Current Genetics

, Volume 20, Issue 6, pp 449–452

Isolation and genetic study of p-fluoro-dl-phenylalanine-resistant mutants overproducing β-phenethyl-alcohol in Saccharomyces cerevisiae

  • Kazuro Fukuda
  • Makoto Watanabe
  • Kozo Asano
  • Kozo Ouchi
  • Seigo Takasawa
Original Articles

Summary

p-Fluoro-dl-phenylalanine (PFP)-resistant mutants which produce a large amount of β-phenethyl-alcohol, a rose-like flavor component, were isolated from the isogenic strains X2180-1A and X2180-1B of Saccharomyces cerevisiae. Cells of these mutants accumulated phenylalanine and tryptophan more than 3-fold times that of wild-type cells, while they accumulated less than half the tyrosine. The activity of prephenate dehydrogenase (PDG) (EC 1.3.1.12) was markedly decreased while that of 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase (EC 4.1.2.15) was increased. Genetic analysis revealed that the mutation occurred at the TYR1 locus, encoding PDG, and that the mutated TYR1 gene, tyr1-pfp, caused both PFP resistance and β-phenethyl-alcohol overproduction. This was supported by molecular genetic studies with cloned tyr1-pfp DNA.

Key words

Yeast Mutant p-Fluoro-dl-phenylalanine β-Phenethyl-alcohol 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Äyräpää T (1965) J Inst Brew 71:341–347Google Scholar
  2. Birnboim HC, Doly J (1979) J Bacteriol 99:222–230Google Scholar
  3. Braus G, Furter R, Prantl F, Niederberger P, Hütter R (1985) Arch Microbiol 142:383–388Google Scholar
  4. Fukuda K, Watanabe M, Asano K, Ueda H, Ohta S (1990a) Agri Biol Chem 54:269–271Google Scholar
  5. Fukuda K, Watanabe M, Asano K (1990b) Agri Biol Chem 54:3151–3156Google Scholar
  6. Ito H, Fukuda Y, Murata K, Kimura A (1983) J Bacteriol 153:163–168Google Scholar
  7. Johnston M, Davis RW (1984) Mol Cell Biol 4:1440–1448Google Scholar
  8. Mannhaupt G, Stucka R, Pilz U, Schwarzlose C, Feldmann H (1989) Gene 85:303–311Google Scholar
  9. Norrander J, Kempe T, Messing J (1983) Gene 26:101–106Google Scholar
  10. Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Science 239:487–491Google Scholar
  11. Spencer JFT, Spencer DM (1988) In: Campbell I, Duffus JH (eds) Yeast: a practical approach. IRL Press, Oxford, pp 65–106Google Scholar
  12. Teshiba S, Further R, Niederberger P, Braus G, Paravicini G, Hütter R (1986) Mol Gen Genet 205:353–357Google Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Kazuro Fukuda
    • 1
  • Makoto Watanabe
    • 1
  • Kozo Asano
    • 1
  • Kozo Ouchi
    • 2
  • Seigo Takasawa
    • 1
  1. 1.Foods and Liquors Research LaboratoriesKyowa Hakko Kogyo Co., Ltd.IbarakiJapan
  2. 2.Tokyo Research LaboratoriesKyowa Hakko Kogyo Co., Ltd.TokyoJapan

Personalised recommendations