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Molecular and General Genetics MGG

, Volume 216, Issue 2–3, pp 408–411 | Cite as

Transformation of a nitrate reductase deficient mutant of Penicillium chrysogenum with the corresponding Aspergillus niger and A. nidulans niaD genes

  • Michael P. Whitehead
  • Shiela E. Unkles
  • Martin Ramsden
  • Edward I. Campbell
  • Sarah Jane Gurr
  • David Spence
  • Cees van den Hondel
  • Roland Contreras
  • James R. Kinghorn
Article

Summary

A heterologous gene mediated transformation system based on niaD, the structural gene encoding nitrate reductase, has been developed for Penicillium chrysogenum. Transformation frequencies of up to 20 transformants per microgram DNA were obtained using the Aspergillus nidulans gene and 9 transformants per microgram using the A. niger gene. Vector constructs carrying the A. nidulans ans-1 sequence and the A. niger niaD gene did not show increased transformation frequencies. Southern blot hybridisation analysis demonstrated that vector sequences had integrated into the recipient genome. The control of heterologous niaD gene expression generally agreed with that found in the wild-type strain, that is, induction by nitrate and repression in the presence of ammonium.

Key words

Penicillium chrysogenum Nitrate reductase niaD mutants Nitrate assimilation Fungal transformation 

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Michael P. Whitehead
    • 1
  • Shiela E. Unkles
    • 1
  • Martin Ramsden
    • 2
  • Edward I. Campbell
    • 1
  • Sarah Jane Gurr
    • 1
  • David Spence
    • 2
  • Cees van den Hondel
    • 3
  • Roland Contreras
    • 4
  • James R. Kinghorn
    • 1
  1. 1.Plant Molecular Genetics Unit, Sir Harold Mitchell BuildingSt. AndrewsUK
  2. 2.Genetic LaboratoryGlaxochem Ltd.UlverstonUK
  3. 3.Medical Biological Laboratory TNORijswijkThe Netherlands
  4. 4.Laboratorium voor Moleculaire BiologieUniversity of GentGentBelgium

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