Molecular and General Genetics MGG

, Volume 185, Issue 3, pp 445–447

An amber mutation in the gene rpsA for ribosomal protein S1 in Escherichia coli

  • Madoka Kitakawa
  • Katsumi Isono


An amber mutation has been induced in the gene rpsA (which codes fo ribosomal protein S1) of Escherichia coli K-12 strain in the presence of an amber suppressor (supD) and mutations sueA, sueB and sueC that additively enhance the efficiency of suppression. That the amber mutation has occurred in the gene rpsA was confirmed by complementation with a plasmid which carried the wild-type allele of rpsA. The mutation is lethal in the absence of an amber suppressor, indicating that ribosomal protein S1 is indispensable to E. coli.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Christiansen L, Pedersen S (1981) Cloning, restriction endonuclease mapping and post-transcriptional regulation of rpsA, the structural sene for ribosomal protein S1. Mol Gen Genet 181:548–551Google Scholar
  2. Isono K, Isono S (1976) Lack of ribosomal protein S1 in Bacillus stearothermophilus. Proc Natl Acad Sci USA 73:767–770Google Scholar
  3. Kimura M, Foulaki K, Subramanian A, Wittmann-Liebold B (1982) Primary structure of Escherichia coli ribosomal protein S1 and features of its functional domains. Eur J Biochem 123:37–53Google Scholar
  4. Kimura M, Miki T, Hiraga S, Nagata T, Yura T (1979) Conditionally lethal amber mutations in the dnaA region of the Escherichia coli chromosome that affect chromosome replication. J Bacteriol 140:825–834Google Scholar
  5. Kitakawa M, Blumenthal L, Isono K (1980) Isolation and characterization of specialized transducing λ phages carrying ribosomal protein genes of Escherichia coli. Mol Gen Genet 180:343–349Google Scholar
  6. Murray NE, Brammar WJ, Murray K (1977) Lambdoid phage that simplify the recovery of in vitro recombinants. Mol Gen Genet 150:53–61Google Scholar
  7. Oeschger MP, Oeschger NS, Wiprud GT, Woods SL (1980) High efficiency temperature-sensitive amber suppressor strains of Escherichia coli: isolation of strains with suppressor-enhancing mutations. Mol Gen Genet 177:545–552Google Scholar
  8. Oeschger MP, Wiprud GT (1980) High efficiency temperature-sensitive amber suppressor strains of Escherichia coli K-12. Mol Gen Genet 178:293–299Google Scholar
  9. Russell RL, Abelson JN, Landy A, Gefter ML, Brenner S, Smith JD (1970) Duplicate genes for tyrosine transfer RNA in Escherichia coli. J Mol Biol 47:1–13Google Scholar
  10. Schnier J, Isono K (1982) The DNA sequence of the gene rpsA of Escherichia coli coding for ribosomal protein S1. Nucl Acids Res (in press)Google Scholar
  11. Schnier J, Kimura M, Foulaki K, Subramanian AR, Isono K, Wittmann-Liebold B (1981) The primary structure of Escherichia coli ribosomal protein S1 and of its gene rpsA. Proc Natl Acad Sci USA 79:1008–1011Google Scholar
  12. Schrenk WJ, Weisberg RA (1975) A simple method for making new transducing lines of coliphage λ. Mol Gen Genet 137:101–107Google Scholar
  13. Steitz JA, Jakes K (1975) How ribosomes select intiator regions in mRNA: Base pair formation between the 3′ terminus of 169 rRNA and the mRNA during initiation of protein synthesis in Escherichia coli. Proc Natl Acad Sci USA 72:4724–4728Google Scholar
  14. Subramanian AR, Mizushima S (1979) Characterization of a mutant form of ribosomal protein S1 from Escherichia coli. J Biol Chem 254:4309–4312Google Scholar
  15. Szer W, Hermoso JM, Boublik M (1976) Destabilization of the secondary structure of RNA by ribosomal protein S1 from Escherichia coli. Biochem Biophys Res Commun 70:957–964Google Scholar
  16. Wahba AJ, Miller MJ, Nieveleau A, Landers TA, Carmichael GG, Weber K, Hawley DA, Slobin LI (1974) Subunit I of Qβ replicase and 30S ribosomal protein S1 of Escherichia coli: Evidence for the identity of the two proteins. J Biol Chem 249:3314–3316Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Madoka Kitakawa
    • 1
  • Katsumi Isono
    • 1
  1. 1.Abt. WittmannMax-Planck-Institut für Molekulare GenetikBerlin 33

Personalised recommendations