was determined by a high performance liquid chromatography-conductivity detector system. The effect of some soil factors on the enzymatic hydrolysis of ester sulphate was examined. The study showed that part of the ester sulphate in soil organic matter was biochemically reactive. In the three Podzols studied, the ester sulphate hydrolysed accounted for 2%–12% of the hydriodic acid-reducible organic sulphate extracted. The largest amount of hydrolysable ester sulphate was found in the soil with a low pH, high inorganic sulphate and high hydriodic acid-reducible organic sulphate.

Key words

Enzyme Organic sulphur Podzol Soil organic matter Sulphatase Sulphate Sulphur mineralization 


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  1. Adams TM, Lane PW (1984) A comparison of four methods of analysing aqueous soil extracts for sulphate. J Sci Food Agric 35:740–744Google Scholar
  2. Appiah MR, Ahenkorah Y (1989) Determination of available sulphate in some soils of Ghana using five extraction methods. Biol Fertil Soils 8:80–86Google Scholar
  3. Blair G, Lefroy RDB (1991) A new S soil test for crops and pastures. In: Abstracts of International Symposium on Soil Testing and Plant Analysis in the Global Community, Aug 22–27, 1991, Orlando, Florida. Council on Soil Testing and Plant Analysis, Athens, Georgia, USA, p 20Google Scholar
  4. Cooper PJM (1972) Arylsulfatase activity in Northern Nigerian soils. Soil Biol Biochem 4:333–337Google Scholar
  5. David MB, Schindler SC, Mitchell MJ, Strick JE (1983) Importance of organic and inorganic sulfur to mineralization processes in a forest soil. Soil Biol Biochem 15:671–677Google Scholar
  6. Dodgson KS, Rose FA (1975) Sulfohydrolases. In: Greenberg DM (ed) Metabolic pathways, 3rd edn. Vol VII. Metabolism of sulfur compounds. Academic Press. New York, pp 359–431Google Scholar
  7. Fitzgerald JW (1976) Sulfate ester formation and hydrolysis: A potentially important yet often ignored aspect of the sulfur cycle of aerobic soils. Bacteriol Rev 40:698–721Google Scholar
  8. Fitzgerald JW, Ash JT, Strickland TC, Swank WT (1983) Formation of organic sulfur in forest soils: A biologically mediated process. Can J For Res 13:1077–1082Google Scholar
  9. Fitzgerald JW, Watwood ME, Rose FA (1985) Forest floor and soil arylsulphatase: Hydrolysis of tyrosine sulphate, an environmentally relevent substrate for the enzyme. Soil Biol Biochem 17:885–887Google Scholar
  10. Freney JR (1986) Forms and reactions of organic sulfur compounds in soils. In: Tabatabai MA (ed) Sulfur in agriculture. Am Soc Agron, Madison, Wisconsin, pp 207–232Google Scholar
  11. Freney JR, Melville GE, Williams CH (1969) Extraction, chemical nature, and properties of soil organic sulfur. J Sci Food Agric 20:440–445Google Scholar
  12. Hoque S, Heath SB, Killham K (1987) Evaluation of methods to assess adequacy of potential soil S supply to crops. Plant and Soil 101:3–8Google Scholar
  13. Houghton C, Rose FA (1976) Liberation of sulfate from sulfate esters by soils. Appl Environ Microbiol 31:969–976Google Scholar
  14. Kowalenko CG, Lowe WE (1975) Mineralization of sulfur from four soils and its relationship with carbon, nitrogen and phosphorus. Can J Soil Sci 55:9–14Google Scholar
  15. Nelson DW, Sommers LE (1982) Total carbon, organic carbon, and organic matter. In: Page AL, Miller RH, Keeney DR (eds) Methods of soil analysis, 2nd edn Part II. Chemical and microbiological properties. Am Soc Agron, Madison, Wisconsin, pp 539–579Google Scholar
  16. Sarathchandra SU, Perrott KW (1981) Determination of phosphatase and arylsulfatase activities in soils. Soil Biol Biochem 13:543–545Google Scholar
  17. Speir TW, Ross DJ (1978) Soil phosphatase and sulphatase. In: Burns RG (ed) Soil enzymes. Academic Press, London, pp 197–250Google Scholar
  18. Tabatabai MA (1982) Sulfur. In: Page AL, Miller RH, Keeney DR (eds) Methods of soil analysis, 2nd edn. Part II. Chemical and microbiological properties. Am Soc Agron, Madison, Wisconsin, pp 501–538Google Scholar
  19. Tabatabai MA, Bremner JM (1970) Arylsulfatase activity of soils. Soil Sci Soc Am Proc 34:225–229Google Scholar
  20. Tabatabai MA, Bremner JM (1972) Distribution of total and available sulfur in selected soils and soil profiles. Agron J 64:40–44Google Scholar
  21. Warman PR, Bishop C (1985) The use of reverse-phase HPLC for soil amino-N analysis. J Liquid Chromatogr 8:2595–2606Google Scholar
  22. Warman PR, Sampson HG (1992) Evaluation of soil sulfate extractants and methods of analysis for plant available sulfur. Commun Soil Sci Plant Anal 23:793–803Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • G. Lou
    • 1
  • P. R. Warman
    • 1
  1. 1.Chemistry and Soil Science DepartmentNova Scotia Agricultural CollegeTruroCanada

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