A putative replicative form of the abutilon mosaic virus (gemini group) in a chromatin-like structure
Article
Received:
Summary
The putative replicative form of the abutilon mosaic virus (AbMV), a geminivirus, was purified from infected plants. It was shown to consist of a bipartite genome of 2660 and 2640 bp. This double-stranded DNA has a closed or relaxed circular conformation and part of it is packed in nucleoprotein complexes with a chromatin-like structure. Similarities between the geminiviruses and the animal simian virus 40 are discussed against this back-ground.
Key words
Abutilon mosaic virus Geminivirus cccDNA ChromatinPreview
Unable to display preview. Download preview PDF.
References
- Abouzid AM, Jeske H (1986) The purification and characterization of gemini particles from abutilon mosaic virus infected Malvaceae. J Phytopathol 115:344–353Google Scholar
- Brack C (1981) DNA electron microscopy. Crit Rev Biochem 10:113–169Google Scholar
- Griffith JD (1975) Chromatin structure: Deduced from a minichromosome. Science 187:1202–1203Google Scholar
- Gröning BR, Abouzid A, Jeske H (1987) Single-stranded DNA from abutilon mosaic virus (AbMV) is present in the plastids of infected Abutilon sellovianum. Proc Natl Acad Sci USA, 84:8996–9000Google Scholar
- Hamilton WDO, Bisaro BM, Coutts RHA, Buck KW (1983) Demonstration of the bipartite nature of the genome of a single-stranded DNA plant virus by infection with the cloned DNA components. Nucleic Acids Res 11:7387–7391Google Scholar
- Harrison BD (1985) Advances in geminivirus research. Annu Rev Phytopathol 23:55–82Google Scholar
- Howarth AJ, Caton J, Bossert M, Goodman RM (1985) Nucleotide sequence of bean golden mosaic virus and a model for gene regulation in geminiviruses. Proc Natl Acad Sci USA 82:3572–3576Google Scholar
- Ikegami M, Haber S, Goodman RM (1981) Isolation and characterization of virus-specific double-stranded DNA from tissues infected by bean golden mosaic virus. Proc Natl Acad Sci USA 78:4102–4106Google Scholar
- Jeske H (1986) The detection of the abutilon mosaic virus depends on the experimental daytime. J Phytopathol 115:243–256Google Scholar
- Jeske H, Schuchalter-Eicke G (1984) The abutilon mosaic virus (AbMV) in different leaf tissue of several host plant species of Malvaceae. Phytopath Z 109:353–362Google Scholar
- Jeske H, Werz G (1978) The influence of light intensity on pigment composition and ultrastructure of plastids in leaves of diseased Abutilon sellowianum Reg. Phytopath Z 91:1–13Google Scholar
- Jeske H, Werz G (1980a) Ultrastructural and biochemical investigations on the whitefly transmitted abutilon mosaic virus (AbMV). Phytopath Z 97:43–55Google Scholar
- Jeske H, Werz G (1980b) Cytochemical characterization of plastidal inclusions in abutilon mosaic infected Malva parviflora mesophyll cells. Virology 106:155–158Google Scholar
- Jeske H, Menzel D, Werz G (1977) Electron microscopic studies on intranuclear virus-like inclusions in mosaic-diseased Abutilon sellowianum. Reg Phytopath Z 89:289–295Google Scholar
- Kleinschmidt A, Lang D, Plescher C, Hellmann W, Haass J, Zahn RK, Hagedorn A (1961) Über die intrazelluläre Formation von Bakterien-DNS. Z Naturforschung 16b:730–739Google Scholar
- Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning — a laboratory manual. Cold Spring Harbor Laboratory NYGoogle Scholar
- Müller U, Zentgraf H, Eicken I, Keller W (1978) Higher-order structure of simian virus 40 chromatin. Science 201:406–415Google Scholar
- Schaffer HE, Sederoff R (1981) Improved estimation of DNA fragment lengths from agarose gels. Anal Biochem 115:113–122Google Scholar
- Schuchalter-Eicke G, Jeske H (1983) Seasonal changes in the chloroplast ultrastructure in Abutilon Mosaic Virus (AbMV) infected Abutilon spec. (Malvaceae). Phytopath Z 108:172–184Google Scholar
- Southern E (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503Google Scholar
- Stanley J (1983) Infectivity of the cloned geminivirus genome requires sequences from both DNAs. Nature 305:643–645Google Scholar
- Stanley J, Davies JW (1985) Structure and function of the DNA genome of geminiviruses. In: Davies J (ed) Molecular plant virology. CRC Press, Boca Raton, pp 191–218Google Scholar
- Stanley J et al. (1986) The nucleotide sequence of an infections clone of the geminivirus beet curly top virus. Embo J 5:1761–1767Google Scholar
- Stanley J, Townsend R (1985) Characterisation of DNA forms associated with cassava latent virus infection. Nucleic Acids Res 13:2189–2207Google Scholar
- Sunter G, Coutts RHA, Buck KW (1984) Negatively supercoiled DNA from plants infected with a single-stranded DNA virus. Biochem Biophys Res Commun 118:747–752Google Scholar
Copyright information
© Springer-Verlag 1988