Advertisement

Molecular and General Genetics MGG

, Volume 216, Issue 2–3, pp 503–510 | Cite as

Identification of the recR locus of Escherichia coli K-12 and analysis of its role in recombination and DNA repair

  • Akeel A. Mahdi
  • Robert G. Lloyd
Article

Summary

A new recombination gene called recR has been identified and located near dnaZ at minute 11 on the current linkage map of Escherichia coli. The gene was detected after transposon mutagenesis of a recB sbcB sbcC strain and screening for insertion mutants that had a reduced efficiency of recombination in Hfr crosses. The recR insertions obtained conferred a recombination deficient and extremely UV sensitive phenotype in both recB recC sbcA and recB recC sbcB sbcC genetic backgrounds. recR derivatives of recBC+sbc+ strains were proficient in conjugational and transductional recombination but deficient in plasmid recombination and sensitive to UV light. Strains carrying recR insertions combined with mutations uvrA and other rec genes revealed that the gene is involved in a recombinational process of DNA repair that relies also on recF and recO, and possibly recJ, but which is independent of recB, recC and recD. The properties of two other insertions, one located near pyrE and the other near guaA, are discussed in relation to their proximity to recG and xse (the gene for exonuclease VII), respectively.

Key words

Escherichia coli recR Recombination DNA repair 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bachmann BJ (1987a) Linkage map of Escherichia coli K-12, edition 7. In: Neidhardt FC, Ingraham JL, Low KB, Magasanik B, Schaechter M, Umbarger HE (eds) Escherichia coli and Salmonella typhimurium cellular and molecular biology. American Society for Microbiology, Washington DC, pp 807–876Google Scholar
  2. Bachmann BJ (1987b) Derivations and genotypes of some mutant derivatives of Escherichia coli K-12. In: Neidhart FC, Ingraham JL, Low KB, Magasanik B, Schaechter M, Umbarger HE (eds) Escherichia coli and Salmonella typhimurium cellular and molecular biology. American Society for Microbiology, Washington DC, pp 1190–1219Google Scholar
  3. Chase JW, Richardson CC (1977) Escherichia coli mutants deficient in exonuclease VII. J Bacteriol 129:934–947Google Scholar
  4. Clark AJ, Low KB (1988) Pathways and systems of homologous recombination in Escherichia coli. In: Low KB (ed) The Recombination of Genetic Material. Academic Press, New York London, pp 155–215Google Scholar
  5. Horii ZI, Clark AJ (1973) Genetic analysis of the RecF pathway to genetic recombination in Escherichia coli K12: isolation and characterisation of mutants. J Mol Biol 80:327–344Google Scholar
  6. Howard-Flanders P, Theriot L, Stedeford JB (1969) Some properties of excision-defective recombination-deficient mutants of Escherichia coli K-12. J Bacteriol 97:1134–1141Google Scholar
  7. Kolodner R, Fishel RA, Howard M (1985) Genetic recombination of bacterial plasmid DNA: Effect of RecF pathway mutations on plasmid recombination in Escherichia coli. J Bacteriol 163:1060–1066Google Scholar
  8. Lloyd RG, Buckman C (1985) Identification and genetic analysis of sbcC mutations in commonly used recBC sbcB strains of Escherichia coli K-12. J Bacteriol 164:836–844Google Scholar
  9. Lloyd RG, Benson FE, Shurvinton CE (1984) Effect of ruv mutations on recombination and DNA repair in Escherichia coli. Mol Gen Genet 194:303–309Google Scholar
  10. Lloyd RG, Buckman C, Benson FE (1987a) Genetic analysis of conjugational recombination in Escherichia coli K12 strains deficient in RecBCD enzyme. J Gen Microbiol 133:2531–2538Google Scholar
  11. Lloyd RG, Evans NP, Buckman C (1987b) Formation of recombinant lacZ + DNA in conjugational crosses with a recB mutant of Escherichia coli K12 depends on recF, recJ, and recO. Mol Gen Genet 209:135–141Google Scholar
  12. Lloyd RG, Porton MC, Buckman C (1988) Effect of recF, recJ, recN, recO and ruv mutations on ultraviolet survival and genetic recombination in a recD strain of Escherichia coli K12. Mol Gen Genet 212:317–324Google Scholar
  13. Lovett ST, Luisi-DeLuca C, Kolodner RD (1988) The genetic dependence of recombination in recD mutants of Escherichia coli. Genetics 120:37–45Google Scholar
  14. Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New YorkGoogle Scholar
  15. Rosenberg SM (1987) Chi-stimulated patches are heteroduplex, with recombinant information on the λ r chain. Cell 48:855–865Google Scholar
  16. Rosenberg SM (1988) Chain-bias of E. coli Rec-mediated λ patch recombinants is independent of the orientation of λ cos. Genetics 120:7–21Google Scholar
  17. Silhavy TJ, Berman ML, Enquist LW (1984) Experiments with gene fusions. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New YorkGoogle Scholar
  18. Smith GR (1987) Mechanism and control of homologous recombination in Escherichia coli. Annu Rev Genet 21:179–201Google Scholar
  19. Smith GR (1988) Homologous recombination in prokaryotes. Microbiol Rev 52:1–28Google Scholar
  20. Storm PK, Hoekstra WPM, De Haan PG, Verhoeff C (1971) Genetic recombination in Escherichia coli IV. Isolation and characterization of recombination-deficient mutants of Escherichia coli K12. Mutat Res 13:9–17Google Scholar
  21. Walker GC (1984) Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli. Microbiol Rev 48:60–93Google Scholar
  22. Wang TV, Smith KC (1988) Different effects of recJ and recN mutations on the postreplication repair of UV-damaged DNA in Escherichia coli K-12. J Bacteriol 170:2555–2559Google Scholar
  23. Way JC, Davis MA, Morisato D, Roberts DE, Kleckner N (1984) New Tn10 derivatives for construction of lacZ operon fusions by transposition. Gene 32:369–379Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Akeel A. Mahdi
    • 1
  • Robert G. Lloyd
    • 1
  1. 1.Department of GeneticsUniversity of Nottingham, Queens Medical CentreNottinghamUK

Personalised recommendations