Follicle formation in the embryonic chick thyroid

II. Reorganization after dissociation
  • S. Robert Hilfer
  • Lawrence B. Iszard
  • Eva K. Hilfer


The events which occur after reaggregation of dissociated embryonic chick thyroid cells are described. Although the cells rearrange themselves into follicles within a few hours after being packed by centrifugation, their complex cytoarchitecture is not maintained. The first sign of an impending change is the accumulation of large numbers of dense bodies in the apical cytoplasm. Shortly thereafter other types of lytic bodies become numerous, and blebs containing cytoplasmic membranous components appear at the apical cell surfaces. A short time later the basal cytoplasm contains large residual bodies concomitant with a reduction in the extent of the canalicular endoplasmic reticulum and an increase in the number of free ribosomes. With the passage of time there is a progressive decrease in the size and number of residual bodies, and the channels of rough endoplasmic reticulum reappear until the normal thyroid pattern is restored. Lytic phenomena coupled with vesiculation of the endoplasmic reticulum have been linked with control of function in several organs. Therefore, the results are discussed in terms of a possible realtionship between lytic activity and the reestablishment of thyroid function after dissociation.


Endoplasmic Reticulum Thyroid Function Rough Endoplasmic Reticulum Progressive Decrease Apical Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ashford, T. P., and K. R. Porter: Cytoplasmic components in hepatic cell lysosomes. J. Cell Biol. 12, 198–202 (1962).Google Scholar
  2. Balis, J. U., and P. E. Cohen: The role of alveolar inclusion bodies in the developing lung. Lab. Invest 13, 1215–1229 (1964).Google Scholar
  3. Brandes, D., F. Bertini, and E. W. Smith: Role of lysosomes in cellular lytic processes II. Cell death during holocrine secretion in sebaceous glands. Exp. molec. Path. 4, 245–265 (1965).Google Scholar
  4. Clark, S. L.: Cellular differentiation in the kidneys of newborn mice studied with the electron microscope. J. biophys. biochem. Cytol. 3, 349–361 (1957).Google Scholar
  5. DeDuve, C., and R. Wattiaux: Functions of lysosomes. Ann. Rev. Physiol. 28, 435–492 (1966).Google Scholar
  6. Ekholm, R., and S. Smeds: On dense bodies and droplets in the follicular cells of the guinea pig thyroid. J. Ultrastruct. Res. 16, 71–82 (1966).Google Scholar
  7. Farquhar, M. G., and G. E. Palade: Cell junctions in amphibian skin. J. Cell Biol. 26, 263–291 (1965).Google Scholar
  8. Harris, C. C., and C. A. Leone: Some effects of EDTA and tetraphenylboron on the ultrastructure of mitochondria in mouse liver cells. J. Cell Biol. 28, 405–408 (1966).Google Scholar
  9. Hilfer, S. R.: Follicle formation in the embryonic chick thyroid. I. Early morphogenesis. J. Morph. 115, 135–152 (1964).Google Scholar
  10. —: Cellular interactions in the genesis and maintenance of thyroid characteristics. In: Epithelial-mesenchymal interactions (W. Fleischmajer, ed), Baltimore: Williams & Wilkins Co. p. 177–199 (1968a).Google Scholar
  11. —: Cell interactions in thyroid morphogenesis. Amer. Zool. 8, 273–284 (1968b).Google Scholar
  12. —, and E. K. Hilfer: Effects of dissociating agents on the fine structure of embryonic chick thyroid cells. J. Morph. 119, 217–231 (1966).Google Scholar
  13. — and L. B. Iszard: The relationship between cytoplasmic organization and the epitheliomesodermal interaction in the embryonic chick thyroid. J. Morph. 123, 199–212 (1967).Google Scholar
  14. -, and M. Stern: Evidence for instability of the interaction between components of the 8-day chick embryonic thyroid. To be submitted to J. exp. Zool. (1968).Google Scholar
  15. Hruban, Z., B. Spargo, H. Swift, R. W. Wissler, and R. G. Kleinfeld: Focal cytoplasmic degradation. Amer. J. Path. 42, 657–683 (1963).Google Scholar
  16. Lesseps, R. J.: Cell surface projections: Their role in the aggregation of embryonic chick cells as revealed by electron microscopy. J. exp. Zool. 153, 171–182 (1963).Google Scholar
  17. Moe, H., and O. Behnke: Cytoplasmic bodies containing mitochondria, ribosomes, and rough surfaced endoplasmic membranes in the epithelium of the small intestine of newborn rats. J. Cell Biol. 13, 168–171 (1962).Google Scholar
  18. Moscona, A. A.: Recombination of dissociated cells and the development of cell aggregates. In: Cells and Tissues in culture I. (E. N. Willmer, ed.,) p. 489–529. London: Academic Press 1966.Google Scholar
  19. Novikoff, A. B., E. Essner, and N. Quintana: Golgi apparatus and lysosomes. Fed. Proc. 23, 1010–1022 (1964).Google Scholar
  20. Palay, S. L.: The morphology of secretion. In: Frontiers in cytology (S. L. Palay, ed.), p. 305–342. New Haven: Yale University Press 1958.Google Scholar
  21. Payne, F.: A cytological study of the thyroid glands of normal and experimental fowl, including interrelationships with the pituitary, gonads and adrenals. J. Morph. 101, 89–129 (1957).Google Scholar
  22. Porter, K. R.: Changes in cell fine structure accompanying mitosis. In: Fine structure of cells, Eighth Cong. Cell Biol. Leiden 1954, p. 236–250. Groningen: P. Nordhoff 1955.Google Scholar
  23. Robbins, E., and N. K. Gonatas: The ultrastructure of a mammalian cell during the mitotic cycle. J. Cell Biol. 21, 429–463 (1964).Google Scholar
  24. Rodriguez, T. G.: Ultrastructural changes in the mouse exocrine pancreas induced by prolonged treatment with actinomycin D. J. Ultrastruct. Res. 19, 116–129 (1967).Google Scholar
  25. Saunders, J. W., and J. F. Fallon: Cell death in morphogenesis. In: Major problems in developmental Biology, 25th Symp. Soc. Devel. Biol. (M. Locke, ed.), p. 289–316. New York and London: Academic Press 1966.Google Scholar
  26. Scheib, D.: Structure fine du canal de Müller de l'embryon de Poulet: lésions cytoplasmiques du canal mâle en régression. C. R. Acad. Sci. Paris 260, 1252–1254 (1965).Google Scholar
  27. Smith, R. E., and M. G. Farquhar: Lysosome function in the regulation of the secretory process in cells of the anterior pituitary gland. J. Cell Biol. 31, 319–347 (1966).Google Scholar
  28. Sobel, H. J.: Electron microscopy of I131-irradiated thyroid. Arch. Path. 78, 53–60 (1964).Google Scholar
  29. Steinberg, M. S.: The problem of adhesive selectivity in cellular interactions. In: Cellular membranes in development, 22nd Symp. Soc. Devel. Biol. (M. Locke, ed.), p. 321–366. New York and London: Academic Press 1964.Google Scholar
  30. Swift, H., and Z. Hruban: Focal degradation as a biological process. Fed. Proc. 23, 1026–1037 (1964).Google Scholar
  31. Trinkaus, J. P.: Behavior of disassociated retinal pigment cells in heterotypic cell aggregates. Ann. N. Y. Acad. Sci. 100, 413–434 (1963).Google Scholar
  32. Weber, R.: Biochemistry of amphibian metamorphosis. In: Biochemistry of animal development, II (R. Weber, ed.) p. 227–301. New York: Academic Press 1967.Google Scholar
  33. Weiss, J. M.: The ergastoplasm. Its fine structure and relation to protein synthesis as studied with the electron microscope in the pancreas of the Swiss albino mouse. J. exp. Med. 98, 607–618 (1953).Google Scholar
  34. Wetzel, B. K., S. S. Spicer, and S. H. Wollman: Changes in fine structure and acid phosphatase localization in rat thyroid cells following thyrotopin administration. J. Cell Biol. 25, 593–618 (1965).Google Scholar
  35. Wissig, S. L.: The anatomy of secretion in the follicular cells of the thyroid gland. I. The fine structure of the gland in the normal rat. J. biophys. biochem. Cytol. 7, 419–432 (1960).Google Scholar

Copyright information

© Springer-Verlag 1968

Authors and Affiliations

  • S. Robert Hilfer
    • 1
  • Lawrence B. Iszard
    • 1
  • Eva K. Hilfer
    • 1
  1. 1.Dept. of BiologyTemple UniversityPhiladelphiaUSA

Personalised recommendations