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Archives of Toxicology

, Volume 42, Issue 4, pp 281–287 | Cite as

Determination of mixtures of urinary benzoic, 3- and 4-methylbenzoic, and mandelic acids by gas chromatography

  • H. Wieczorek
  • B. Trojanowska
  • W. Dramiński
Original Investigations

Abstract

The determination of benzoic, 3-and 4-methylbenzoic, and mandelic acids in urine by gas chromatography (G.C.) is presented. The analytical procedure includes: addition of internal standard (β-naphthol), alkaline hydrolysis, ethyl ether extraction, evaporation of the solvent, and silylation of acids with Silyl-8 in pyridine (1∶1).

The results are calculated from ratios of the internal standard and determined metabolites peak surface areas.

The coefficient of variation of the method in determination of mentioned acids is ±8%.

Key words

Metabolites of toluene, xylene, styrene Benzoic, 3-and 4-methyl-benzoic acids Mandelic acid High performance gas chromatography 

Zusammenfassung

In der Arbeit wird die Gesamtbestimmung von Benzoesäure, 3-und 4-Methylbenzoesäure sowie Mandelsäure im Harn mit der Gaschromatographie (G.C.) dargestellt. Das analytische Verfahren umfaßt die Addition des inneren Standards β-Naphthol zur Harnprobe, die alkalische Hydrolyse, die Harnextraktion mit Äthyläther, das Lösungsmittelabdämpfen sowie die Silylierung der trockenen Rückstände (von Säuren) mit dem Gemisch Silyl-8-Pyridin (1 ∶ 1).

Die Ergebnisse werden anhand der Relationen aus den Peakflächen der zu bestimmenden Metaboliten und dem inneren Standard (Standardabweichung) berechnet.

Die Genauigkeit der erarbeiteten Methode beträgt bei der Bestimmung der genannten Säuren ±8%.

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References

  1. Buchet, J. P., Lauwerys, R. R.: Measurement of urinary hippuric and m-methylhippuric acids by gas chromatography. Br. J. Ind. Med. 30, 125–128 (1973)Google Scholar
  2. Engström, K., Husman, K., Rantanen, J.: Measurement of toluene and xylene metabolites by gas chromatography. Int. Arch. Occup. Environ. Health 36, 153–160 (1976a)Google Scholar
  3. Engström, K., Härkönen, H., Kalliokoski, P., Rantanen, J.: Urinary mandelic acid concentration after occupational exposure to styrene and its use as a biological exposure test. Scand. J. Work Environ. Health 2, 21–26 (1976b)Google Scholar
  4. Flek, J., Šedivec, V.: Determination of toxic substances and their metabolites in biological fluids by gas chromatography. Coll. Chechoslov, Chem. Commun. 38, 1754–1759 (1973)Google Scholar
  5. Van der Heiden, C., Wadman, S. K., Ketting, D., de Bree P. K.: Gas Chromatographic analysis of urinary tyrosine and phenylalanine metabolites in patients with gastrointestinal disorders. Clin. Chim. Acta 34, 269–289 (1971)Google Scholar
  6. Meszka, G., Jakubowski, M.: Oznaczanie kwasu migdatowego w moczu metoda chromatografii gazowej. Medycyna Pracy 28, (4) 305–309 (1977)Google Scholar
  7. McNair, H. M., Bonelli, E. J.: Basic gas chromatography, 5th ed. Berkeley, California: Varian Aerograph 1969Google Scholar
  8. Ogata, M., Sugihara, R., Kira, S.: Quantitative determination of urinary hippuric acid and m- or p-methylhippuric acid as indices of toluene and m- or p-xylene exposure by high performance liquid chromatography. Int. Arch. Occup. Environ. Health 39, 199–206 (1977)Google Scholar
  9. Ogata, M., Tomokumi, K., Takatsuka, Y.: Quantitative determination in urine of hippuric acid and m-or p-methylhippuric acid, metabolites of toluene and m-or p-xylene. Br. J. Ind. Med. 26, 330–334 (1969)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • H. Wieczorek
    • 1
  • B. Trojanowska
    • 1
  • W. Dramiński
    • 1
  1. 1.Department of Metabolism of Toxic SubstancesInstitute of Occupational MedicineTeresyPoland

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