Biology and Fertility of Soils

, Volume 14, Issue 2, pp 112–115

Enzymatic hydrolysis of ester sulphate in soil organic matter extracts

  • G. Lou
  • P. R. Warman

DOI: 10.1007/BF00336259

Cite this article as:
Lou, G. & Warman, P.R. Biol Fertil Soils (1992) 14: 112. doi:10.1007/BF00336259


A method of assessing the enzymatic hydrolysis of ester sulphate in soil organic matter was developed. Soil organic matter extracted using a mild, chelating resin extraction procedure was incubated with a sulphatase from Helix pomatia in 0.05 M sodium acetate buffer (pH 4–8) at 37°C for 2h and the sulphate released was determined by a high performance liquid chromatography-conductivity detector system. The effect of some soil factors on the enzymatic hydrolysis of ester sulphate was examined. The study showed that part of the ester sulphate in soil organic matter was biochemically reactive. In the three Podzols studied, the ester sulphate hydrolysed accounted for 2%–12% of the hydriodic acid-reducible organic sulphate extracted. The largest amount of hydrolysable ester sulphate was found in the soil with a low pH, high inorganic sulphate and high hydriodic acid-reducible organic sulphate.

Key words

Enzyme Organic sulphur Podzol Soil organic matter Sulphatase Sulphate Sulphur mineralization 

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • G. Lou
    • 1
  • P. R. Warman
    • 1
  1. 1.Chemistry and Soil Science DepartmentNova Scotia Agricultural CollegeTruroCanada

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