Molecular and General Genetics MGG

, Volume 185, Issue 3, pp 506–509

Cell cycle inhibition of yeast spheroplasts

  • Seishi Murakami
  • Dennis M. Livingston
Short Communication


Osmotically stabilized yeast spheroplasts are capable of extensive DNA synthesis. Although the rate of DNA synthesis in spheroplasts is approximately one-third that of intact cells, the relative amounts of nuclear and mitochondrial DNA synthesized by spheroplasts is very similar to the relative amounts synthesized by intact cells. Furthermore, nuclear but not mitochondrial DNA synthesis is inhibited in MATa spheroplasts by the application of the yeast mating pheromone, α-factor. Similarly, DNA synthesis is reversibly temperature-sensitive in spheroplasts created from cdc7 and cdc8 mutant cells.


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  1. Beggs JD (1978) Transformation of yeast by a replicating hybrid plasmid. Nature 275:104–109Google Scholar
  2. Bucking-Throm E, Duntze W, Hartwell LH, Manney TR (1973) Reversible arrest of haploid yeast cells at the initiation of DNA synthesis by a diffusible sex factor. Exp Cell Res 76:99–110Google Scholar
  3. Cottrell S, Rabinowitz M, Getz GS (1973) Mitochondrial deoxyribonucleic acid synthesis in a temperature-sensitive mutant of deoxyribonucleic acid replication in Saccharomyces cerevisiae. Biochemistry 12:4374–4378Google Scholar
  4. Cryer DR, Goldthwaite CD, Zinker S, Lam K-B, Storm E, Hirschberg R, Blamire J, Finkelstein DB, Marmur J (1973) Studies on nuclear and mitochondrial DNA of Saccharomyces cerevisiae. Cold Spring Harbor Symp Quant Biol 38:17–29Google Scholar
  5. Doi A, Doi K (1979) Mitotic behavior of yeast spheroplasts in liquid culture. Cell Structure and Function 4:261–266Google Scholar
  6. Hartwell LH (1967) Macromolecular synthesis in temperature-sensitive mutants of yeast. J Bacteriol 93:1662–1670Google Scholar
  7. Hartwell LH (1970) Periodic density fluctuation during the yeast cell cycle and the selection of synchronous cultures. J Bacteriol 104:1280–1285Google Scholar
  8. Hartwell LH (1971) Genetic control of the cell division cycle in yeast: Genes controlling DNA replication and its initiation. J Mol Biol 59:183–194Google Scholar
  9. Hartwell LH (1973) Three additional genes required for deoxyribonucleic acid synthesis in Saccharomyces cerevisiae. J Bacteriol 115:966–974Google Scholar
  10. Hartwell LH, Mortimer RK, Culotti J, Culotti M (1973) Genetic control of the cell division cycle in yeast: genetic analysis of cdc mutants. Genetics 74:267–286Google Scholar
  11. Hinnen A, Hicks JB, Fink GR (1978) Transformation of yeast. Proc Natl Acad Sci USA 75:1929–1933Google Scholar
  12. Hutchinson HT, Hartwell LH (1967) Macromolecule synthesis in yeast spheroplasts. J Bacteriol 74:1697–1705Google Scholar
  13. Murakami S, Bodley JW, Livingston DM (1982) Yeast spheroplasts are sensitive to the action of diphtheria toxin. Mol Cell Biol (in press)Google Scholar
  14. Newlon CS, Fangman WL (1975) Mitochondrial DNA synthesis in cell cycle mutants of Saccharomyces cerevisiae. Cell 5:423–428Google Scholar
  15. Oertel W, Goulian M (1977) Deoxyribonucleic acid synthesis in permeabilized spheroplasts of Saccharomyces cerevisiae. J Bacteriol 132:233–246Google Scholar
  16. Petes TD, Fangman WL (1973) Prefarential synthesis of yeast mitochrondrial DNA in α-factor arrested cells. Biochem Biophys Res Commun 55:603–609Google Scholar
  17. Throm E, Duntze W (1970) Mating-type-dependent inhibition of deoxyribonucleic acid synthesis in Saccharomyces cerevisiae. J Bacteriol 104:1388–1390Google Scholar
  18. Yee WS, Decker RW, Brunk CF (1976) Incorporation of tritiumlabeled thymidine monophosphate into nuclear DNA by permeabilized yeast cells. Biochim Biophys Acta 447:385–390Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Seishi Murakami
    • 1
  • Dennis M. Livingston
    • 1
  1. 1.Department of BiochemistryUniversity of MinnesotaMinneapolisUSA

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