Recolonization of methyl bromide sterilized soils under four different field conditions
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Summary
The course of recovery in biological activity was assessed in the top 5 cm of undisturbed soil cores (29.7 cm diameter, 30 cm deep) that had been fumigated in the laboratory with methyl bromide. The cores were returned to their original pasture and forest sites, two with a moderate and two with a high rainfall, and untreated soils at all sites served as baselines. Sampling took place over 166 days (midsummer to midwinter). Microbial biomass (as measured by fumigation-extraction and substrate-induced respiration procedures) and dehydrogenase activity both recovered rapidly, but remained consistently lower in the fumigated than in untreated samples at both forest sites and at the moister of the two pasture sites. Bacterial numbers also recovered rapidly. Fungal hyphal lengths were, on average over 166 days, 25% lower in the fumigated soils. Levels of mineral N were initially highest in the fumigated soils, but declined with time. Fumigation generally had no detectable effects on the subsequent rates of net N mineralization and little effect on nitrification rates. Fumigation almost totally eliminated protozoa, with one to three species being recovered on day 0; the numbers recovered most rapidly in the moist forest soil and slowly in the dry pasture soil. The recoionization rate of protozoan species was similar in all soils, with species numbers on day 110 being 33 and 34 in the fumigated and untreated soils, respectively. Nematodes were eliminated by fumigation; recolonization was first detected on day 26 but by day 166, nematode numbers were still lower in fumigated than in untreated soils, the abundance being 10 and 62 g-1 soil and diversity 10 and 31 species, respectively. Overall, the results suggest that protozoan and nematode populations and diversities could provide a useful medium-term ecological index of the recovery in comprehensive soil biological activity following major soil pollution or disturbance.
Key words
Invasion Soil Recolonization Protozoa Rotifers Nematodes Microbial biomass Dehydrogenase activityPreview
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References
- Anderson JM (1988) Invertebrate-mediated transport processes in soils. Agric Ecosyst Environ 24:5–19Google Scholar
- Barth H, L'Hermite P (eds) (1987) Scientific basis for soil protection in the European community. Elsevier, London New YorkGoogle Scholar
- Blakemore LC, Searle PL, Daly BK (1987) Methods for chemical analysis of soils. NZ Soil Bur Sci Rep 80; Lower HuttGoogle Scholar
- Bonnet L (1964) Le peuplement thecamoebien des sols. Rev Écol Biol Sol 1:123–408Google Scholar
- Churchaman GJ, Tate KR (1986) Effect of slaughterhouse effluent and water irrigation upon aggregation in seasonally dry New Zealand soil under pasture. Aust J Soil Res 24:505–516Google Scholar
- Coleman DC, Anderson RV, Cole CV, McClellan JF, Woods LE, Trofymow JA, Elliott ET (1984) Roles of protozoa and nematodes in nutrient cycling. In: Giddens JE, Todd RL (eds) Microbial-plant interaction. Am Soc Agron, Madison, Wisconsin, pp 17–28Google Scholar
- Cook RJ (1986) Plant health and the sustainability of agriculture, with special reference to disease control by beneficial organisms. In: Lopez-Real JM, Hodges RD (eds) The role of microoganisms in a sustainable agriculture. AB Academic, Berkamstead, Hertfordshire, pp 125–146Google Scholar
- Elliott ET, Anderson RV, Coleman DC, Cole CV (1980) Habitable pore space and microbial trophic interactions. Oikos 35:327–335Google Scholar
- Foissner W (1987) Soil protozoa: Fundamental problems, ecological significance, adaptations in ciliates and testaceans, bioindicators, and guide to the literature. Progr Protistol 2:69–212Google Scholar
- Foissner W, Franz H, Adam H (1982) Terrestrische Protozoen als Bioindikatoren im Boden einer planierten Ski-Piste. Pedobiologia 24:45–56Google Scholar
- Gunnarsson T, Sundin P, Tunlid A (1988) Importance of leaf litter fragmentation for bacterial growth. Oikos 52:303–308Google Scholar
- Hattori T (1988) Soil aggregates as microhabitats of microoganisms. Rep Inst Agric Res Tohoku Univ 37:23–26Google Scholar
- Hooper DJ (1986) Extraction of free-living stages from soil. In: Southey JF (ed) Laboratory methods for work with plant and soil nematodes. HMSO, London, pp 5–30Google Scholar
- Ingham RE, Trofymow JA, Ingham ER, Coleman DC (1985) Interactions of bacteria, fungi and their nematode grazers: Effects on nutrient cycling and plant growth. Ecol Monogr 55:119–140Google Scholar
- Kilbertus G, Schwartz R, Alberti G (1982) La repartition quantitative des microorganismes dans le sols de fôrets (chenes, pins): Indices d'activité microbiologique. Rev Écol Biol Sol 19:513–523Google Scholar
- Lüftenegger G, Foissner W, Adam H (1985) r- and K-selection in soil ciliates: A field and experimental approach. Oecologia 66:574–579Google Scholar
- Marumoto T, Anderson JPE, Domsch KM (1982) Mineralization of nutrients from soil microbial biomass. Soil Biol Biochem 14:469–475Google Scholar
- Maw GA, Kempton RJ (1973) Methyl bromide as a soil fumigant. Soils Fert 36:41–47Google Scholar
- NZ Meteorological Service (1984) Rainfall measurements for New Zealand 1951 to 1980. NZ Met Serv Misc Publ 185, WellingtonGoogle Scholar
- Orr CC, Newton OH (1971) Distribution of nematodes by wind. Plant Dis Rep 55:61–63Google Scholar
- Paoletti MG, Iovane E, Cortese M (1988) Pedofauna bioindicators and heavy metals in five agroecosystems in north-east Italy. Rev Écol Biol Sol 25:33–58Google Scholar
- Quinn JG, Salomon M (1964) Chloride interference in the dichromate oxidation of soil hydrolysates. Proc Soil Sci Soc Am 28:456Google Scholar
- Ross DJ (1971) Some factors influencing the estimation of dehydrogenase activities of some soils under pasture. Soil Biol Biochem 3:97–110Google Scholar
- Ross DJ (1990) Estimation of soil microbial C by a fumigation-extraction method: Influence of seasons, soils, and calibration with the fumigation-incubation procedure. Soil Biol Biochem 22:295–300Google Scholar
- Ross DJ, Speir TW, Tate KR, Cairns A, Meyrick KF, Pansier EA (1982). Restoration of pasture after topsoil removal: Effects on soil carbon and nitrogen mineralization, microbial biomass and enzyme activities. Soil Biol Biochem 14:575–581Google Scholar
- Singh BN (1955) Culturing soil protozoa and estimating their numbers in soil. In: Kevan DK (ed) Soil zoology. Butterworths, London, pp 403–411Google Scholar
- Sparling GP, Feltham CW, Reynolds J, West AW, Singleton P (1990) Estimation of soil microbial C by a fumigation extraction method: Use on soils of high organic matter content and a reassessment of the kEC-factor. Soil Biochem 22:301–307Google Scholar
- Tate KR, Speir TW, Ross DJ, Parfitt RL, Whale KN, Cowling JC (1991) Temporal variations in some plant and soil P pools in two managed grassland soils of widely different P fertility status. Plant and Soil 132:219–232Google Scholar
- Thomas A, Nicholas DP, Parkinson D (1965) Modification of the agar film technique for assaying lengths of mycelium in soil. Nature (London) 205:105Google Scholar
- Walklate PJ (1989) Vertical dispersal of plant pathogens by splashing. Par I: The theoretical relationship between rainfall and upward rain splash. Plant Pathol 38:56–63Google Scholar
- West AW, Sparling GP (1986) Modifications to the substrate-induced-respiration method to permit measurement of microbial biomass in soils of differing water contents. J Microbiol Methods 5:177–189Google Scholar
- Yeates GW (1984) Variation in pasture nematode populations over thirty-six months in a summer moist silt loam. Pedobiologia 27:207–219Google Scholar