Biology and Fertility of Soils

, Volume 11, Issue 1, pp 30–33 | Cite as

Rhizobium sp. exopolysaccharide production, nodulation, and acetylene reduction activity (N2-fixation) in pigeon pea (Cajanus cajan L.)

  • S. K. Singh
  • S. S. Dudeja
  • A. L. Khurana


There was no correlation between the quantity of exopolysaccharide produced and acetylene reduction activity by Rhizobium spp. or by Bradyrhizobium spp. (Cajanus). The exopolysaccharide-defective mutants of Rhizobium sp. strain P 116 either failed to nodulate or showed a decrease in effectiveness. The deficiency in exopolysaccharide production was corrected by the addition of purified exopolysaccharide from the parent strain, or from Bradyrhizobium sp. strain P 149 or S24 isolated from pigeonpea (Cajanus cajan) and mungbean (Vigna radiata), respectively. However, the nodules so formed were not fully effective compared to those formed by the parent strain.

Key words

Rhizobium spp. Bradyrhizobium spp. Cajanus cajan Pigeonpea Exopolysaccharide Nodulation Acetylene reduction assay 


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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • S. K. Singh
    • 1
  • S. S. Dudeja
    • 1
  • A. L. Khurana
    • 1
  1. 1.Department of MicrobiologyHaryana Agricultural UniversityHisarIndia

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