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Molecular and General Genetics MGG

, Volume 185, Issue 1, pp 136–141 | Cite as

Regulation of L-arabinose transport in Salmonella typhimurium LT2

  • Jar-How Lee
  • Robert J. Russo
  • Laurel Heffernan
  • Gary Wilcox
Article

Summary

The inducible L-arabinose transport system was characterized in Salmonella typhimurium LT2. Only one L-arabinose transport system with a Km of 2x10-4 M was identified. The results suggested that araE may be the only gene which codes for L-arabinose transport activity under the conditions tested. An araE-lac fusion strain was used to study the induction of the araE gene. No araE expression was detected when the L-arabinose concentration was lower than 1 mM. The expression of araE reached a maximum in the presence of 50 mM L-arabinose, and was significantly reduced in the presence of D-glucose. Expression of the araBAD and araE genes was coordinately regulated. The concentration of L-arabinose that allowed maximum araBAD gene expression was 50-fold lower in an araE+ strain compared to an araE strain.

Keywords

Gene Expression Transport System Transport Activity Fusion Strain araE Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Jar-How Lee
    • 1
    • 2
  • Robert J. Russo
    • 1
    • 2
  • Laurel Heffernan
    • 1
    • 2
  • Gary Wilcox
    • 1
    • 2
  1. 1.Department of Microbiology, College of Letters and ScienceUniversity of CaliforniaLos AngelesUSA
  2. 2.Molecular Biology InstituteUniversity of CaliforniaLos AngelesUSA

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