Molecular and General Genetics MGG

, Volume 185, Issue 3, pp 445–447

An amber mutation in the gene rpsA for ribosomal protein S1 in Escherichia coli

  • Madoka Kitakawa
  • Katsumi Isono
Article

DOI: 10.1007/BF00334137

Cite this article as:
Kitakawa, M. & Isono, K. Mol Gen Genet (1982) 185: 445. doi:10.1007/BF00334137

Summary

An amber mutation has been induced in the gene rpsA (which codes fo ribosomal protein S1) of Escherichia coli K-12 strain in the presence of an amber suppressor (supD) and mutations sueA, sueB and sueC that additively enhance the efficiency of suppression. That the amber mutation has occurred in the gene rpsA was confirmed by complementation with a plasmid which carried the wild-type allele of rpsA. The mutation is lethal in the absence of an amber suppressor, indicating that ribosomal protein S1 is indispensable to E. coli.

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Madoka Kitakawa
    • 1
  • Katsumi Isono
    • 1
  1. 1.Abt. WittmannMax-Planck-Institut für Molekulare GenetikBerlin 33

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