Biology and Fertility of Soils

, Volume 9, Issue 3, pp 268–272 | Cite as

Chitinolytic and proteolytic activity of streptomycetes isolated from root-free soil, rhizosphere and mycorrhizosphere of pine (Pinus sylvestris L.)

  • E. Strzelczyk
  • K. Gorlach
  • H. Rózycki
Article

Summary

We tested 75 strains of Streptomyces spp. (25 taken from each environment of soil, rhizosphere, and mycorrhizosphere of pine, Pinus sylvestris L.) and all exhibited chitinolytic activity and hydrolysed gelatine and sodium caseinate in agar media. Enrichment of these media with glucose and NH4NO3 caused induction or stimulation of proteolytic Streptomyces spp. strains (80%) derived from root-free soil; inhibition of this activity was observed in most strains (92%) isolated from the root zone. The post-culture liquids of the rhizosphere strains cultured in the absence of glucose revealed a significantly higher proteolytic activity than those obtained from the root-free soil. The addition of glucose to the medium stimulated proteolytic activity in the post-culture broth of Streptomyces strains derived from soil and the mycorrhizosphere.

Key words

Streptomycetes Chitinolytic Proteolytic activity Soil Pine roots Rhizosphere Pinus sylvestris 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Buchanan RE, Gibbons NE (1974) Bergey's Manual of Determinative Bacteriology. Williams & Wilkins, BaltimoreGoogle Scholar
  2. Burns RG (1983) Extracellular enzyme-substrate interactions in soil. In: Slater JH, Whittenbury R, Wimpenny JWT (eds) Microbes in their natural environments. Cambridge University Press, Cambridge, pp 249–298Google Scholar
  3. Curl EA, Truelove B (1986) The rhizosphere. Advanced Series in Agriculture Sciences, Springer, Berlin Heidelberg New York TokyoGoogle Scholar
  4. Hellmich S, Schauz K (1988) Production of extracellular alkaline and neutral proteases of Ustilago maydis. Exp Mycol 12:223–232Google Scholar
  5. Lingappa Y, Lockwood JL (1962) Chitin media for selective isolation of actinomycetes. Phytopathology 52:317–323Google Scholar
  6. Lochhead AG, Chase FE (1943) Qualitative studies of soil microorganisms: V. Nutritional requirements of the predominant bacterial flora. Soil Sci 55:185–195Google Scholar
  7. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275Google Scholar
  8. McCaroll DR, Thor E (1985) Pectolytic, cellulolytic and proteolytic activities expressed by cultures of Endothia parasitica and inhibition of these activities by compounds extracted from Chinese and American chestnut inner bark. Physiol Plant Pathol 26:367–378Google Scholar
  9. Mitchell R, Alexander M (1962) Lysis of soil fungi by bacteria. Can J Microbiol 9:169–177Google Scholar
  10. Ordentlich A, Elad Y, Chet I (1988) The role of chitinase of Serratia macrescens in biocontrol of Sclerotium rolfsii. Phytopathology 78:84–88Google Scholar
  11. Reissig JR, Strominger JL, Leloir LF (1955) A modified colorimetric method for the estimation of N-acetylamino sugars. J Biol Chem 217:959–966Google Scholar
  12. Renko M, Pokorny M, Vitale L, Turk V (1981) Streptomyces rimosus extracellular proteases. Eur J Appl Microbiol Biotechnol 11:166–171Google Scholar
  13. Skujinš JJ, Pukite A, McLaren AD (1970) Chitinase of Streptomyces sp.: Purification and properties. Enzymologia 39:353–370Google Scholar
  14. Strzelczyk E, Szpotański T (1989) Cellulolytic and pectolytic activity of streptomycetes isolated from root-free soil, rhizosphere and mycorrhizosphere of pine (Pinus sylvestris L.). Biol Fertil Soils 7:365–369Google Scholar
  15. Szabó IM (1974) Microbial communities in a forest-rendzina ecosystem: The pattern of microbial communities. Akademiai Kiado, BudapestGoogle Scholar
  16. Williams ST, Robinson CS (1981) The role of streptomycetes in decomposition of chitin in acidic soils. J Gen Microbiol 127:55–63Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • E. Strzelczyk
    • 1
  • K. Gorlach
    • 2
  • H. Rózycki
    • 1
  1. 1.Laboratory of Microbiology, Institute of BiologyNicolaus Copernicus UniversityToruńPoland
  2. 2.Laboratory of Agricultural Microbiology, Faculty of AgricultureAgricultural and Technical UniversityBydgoszczPoland

Personalised recommendations