Biology and Fertility of Soils

, Volume 21, Issue 3, pp 197–200

Occurrence of the endophytic diazotrophs Herbaspirillum spp. in roots, stems, and leaves, predominantly of Gramineae

  • Fabio L. Olivares
  • Vera L. D. Baldani
  • Veronica M. Reis
  • Jose I. Baldani
  • Johanna Döbereiner
Original Paper

Abstract

Since the first description of Herbaspirillum seropedicae in 1986, few data have been published on this diazotroph, possibly due to difficulties in isolating it from soil. In the present study we found that this bacterium seems to be an obligate endophyte which has been isolated from roots, stems, and leaves of a large number of samples of more than 10 different species of the Gramineae family, but only exceptionally from other plants. H. rubrisubalbicans, previously misnamed as “Pseudomonas” rubrisubalbicans, and known as a mild pathogen of sugarcane causing mottled stripe disease, confirms the endophytie habitat of this genus. This species occurs in roots, stems, and leaves of sugarcane and seems to be restricted to this crop. Inoculation of strains from both species into soil in high numbers resulted in a rapid decline in their numbers. In only 30 days the population of Herbaspirillum spp. in soil decreased below detection limits (<100 cells g-1). When sorghum was planted in this soil, the bacteria reappeared and multiplied within the plant tissues.

Key words

Herbaspirillum Endophytes of Gramineae Diazotrophs Survival in soil Nitrogen fixation sugarcane 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Baldani JI, Baldani VLD, Seldin L, Döbereiner J (1986) Characterization of Herbaspirillum seropedicae gen. nov., sp. nov. a root-associated nitrogen-fixing bacterium. Int J Syst Bacteriol 36:86–93Google Scholar
  2. Baldani VLD, Baldani JI, Olivares F, Döbereiner J (1992a) Identification and ecology of Herbaspirillum seropedicae an the closely related Pseudomonas rubrisubalbicans. Symbiosis 13:65–73Google Scholar
  3. Baldani VLD, James EK, Baldani JI, Döbereiner J (1992b) Localization of the N2-fixing bacterium Herbaspirillum seropedicae within root cells of rice. An Acad Bras Ciên 64:431Google Scholar
  4. Döbereiner J (1992) The genera Azospirillum and Herbaspirillum. In: Balows A, Trüper HG, Dworkin M, Harder W, Schleifer KH (eds) The prokaryotes 2nd edn, vol III. Springer Verlag, New York Berlin Heidelberg London Paris Tokyo Hong Kong Barcelona Budapest, pp 2236–2253Google Scholar
  5. Gillis M, Döbereiner J, Pot B, Goor M, Falsen E, Hoste B, Reinhold B, Kersters K (1991) Taxonomic relationship between (Pseudomonas) rubrisubalbicans, some clinical isolated (EF group 1) Herbaspirillum seropedicae, and (Aquaspirillum) autotrophicum. In: Pollinelli M, Materassi R, Vicenzini M (eds) Nitrogen fixation. Kluwer Academic Press, Dordrecht, pp 292–294Google Scholar
  6. Goto M (1992) Fundamentals of bacterial plant pathology. Academic Press, New YorkGoogle Scholar
  7. Hughes CG (1968) Transmission of leaf scald by soil leachate and sugarcane. Pathol Newslett 1:27–28Google Scholar
  8. Kartha KK, Gamborg OL (1975) Elimination of cassava mosaic disease by meristem culture. Phytopatology 65:826–828Google Scholar
  9. Kloepper JW, Schippers B, Bakker PAM (1992) Proposed elimination of the term endorhizosphere. Phytopathology 82:726–727Google Scholar
  10. Nesmith WC, Jenkins SF Jr (1983) Survival of Pseudomonas solanacearum in selected North Carolina Soils. Phytopathology 73:1300–1304Google Scholar
  11. Olivares FL, James EK, Reis VM, Baldani VLD, Baldani JI, Dobereiner J (1993) Colonização do tecido vascular por Herbaspirillum spp. em sorgo e cana-de-açucar. Fitopatol Bras 18:313Google Scholar
  12. Pimentel JP, Olivares FL, Pitard RM, Urquiaga S, Akiba F, Döbereiner J (1991) Dinitrogen fixation and infection of grass leaves by Pseudomonas rubrisubalbicans and Herbaspirillum seropedicae. Plant and Soil 137:1–65Google Scholar
  13. Pochon J, Tardieux P (1962) Techniques d'ánalyse en microbiologie du sol. Éditions de la Tourelle, St. Mandé (Seine). 105 pGoogle Scholar
  14. Reis VM, Olivares FL, Döbereiner J (1994) Improved methodology for isolation of Acetobacter diazotrophicus and confirmation of its endophytic habitat. World J Appl Microbiol Biotech 10:401–405Google Scholar
  15. Roszak DB, Colwell RR (1987) Survival strategies of bacteria in the natural environment. Microbiol Rev 51:365–379Google Scholar
  16. Sundaram S, Klucas RV (1988) Characterization of azospirilla isolated from seeds and roots of turf grass. Can J Microbiol 34:212–217Google Scholar
  17. Urquiaga S, Cruz KHS, Boddey R (1992) Contribution of nitrogen fixation to sugar cane: Nitrogen-15 and nitrogen balance estimates. Soil Sci Soc Am J 56:105–114Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Fabio L. Olivares
    • 1
  • Vera L. D. Baldani
    • 1
  • Veronica M. Reis
    • 1
  • Jose I. Baldani
    • 1
  • Johanna Döbereiner
    • 1
  1. 1.Embrapa-Centro Nacional de Pesquisa de AgrobiologiaRio de JaneiroBrazil

Personalised recommendations